Tatjana Dostalova

This article introduces a modified reverse scan technique for abutment teeth that incorporates laboratory scanners into a fully digital workflow to enhance accuracy and reduces the impact of stitching and merging errors associated with long-span intraoral scans when fabricating extensive tooth-supported prostheses. This cost-effective, time-saving, and versatile procedure uses a 3D-printed interim tooth-supported prosthesis that is sectioned, bonded intraorally, and scanned with a laboratory scanner to improve the virtual definitive cast. The modification is performed using free software.

The increasing demand for better dental aesthetics has driven the development of tooth-whitening techniques that are effective while reducing invasiveness. Hydrogen peroxide (HP) and carbamide peroxide (CP) continue to be the most common active ingredients in bleaching products. Various types of light and laser activation have been introduced to speed up the bleaching process and decrease clinical application time. However, published results regarding their effectiveness and biological safety are inconsistent and sometimes contradictory. Aim: The objective of this study was to identify irradiation conditions that optimise the whitening performance of peroxide-based bleaching agents while ensuring safety for dental hard tissues and ocular structures. This objective was achieved through a systematic synthesis and meta-analyses of both experimental and clinical evidence on bleaching techniques, light or laser activation, and related treatment outcomes. Additionally, the study aimed to provide an integrated overview of currently used irradiation technologies, bleaching agents, treatment protocols, and relevant safety considerations. Methods: A multi-stage analytical approach was employed. Evidence was collected from systematic reviews, randomised and non-randomised clinical trials, and laboratory-based in vitro investigations. The studies assessed differences in bleaching agents (HP and CP), their concentrations, and application protocols, as well as various activation systems, including halogen lamps, conventional LEDs, violet LEDs, metal–halide lamps, and laser wavelengths such as visible blue (~440 nm), red or near-infrared (~1.7 µm), and other spectral ranges. Extracted outcome measures included tooth colour improvement (ΔSGU, ΔE), incidence of tooth sensitivity, changes in enamel surface morphology, temperature increases in the pulp chamber, and the bond strength of restorative or orthodontic materials. When methodological compatibility permitted, quantitative synthesis and meta-analysis were conducted to estimate the effects of activation modalities and irradiation parameters. Results: Analysis of data from 28 systematic reviews and numerous clinical and laboratory studies showed that the degree of colour improvement did not consistently rely on peroxide concentration or on whether bleaching was performed in-office or through home-based protocols. In most studies, adding light activation did not produce a clearly superior whitening effect compared to chemically driven bleaching alone. However, certain laser-assisted methods—especially those using blue diode lasers around 440 nm or near-infrared diode lasers near 1.7 µm—were linked with faster whitening responses and, in several in vitro experiments, fewer enamel surface irregularities. Increases in pulp temperature remained below the generally accepted safety threshold of 5.5 °C in the reported experimental conditions. While laser activation reduced treatment time, some studies observed a temporary decrease in the bond strength of orthodontic brackets following bleaching. Photobiomodulation techniques seem promising for reducing post-treatment sensitivity, although more robust clinical evidence is still needed. Conclusions: Targeted activation with diode lasers, especially within the blue and near-infrared spectral ranges, may speed up the whitening process and potentially minimise structural changes to enamel when irradiation parameters are carefully managed. Despite these positive findings, current clinical evidence remains limited. Well-designed randomised controlled trials with standardised treatment protocols are essential to determine the best wavelengths, energy delivery settings, and safety limits for laser-assisted dental bleaching.

Patients with rare disease-related orofacial dysmorphic features receive more suitable long-term functional and aesthetic dental reconstruction, including implant insertions. Treatments utilizing 3D planning and implementation of AI in the process enable precise and generally noninvasive multidisciplinary therapy. This therapeutic strategy is more cost-effective and markedly improves patient adherence and, thus, the overall therapeutic outcomes. Moreover, the AI-based approach to 3D imaging processing represents a modern extension of complex therapy of rare disease-related orofacial abnormalities, which are highly heterogeneous and often patient-specific. In this regard, school-age children and adolescents could start living much fuller lives comparable to their healthy peers. The patient with the Ellis-van Creveld syndrome (EvC; MIM #225500, ORPHA:289) in the form of a case report demonstrates respective treatment strategies during their long-term therapy.

The oculo-facio-cardio-dental syndrome (OFCD) is an ultra-rare multiple congenital anomaly. This report describes clinical findings emphasising dental phenotype in five, molecularly confirmed, female cases from two Czech families. Dental examinations were carried out. An orthopantomogram was taken in three patients, and all patients’ intraoral cavities and teeth were photographed. Exome sequencing was performed in both probands. Results were validated by Sanger DNA sequencing which was also used to follow segregation of the variants in first-degree relatives. Dental abnormalities and congenital cataracts were present in all five cases, whilst other signs were variable and included facial dysmorphism, microphthalmia, and cardiac and skeletal abnormalities. Two individuals had cleft lip and/or cleft palate. Radiculomegaly occurred in three patients with permanent teeth and was diagnosed on orthopantomograms. Two patients had agenesis of permanent teeth. Malocclusion was also present in two patients due to crowding and a Class III malocclusion and mandibular overjet. De novo novel pathogenic variants in the BCOR gene were identified; c.2382del p.(Lys795Argfs*12) and c.3914dup p.(Gln1306Alafs*20) and co-segregated with the disease in each family. The OFCD syndrome has a unique dental phenotype and dentists should be aware of signs of this ultra-rare genetic disorder. All patients with congenital cataracts and dental abnormalities, including those without a family history, should be referred for genetic testing and indicated to specialised dental care.

