Arfana Mallah

Background: Over the past few decades, environmental pollution has appeared to be one of the most crucial global problems. The widespread intensification of numerous hazardous pollutants in the environment need the modern researchers to develop viable, reproducible and cost-effective determination tools for the reliable environmental analysis. The beneficial, as well as perilous, biological compounds are receiving growing interest due to their variable composition which produces advantageous and toxic impacts on human and the environment. Several conventional analytical methods have been established for the pharmaceutical and environmental analysis. However, certain drawbacks limited their practices in the modern rapidly growing era of science and technology. The development of electrochemical sensors has emerged as more beneficial and promising tool as against other traditional analytical approaches, in terms of simplicity, cost-effectiveness, sensitivity, stability and reliability. Nonetheless, the over potential and low anodic/cathodic current response are both considered as bottlenecks for the determination of electroactive entities exploiting electrochemical sensors. Interestingly, these problems can be easily resolved by modifying the electrodes with a variety of conductive materials, especially nanostructures.Objective: This review covers different electrochemical methods, reported in the literature, for the environmental and pharmaceutical analysis through simple and cost-effective nanostructures-based sensors. The electrochemical techniques with different modes and the modification of electrodes with highly conductive and prolific polymeric and nanostructured materials used for the determination of different environmental and pharmaceutical samples are the main prominence of this review. Various kinds of nanomaterials, e.g. metal, metal oxide and their composites, have been synthesized for the fabrication of sensitive electrodes.Conclusion: Nanostructures played a pivotal role in the modification of electrodes, which substantially enhanced the capability and sensitivity of electrochemical sensors. The proper modification of electrodes has materialized the swift detection of electroactive compounds at very low limits and offered the feasible determination procedure without any kind of signal fluctuation and over potential. In crux, due to their enhanced surface area and excellent catalytic properties, nanomaterials recently appeared as the most promising candidates in the field of electrode modification and significantly impacted the detection protocols for various environmental pollutants, viz. pesticides, metal ions and drugs.

It is always demanded to prepare a nanostructured material with prominent functional properties for the development of a new generation of devices. This study is focused on the synthesis of heart/dumbbell-like CuO nanostructures using a low-temperature aqueous chemical growth method with vitamin B₁₂ as a soft template and growth directing agent. CuO nanostructures are characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) techniques. CuO nanostructures are heart/dumbbell like in shape, exhibit high crystalline quality as demonstrated by XRD, and have no impurity as confirmed by XPS. Apparently, CuO material seems to be porous in structure, which can easily carry large amount of enzyme molecules, thus enhanced performance is shown for the determination of uric acid. The working linear range of the biosensor is 0.001 mM to 10 mM with a detection limit of 0.0005 mM and a sensitivity of 61.88 mV/decade. The presented uric acid biosensor is highly stable, repeatable, and reproducible. The analytical practicality of the proposed uric acid biosensor is also monitored. The fabrication methodology is inexpensive, simple, and scalable, which ensures the capitalization of the developed uric acid biosensor for commercialization. Also, CuO material can be used for various applications such as solar cells, lithium ion batteries, and supercapacitors.

The production of a nanomaterial with enhanced and desirable electrocatalytic properties is of prime importance, and the commercialization of devices containing these materials is a challenging task. In this study, unique cupric oxide (CuO) nanostructures were synthesized using lysine as a soft template for the evolution of morphology via a rapid and boiled hydrothermal method. The morphology and structure of the synthesized CuO nanomaterial were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively. The prepared CuO nanostructures showed high potential for use in the electrocatalytic oxidation of glucose in an alkaline medium. The proposed enzyme-free glucose sensor demonstrated a robust response to glucose with a wide linear range and high sensitivity, selectivity, stability, and reproducibility. To explore its practical feasibility, the glucose content of serum samples was successfully determined using the enzyme-free sensor. An analytical recovery method was used to measure the actual glucose from the serum samples, and the results were satisfactory. Moreover, the presented glucose sensor has high chemical stability and can be reused for repetitive measurements. This study introduces an enzyme-free glucose sensor as an alternative tool for clinical glucose quantification.

This paper reports the utilization of 2‐pyrrolecarboxaldehyde‐4‐phenylsemicarbazone (PPS) as a complexing reagent for the simultaneous determination and separation of Ni(II), Cu(II), Pd(II), and Ag(I) by reversed‐phase high‐performance liquid chromatography with UV detector. A good separation was achieved using Microsorb C18 column (150 × 4.6 mm i.d.) with a mobile phase consisted of methanol : acetonitrile : water : sodium acetate (1 mM) (68 : 6.5 : 25 : 0.5 v/v/v/v) at a flow rate of 1 mL/min. The detection was performed at 280 nm. The linear calibration range was 2–10 μg/mL for all metal ions. The detection limits (S/N = 3) were 80 pg/mL for Ni(II), 0.8 ng/mL for Cu(II), 0.16 ng/mL for Pd(II), and 0.8 ng/mL for Ag(I). The applicability and the accuracy of the developed method were estimated by the analysis of Ni(II) in hydrogenated oil (ghee) samples and Pd(II) in palladium charcoal.

Micellar electrokinetic chromatography (MEKC) was examined for the separation and determination of Mo(VI), Cr(VI), Ni(II), Pd(II), and Co(III) as diethyl dithiocarbamate (DDTC) chelates. The separation was achieved from fused silica capillary (52cm×75

This work studies the development of a simple and fairly rapid methodology for simultaneous determination/separation of three frequently co-administered drugs; ciprofloxacin (CIP), paracetamol (PCT) and diclofenac sodium (DIC) using capillary electrophoresis (CE) with UV detection at 260 nm. Separation was achieved in only 6.5 min with a simple buffer of sodium tetraborate (50 mM) at pH 9.0. The Parameters affecting the separation and detection were optimized. The calibration curves were linear in the range of 5-500 µg/mL for CIP, 5-250 µg/mL for PCT and 1-125 µg/mL for DIC sodium under the optimized conditions. The lower limit of detection (LOD) was found to be 1 µg/mL for CIP & PCT and 0.5 µg/mL for DIC. The method was successfully used for the analysis of drugs in commercial pharmaceutical formulations and simultaneously from patient's urine sample with RSD 0.5-2.4%. Results obtained with CE method are compared with standard HPLC procedure and were found in good agreement.