Fernando B. Guijon
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Fernando B. Guijon has a total of 4 co-authors. Here are the top co-authors by number of shared publications:
Km Greulich-Bode
1 shared publicationMaria Paraskevas
1 shared publicationPatricia Baker
1 shared publication- Sabine Mai
Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
1 shared publication
Latest Publications
- 1 October 2007
- Published by Wolters Kluwer Health in Journal of Lower Genital Tract Disease
- Vol. 11 (4) , 265-272
Premalignant Cervical Lesions Are Characterized by Dihydrofolate Reductase Gene Amplification and c-Myc Overexpression
The c-Myc oncoprotein deregulation is associated with overall genomic instability and locus-specific genomic instability involving the dihydrofolate reductase (DHFR) locus. This study analyzes c-Myc protein levels and the stability of the DHFR gene in cervical tissue biopsies. The stability of the DHFR gene was examined by fluorescence in situ hybridization (FISH). c-Myc protein levels were evaluated using quantitative fluorescent immunohistochemistry. Forty-four cervical tissue biopsies were analyzed and included 33 preinvasive cervical lesions identified by histology, 14 samples were cervical intraepithelial neoplasia (CIN) 1; 7 were CIN 2; and 12 were CIN 3. Eleven biopsies had negative histology. c-Myc protein levels were elevated in CIN 1, 2, and 3 (p = .02) biopsies. Concomitantly, DHFR gene amplification was detected in CIN 1, 2, and 3 (p = .0001). The degrees of DHFR gene amplification and of c-Myc protein levels were a measure of the progressive degree of the lesion.
- 1 October 2007
- Published by Wolters Kluwer Health in Journal of Lower Genital Tract Disease
- Vol. 11 (4) , 265-272
Premalignant Cervical Lesions Are Characterized by Dihydrofolate Reductase Gene Amplification and c-Myc Overexpression
The c-Myc oncoprotein deregulation is associated with overall genomic instability and locus-specific genomic instability involving the dihydrofolate reductase (DHFR) locus. This study analyzes c-Myc protein levels and the stability of the DHFR gene in cervical tissue biopsies. The stability of the DHFR gene was examined by fluorescence in situ hybridization (FISH). c-Myc protein levels were evaluated using quantitative fluorescent immunohistochemistry. Forty-four cervical tissue biopsies were analyzed and included 33 preinvasive cervical lesions identified by histology, 14 samples were cervical intraepithelial neoplasia (CIN) 1; 7 were CIN 2; and 12 were CIN 3. Eleven biopsies had negative histology. c-Myc protein levels were elevated in CIN 1, 2, and 3 (p = .02) biopsies. Concomitantly, DHFR gene amplification was detected in CIN 1, 2, and 3 (p = .0001). The degrees of DHFR gene amplification and of c-Myc protein levels were a measure of the progressive degree of the lesion.