Cone beam computed tomography (CBCT) has emerged as a crucial radiographic technique for orthodontic diagnosis and treatment planning, particularly for cases requiring the assessment of complex anatomical relationships. In the first part of the study, we examined metric characteristics measured on 3D cranial models of patients before and after surgery. In the second part, we conducted more complex data processing, analyzing a set of 12 cranial feature points using Procrustes analysis to quantify and visually represent surgical modifications. The third part involved comparing 3D facial surfaces using Iterative Closest Point (ICP) alignment and nearest point-to-point distances. Additionally, we tested point configurations in the facial soft tissues. The study included a group of orthodontic patients from whom CBCT data and 3D facial scans were obtained during treatment. The results demonstrated that each method could assess preoperative and postoperative changes to varying degrees. They also highlighted potential gender differences in surgical modifications that warrant further investigation and consideration during surgical planning. The aim of our study was to compare 3D visualizations of skull and facial models before and after surgery, to assess the degree of relative agreement or similarity, and to identify any morphological differences.

BACKGROUND: The Roma population is a genetically isolated population with a shared origin, totaling between 10 to 14 million individuals worldwide, stemming from a limited number of "genetic founders". Roma individuals exhibit specific hereditary diseases, often stemming from recessive genetic variants due to a higher degree of consanguinity, with recent molecular-genetic investigations shedding light on several conditions prevalent within the Czech Roma population. However, an overview of stomatological issues in diagnosing such diseases proves challenging, leading to frequent underdiagnosis or misdiagnosis. METHODS: The contribution monitors the clinical description, typical symptoms and treatment options including dental abnormalities in rare genetic diseases in the Roma population which are treated in ERN CRANIO centre at Motol University Hospital in Prague. RESULTS: Our research provides examples of autosomal recessive diseases, which can be molecularly confirmed, and prevalent within the Roma community. These include congenital cataract syndrome, facial dysmorphism and demyelinating neuropathy, non-syndromic prelingual e.g. deafness with GJB2 gene impairment, and myasthenic syndrome. CONCLUSION: Our report aimed to provide a systematic review of dental phenotypes which can relate to CzechRoma's rare genetic disorders therapy including dental treatment. Understanding is important for preventing unterdiagnosis or treatment for the patients affected review of observed (Fig. 6, Ref. 27). Text in PDF www.elis.sk

The integration of computational intelligence and augmented reality has become increasingly prevalent in dental practices. Three-dimensional (3D) printing techniques have now become routine in orthodontics, prosthetics, and maxillofacial surgery. The objective of this study is to assess the effectiveness of stereolithography (SLA) printed models compared to traditional plaster casts over a three-year period. The experimental dataset consisted of 36 orthodontic patients, each with SLA printed models and plaster casts for both the upper and lower jaws, resulting in a total of 72 scans in the form of Standard Template Library (STL) files and 72 traditional impressions. The upper dental arch models were constructed using an SLA 3D printer, employing a blue 405 nm laser beam to solidify a liquid polymer. The classical plaster casts were prepared in a dental laboratory. The models were retained for long-term orthodontic therapy control. To evaluate the differences between the dental models, a laboratory scanner was used to generate virtual casts. The meshes obtained were adjusted and pre-aligned using the best-fit algorithm. Subsequently, registration of the models was performed using the iterative closest point (ICP) algorithm. Distances between the point clouds and meshes for each point of the printed model were calculated by determining the nearest triangle on the reference mesh (cast). Additionally, the model surfaces were assessed using a scanning electron microscope and a stereomicroscope. The results from 22 experimental datasets indicated a high level of agreement between the plaster casts and virtual surfaces, with a mean absolute difference of 0.018 mm and a standard deviation of 0.17 mm. These values were obtained by averaging 231,178 data points for each comparison. Overall, this study demonstrates the comparability and accuracy of SLA printed models in relation to traditional plaster casts, supporting their potential as reliable alternatives in dental practice.

The goal of study was to explore the role of 3D CBCT (cone beam computer tomography) in detecting impacted canines and their movement to evaluate the influence of orthodontic therapy parameters on treatment options, and to monitor quality of healing process based on shape and size of sinus maxillae volume. It is known that the volume of maxillary sinus plays an important role in patients with impacted teeth. The prospective study consisted of 26 individuals. For each individual, pre-treatment and post-treatment CBCT data were acquired. Changes of size, and position of impacted canine in 3D CBCT image before and after therapy were prepared using 3D reconstruction. Volumetric measurements of the maxillary sinuses were performed before and after orthodontic therapy of impacted canines, using InVivo6 software. The main effects MANOVA performed on linear measurements showed metric differences between pre-op and post-op images. A paired t -test showed no statistically significant differences between pre-op and post-op values of the sinus volume. Changes of size and position of impacted canine in 3D image before and after therapy were precise and reproducible, using 3D reconstruction in three planes – horizontal, midsagittal, and coronal. The linear measurements showed metric differences between pre-op and post-op images.