Mitophagy reporter mouse analysis reveals increased mitophagy activity in disuse‐induced muscle atrophy

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Abstract
Muscle disuse induces atrophy through increased reactive oxygen species (ROS) released from damaged mitochondria. Mitophagy, the autophagic degradation of mitochondria, is associated with increased ROS production. However, the mitophagy activity status during disuse‐induced muscle atrophy has been a subject of debate. Here, we developed a new mitophagy reporter mouse line to examine how disuse affected mitophagy activity in skeletal muscles. Mice expressing tandem mCherry‐EGFP proteins on mitochondria were then used to monitor the dynamics of mitophagy activity. The reporter mice demonstrated enhanced mitophagy activity and increased ROS production in atrophic soleus muscles following a 14‐day hindlimb immobilization. Results also showed an increased expression of multiple mitophagy genes, including Bnip3, Bnip3l, and Park2. Our findings thus conclude that disuse enhances mitophagy activity and ROS production in atrophic skeletal muscles and suggests that mitophagy is a potential therapeutic target for disuse‐induced muscle atrophy.
Funding Information
  • Japan Agency for Medical Research and Development (JP18gm6110013h0001)
  • Takeda Science Foundation
  • Japan Society for the Promotion of Science (16H01198, 16KK0162, 17H03671, 17K15088, 18H04691, 18H04858, 19H05712, 19K23822, 20H04035, 20K21521)
  • Suzuken Memorial Foundation

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