Limitations and possibilities of low cell number ChIP-seq
Open Access
- 1 January 2012
- journal article
- Published by Springer Nature in BMC Genomics
- Vol. 13 (1) , 645
Abstract
Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq) offers high resolution, genome-wide analysis of DNA-protein interactions. However, current standard methods require abundant starting material in the range of 1–20 million cells per immunoprecipitation, and remain a bottleneck to the acquisition of biologically relevant epigenetic data. Using a ChIP-seq protocol optimised for low cell numbers (down to 100,000 cells / IP), we examined the performance of the ChIP-seq technique on a series of decreasing cell numbers.Keywords
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