In VitroFormation of Crocetin Glucosyl Esters byCrocus sativusCallus Extract

Authors:
  • FCFrançois Cormier
    François Cormier
  • SDSonia Dorion
    Sonia Dorion
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Abstract
Callus extracts of Crocus sativus exhibited the ability to transform all-trans-crocetin into its related glycosides between pH 7.0 and 7.6 in the presence of uridine-diphospho-glucose. The reactions involved the step-wise addition of a glucose moiety to a free car boxyl function and the 1→6 addition of a glucose moiety to a glucosyl ester function. The kinetics of synthesis for each glycoside seemed to indicate that two distinct glucosyl transferases were implicated in the synthesis of crocin, all-trans-crocetin di-(β-D-gentiobiosyl) ester.