Three-dimensional structure of ubiquitin at 2.8 A resolution.
Authors:
- S Vijay-KumarSVS Vijay-Kumar
- C E BuggCBC E Bugg
- K D WilkinsonKWK D Wilkinson
- W J CookWCW J Cook
- 1 June 1985
- journal article
- research article
- Published by National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (11) , 3582-3585
Abstract
The three-dimensional structure of ubiquitin has been determined at 2.8 A resolution. X-ray diffraction data for the native protein and derivatives were collected with an automated diffractometer. Phases were obtained by use of a single isomorphous mercuric acetate derivative. The molecule contains a pronounced hydrophobic core. Prominent secondary structural features include three and one-half turns of alpha-helix, a mixed beta-sheet that contains four strands, and seven reverse turns. The histidine, tyrosine, and two phenylalanine residues are located on the surface of the molecule.Keywords
This publication has 27 references indexed in Scilit:
- The ubiquitin‐mediated proteolytic pathway and mechanisms of energy‐dependent intracellular protein degradationJournal of Cellular Biochemistry, 1984
- The chemistry and biology of thymosin. I. Isolation, characterization, and biological activities of thymosin alpha1 and polypeptide beta1 from calf thymus.Journal of Biological Chemistry, 1979
- The chemistry and biology of thymosin. II. Amino acid sequence analysis of thymosin alpha1 and polypeptide beta1.Journal of Biological Chemistry, 1979
- Early Stages of Human Marrow Lymphocyte Differentiation: Induction in Vitro by Thymopoietin and UbiquitinThe Journal of Immunology, 1979
- Free ubiquitin is a non-histone protein of trout testis chromatinNature, 1978
- Nuclear magnetic resonance studies of the denaturation of ubiquitinBiochimica et Biophysica Acta (BBA) - Protein Structure, 1977
- INDUCTION OF HUMAN GRANULOCYTE DIFFERENTIATION INVITRO BY UBIQUITIN AND THYMOPOIETIN1977
- Amino-terminal sequence identity of ubiquitin and the nonhistone component of nuclear protein A24Biochemical and Biophysical Research Communications, 1977
- Complete amino acid sequence of ubiquitin, an adenylate cyclase stimulating polypeptide probably universal in living cellsBiochemistry, 1975
- Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells.Proceedings of the National Academy of Sciences, 1975