Donato Casamassima

Lippia citriodora is a plant traditionally used for its anti-inflammatory, antioxidant and antispasmodic effects, as well as for additional biological activities proven in cell culture, animal studies and a small number of human clinical trials. The plant has also shown a marked improvement in blood lipid profile in some animal species. In the present preliminary study, we investigated the effect of a leaf extract on lipid and oxidative blood profile of hypercholesterolemic volunteers. Twelve adults received Lippia citriodora extract caps, containing 23% phenylpropanoids, (100 mg, once a day) for 16 weeks. Selected blood lipids and plasma oxidative markers were measured at baseline and after 4, 8 and 16 weeks of treatment. Compared with baseline, total cholesterol levels significantly decreased and high-density lipoprotein cholesterol increased, while low-density lipoprotein cholesterol and triglycerides showed only a downward trend. Oxidative status was improved due to a decrease in the concentration of total oxidant status, reactive oxygen metabolites and malondialdehyde, and a significant increase in ferric reducing ability of plasma, vitamin A and vitamin E. These preliminary results suggest that dietary supplementation with Lippia citriodora extract can improve the lipid profile, enhance blood antioxidant power, and could be a valuable natural compound for the management of human hypercholesterolemia.

The objective of the present study was to evaluate the effects of dam and offspring dietary supplementation with a natural feed additive on the growth performance and meat quality parameters of growing rabbits. The growing rabbits are selected from lactating does receiving a control diet (C) or diets supplemented with 0.3% (SP1) and 0.6% (SP2) of feed additive containing brown seaweeds (Laminaria spp.) and plant extracts. In the postweaning phase, the growing rabbits remained in the treatment group defined by their does and the trial lasted 42 days. The average daily feed intake and feed conversion ratio were improved in the rabbit fed 0.6% of the natural feed additive. The cholesterol content tended to be lower in Longissimus lumborum (LL) muscle and decrease in Semimembranosus (SM) muscle (in SP2 −41.36% than controls). The α tocopherol and retinol content were enhanced in both muscles of rabbit fed the natural mixture (SP1 and SP2 groups). An improvement of sensory attributes of texture was observed in both muscles from rabbit fed natural mixture. In conclusion, long term supplementation of both lactating does and offspring with the high dosage of brown seaweed and plant polyphenols improves growth performance and enhances meat nutritional and sensory parameters.

Polyphenol-rich foods could have a pivotal function in the prevention of oxidative stress-based pathologies and antibacterial action. The purpose of this study was to investigate the in vitro antimicrobial activity, as well as the in vitro and In Vivo antioxidant capacities of wild Prunus spinosa L. fruit (PSF) from the southeast regions of Italy. The total phenolic content (TPC) was quantified, and the single polyphenols were analyzed by HPLC-DAD, showing high rutin and 4-hydroxybenzoic acid levels, followed by gallic and trans-sinapic acids. PSF extract demonstrated antimicrobial activity against some potentially pathogenic Gram-negative and Gram-positive bacteria. Besides, we investigated the cellular antioxidant activity (CAA) and the hemolysis inhibition of PSF extract on human erythrocytes, evidencing both a good antioxidant power and a marked hemolysis inhibition. Furthermore, an In Vivo experiment with oxidative stress-induced rats treated with a high-fat diet (HFD) and a low dose of streptozotocin (STZ) demonstrated that PSF has a dose-dependent antioxidant capacity both in liver and in brain. In conclusion, the wild Italian Prunus spinosa L. fruit could be considered a potentially useful material for both nutraceutical and food industries because of its antioxidant and antimicrobial effects.

The effects of two doses of a verbascoside-based dietary supplement on performance, some blood parameters, and plasma oxidative status in Lacaune suckling lambs were investigated. Twenty-four lambs were divided into three groups of eight animals each and fed per os a dietary supplement with a...

The trial, lasted 7 months and was performed on 60 lactating autochthonous goat populations (Montefalcone and Valfortorina) extensively reared in Molise Region. Goat milk yield and composition, and pasture chemical and floristic composition were recorded monthly. At the beginning (30d), at half (105d) and at the end (210d) of trial body weight and body condition score (BCS) were recorded; besides jugular blood samples were taken at 30, 105 e 210 d to determined the following parameters: glucose, total protein, albumin, urea, triglycerides, total and HDL cholesterol, NEFA, calcium, magnesium, sodium and potassium. The two populations showed a milk yield of 275 kg e 258 kg (Montefalcone e Valfortorina goat, respectively) throughout 180 days of lactation, which was similar or higher than mean milk yield of other goat breeds (Sarda, Ionica, Argentata dell’Etna). BCS increased significantly (P<0.01) during lactation in both populations. Blood parameters displayed significant changes (P<0.01) during the trial in Montefalcone goat for glucose, albumin, urea, triglycerides, total and HDL cholesterol, NEFA, sodium and potassium instead Valfortorina goat showed significant differences in total protein, urea, total and HDL cholesterol, NEFA, magnesium, sodium (P<0.01) and albumin and potassium (P<0.05). In both population, milk yield and quality showed promising results for autochthonous goats preservation.

Milk quality in autochthonous goat extensively reared in Molise: study of some bionutritional components during lactation