EFFECT OF PORCINE BRAIN NATRIURETIC PEPTIDE (pBNP) ON HUMAN ADRENOCORTICAL STEROIDOGENESIS

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Abstract
Porcine brain natriuretic peptide (pBNP), purified from porcine brain, had a significant suppressive effect on aldosterone and cortisol secretions in ACTH‐treated cultured human adrenal cells. Concomitantly, the intracellular cGMP formation was enhanced by pBNP treatment. A specific pBNP receptor was identified in the human adrenal tissues. Affinity labelling of 125I‐BNP showed two separate molecular weights of specific binding sites for pBNP of 140 and 67 kDa. A 125I‐BNP binding study of the human adrenal membrane fraction demonstrated the presence of high‐affinity and low‐capacity binding sites for pBNP. Moreover, these binding sites for 125I‐pBNP were displaced by unlabelled α‐rANP as well as pBNP. From these studies, we concluded that pBNP had suppressive effects on human adrenocortical steroidogensis, possibly via a receptor which may be shared with ANP.

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