C A Flemming
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
C A Flemming has a total of 4 co-authors. Here are the top co-authors by number of shared publications:
A A Arrage
Center for Environmental Biotechnology , University of Tennessee , Knoxville, TN, 37932, USA
1 shared publicationD C White
Center for Environmental Biotechnology , University of Tennessee , Knoxville, TN, 37932, USA ; Oak Ridge National Laboratory , Oak Ridge, TN, 37831, USA
1 shared publicationH C van der Mei
Laboratory for Materia Technica , University of Groningen , 9713 AV, Groningen, The Netherlands
1 shared publicationR J Palmer
1 shared publication
Latest Publications
- 1 December 1998
- Published by Taylor & Francis in Biofouling
- Vol. 13 (3) , 213-231
Cell surface physicochemistry alters biofilm development ofpseudomonas aeruginosalipopolysaccharide mutants
The hydrophobic and electrostatic characteristics of bacterial cell surfaces were compared with attachment proclivity and biomass accumulation over time between wildtype Pseudomonas aeruginosa serotype O6 (possesses A and B band LPS), and three LPS‐deficient mutants, viz. A28 (A+B‐), R5 (A+B‐), and Gt700 (A‐B‐). The hydrophobic character of each serotype was determined by hydrophobic interaction chromatography and salt‐aggregation, and strains were ranked similarly by each method, viz. R5 ≥ A28 > Gt700 > 06. The anionic characteristics of cell‐surfaces were determined by electrostatic interaction chromatography and by zeta‐potential measurements, and ranked R5 > A28 ≥ Gt700 > 06. Adhesion and biofilm accumulation on stainless steel were significantly different between strains, following the order R5 > A28 >> O6>Gt700. Biofilm rankings were similar on glass, a second hydrophilic substratum. The mutant strains with a strongly hydrophobic character (R5 and A28) demonstrated a significantly greater capacity to form biofilms. These adherent mutants also appeared to have a more anionic cell surface, which may have played a role in biofilm formation on the hydrophilic substrata.
- 1 December 1998
- Published by Taylor & Francis in Biofouling
- Vol. 13 (3) , 213-231
Cell surface physicochemistry alters biofilm development ofpseudomonas aeruginosalipopolysaccharide mutants
The hydrophobic and electrostatic characteristics of bacterial cell surfaces were compared with attachment proclivity and biomass accumulation over time between wildtype Pseudomonas aeruginosa serotype O6 (possesses A and B band LPS), and three LPS‐deficient mutants, viz. A28 (A+B‐), R5 (A+B‐), and Gt700 (A‐B‐). The hydrophobic character of each serotype was determined by hydrophobic interaction chromatography and salt‐aggregation, and strains were ranked similarly by each method, viz. R5 ≥ A28 > Gt700 > 06. The anionic characteristics of cell‐surfaces were determined by electrostatic interaction chromatography and by zeta‐potential measurements, and ranked R5 > A28 ≥ Gt700 > 06. Adhesion and biofilm accumulation on stainless steel were significantly different between strains, following the order R5 > A28 >> O6>Gt700. Biofilm rankings were similar on glass, a second hydrophilic substratum. The mutant strains with a strongly hydrophobic character (R5 and A28) demonstrated a significantly greater capacity to form biofilms. These adherent mutants also appeared to have a more anionic cell surface, which may have played a role in biofilm formation on the hydrophilic substrata.