Ca2+‐dependent translocation of cytosolic proteins to isolated granule subpopulations and plasma membrane from human neutrophils
Authors:
- 15 June 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 304 (2-3) , 195-197
Abstract
In order to identify cytosolic proteins involved in control of granule exocytosis in human neutrophils, subcellular fractions enriched in each of the 3 major granule subsets were incubated with cytosol from neutrophils in the presence or absence of Ca2+. After washing, proteins were eluted from the organelles by EGTA, Annexins I, II, IV and VI were found to bind to all organelles studied. In addition, a 28‐kDa protein was found to bind exclusively to plasma membranes and secretory vesicles, the most readily exocytosed organelle of neutrophils. Ca2+‐dependent association of cytosolic proteins to different granule subsets may control differential exocytosis of granules.Keywords
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