Greater Susceptibility to Mutations in Lagging Strand of DNA Replication in Escherichia coli than in Leading Strand
Authors:
- 30 July 1993
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 261 (5121) , 598-600
Abstract
Models of DNA replication in Escherichia coli involve an asymmetric DNA polymerase complex that replicates concurrently the leading and the lagging strands of double-stranded DNA. The effect of asymmetry on mutagenesis was tested with pairs of plasmids containing the unidirectional ColE1 origin of replication and a single lesion located in the leading or lagging strand. The lesion used was the covalent adduct that the chemical carcinogen N -2-acetylaminofluorene (AAF) forms with the C-8 position of guanine. Whether SOS was induced or not, mutations arose at about a 20-fold higher frequency when the AAF adduct was located in the lagging strand than when in the leading strand.Keywords
This publication has 14 references indexed in Scilit:
- DNA adduct-induced stabilization of slipped frameshift intermediates within repetitive sequences: implications for mutagenesis.Proceedings of the National Academy of Sciences, 1993
- PROKARYOTIC DNA REPLICATIONAnnual Review of Biochemistry, 1992
- Preferential DNA secondary structure mutagenesis in the lagging strand of replication in E. coliNature, 1991
- DNA Mismatch Correction in a Defined SystemScience, 1989
- Single adduct mutagenesis: strong effect of the position of a single acetylaminofluorene adduct within a mutation hot spot.Proceedings of the National Academy of Sciences, 1989
- Genetic control of AAF-induced mutagenesis at alternating GC sequences: An additional role for RecAMolecular Genetics and Genomics, 1989
- Carcinogen-induced mutation spectrum in wild-type, uvrA and umuC strains of Escherichia coliJournal of Molecular Biology, 1984
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Hot spots of frameshift mutations induced by the ultimate carcinogen N- acetoxy-N-2-acetylaminofluoreneNature, 1981
- Replication of Colicin E1 Plasmid DNA in Cell Extracts. Origin and Direction of ReplicationProceedings of the National Academy of Sciences, 1974