Zhaohui Gong

Most previous studies have examined medical postgraduate research training from a single dimension, lacking a full-chain analysis that integrates capability demand, actual possession, obstacles, and output. Consequently, the measurement of capability gaps and the analysis of underlying training model deficiencies remain insufficient. To address this gap, we administered a self-designed multidimensional questionnaire to 86 clinical medicine postgraduates at a medical school, covering research cognition, interest, capability demand and possession, participation pathways, difficulties, and outputs. The aim was to systematically characterize the current situation, identify problems, and propose optimization strategies. Over 90% of participants expressed interest in research, yet only 1.16% self-rated as very knowledgeable. The largest demand-possess gap was for writing and publication (86.05% vs. 16.28%), followed by independent research capability (75.58% vs. 11.63%). A total of 59.30% cited lack of foundational knowledge, making experiments very difficult, as the greatest challenge, and 66.28% had no research achievements. The primary source of research topics was supervisor assignment (54.65%), with only 4.65% choosing topics independently. No statistically significant differences were found across grades or training types (P > 0.05). These findings reveal a structural high demand, low possession gap in medical postgraduate research training, with early research experience deficit and a passive research model as key constraining factors. Accordingly, an integrated bachelor-postgraduate progressive research competency training system is proposed.

Background: Non-coding RNAs (ncRNAs) are transcribed RNA molecules that do not encode proteins but regulate diverse biological processes. Dysregulation of ncRNAs is implicated in cancer, where chemical modifications such as N6-methyladenosine (m6A), N4-acetylcytidine (ac4C), and glycosylation critically influence their function. However, these modifications, as precise regulators of ncRNA activity, have been less well-documented and understood in tumorigenesis and cancer progression. Methods: This article systematically analyzes the roles of chemically modified ncRNAs – ribosomal RNA (rRNA), circular RNA (circRNA) and others – in cancer biology, synthesizingevidence from published studies on their mechanistic involvement in malignancy. Results: We reveal how specific chemical modifications drive oncogenesis, impact cancer diagnosis, and affect therapeutic responses, while also exploring their prognostic potential. Furthermore, we highlight emerging connections between ncRNA epitranscriptomics and cancer. Conclusions: This review provides novel insights into ncRNA epitranscriptomics as emerging biomarkers and intervention targets for precision oncology.

Engineered circular RNAs (circRNAs) are emerging as promising platforms for RNA-based vaccines in cancer treatment. We summarize the recent advances of design, synthesis, and delivery of circRNA-based cancer vaccines, and highlight the applications and challenges of circRNA vaccines in cancer therapy. Further enhancements are required in areas such as antigen selection, targeted delivery, multidimensional crosstalks, and clinical trial assessments to advance the efficacy and safety of circRNA vaccines in cancer.

[This corrects the article on p. 2797 in vol. 9, PMID: 31911863.].

Scientific research training (SRT) is crucial for fostering skills and innovation in college students. However, the status of SRT in medical undergraduates and postgraduates, as well as the impact of early SRT on students’ innovation abilities, remain largely unknown. This study assessed the role of SRT in culturing innovation abilities among medical students. The results revealed that undergraduates exhibited a greater understanding of scientific research compared to postgraduates. Notably, undergraduates demonstrated a higher level of understanding of SRT in contrast to postgraduates (81.26% vs. 56.97%). A significant proportion (>70%) of undergraduates had engaged in research activities early in their academic journey, with an average participation duration exceeding 1 year. Moreover, undergraduates initiated their involvement in SRT during the first- and second- year of undergraduates, which was earlier than postgraduates. Personal interest emerged as a primary motivator for engaging in SRT, with postgraduates exhibiting higher research aspirations and achieving greater research outcomes. Postgraduates also received more substantial guidance from advisors, leading to enhanced academic performance compared to undergraduates (90.7% vs. 67.59%). Interestingly, students expressed satisfaction with laboratory conditions and attributed their academic success to personal efforts. These findings underscore the importance of early SRT in cultivating the innovation abilities of medical students.

Non-small cell lung cancer (NSCLC) is typically diagnosed at advanced stages, which limits the effectiveness of therapeutic interventions. The present study aimed to explore the role of the newly identified circLIFRSA in the PTEN/AKT signaling pathway and its involvement in the malignant processes of NSCLC. CircLIFRSA expression was identified through microarray analysis, and its levels in NSCLC samples were quantified by RT-qPCR. The impact of circLIFRSA on cell growth, proliferation, apoptosis, and cell cycle were evaluated by MTT assay, colony formation assay, and flow cytometry. Additionally, Western blotting was employed to analyze the expression of PTEN and phosphorylated AKT (pAKT) in NSCLC cells. The expression of circLIFRSA was found to be significantly reduced in NSCLC cells and tissues. This downregulation correlated with various clinicopathological characteristics and indicated its potential as an early diagnostic biomarker for NSCLC. Importantly, circLIFRSA was shown to inhibit cell growth and proliferation while promoting apoptosis in NSCLC cells. Mechanically, circLIFRSA was found to attenuate the malignant processes of NSCLC cells via the miR-1305/PTEN axis and the suppression of AKT phosphorylation. These findings indicate that circLIFRSA/miR-1305/PTEN axis attenuates malignant processes by regulating AKT phosphorylation, and provide new insights into the potential of circLIFRSA as a biomarker for early diagnosis and as a promising therapeutic target in NSCLC.

Background Scientific research training (SRT) in basic medical sciences and clinical sciences plays critical roles in the development of clinical skills and innovation ability. We aimed to explore the current status of SRT in both medical undergraduates and postgraduates, and the relationship between early SRT and innovation ability in medical students. Methods We designed two questionnaires for undergraduates and postgraduates respectively to investigate the understanding, and participation of SRT, the motivations and factors for engaging in research, the advisor’s guidance of SRT, the academic performance of SRT, and the assessment of research conditions and suggestions for SRT. The questionnaire survey was conducted on 108 undergraduates and 86 postgraduates at Ningbo University. Then we collected data and analysed it with descriptive statistics. Results The results showed that undergraduates had more interest, understanding, and requirements for scientific research. Understanding of SRT in undergraduates (81.26%) was higher than that in postgraduates (56.97%). More than 70% of undergraduates had research experience in the early grades and the average time participating in SRT was more than one year. Participating in SRT in undergraduates (first and second year of undergraduate) was earlier than that in postgraduates (first year of postgraduate). In particular, personal interest was the major motivation for engaging in SRT. The vast majority of postgraduates (89.53%) had higher research goals than undergraduates (75%). These specific goals led to much higher research gains. Additionally, postgraduates (90.7%) could receive more higher proportion of guidance than undergraduates (67.59%), and thus higher academic performance (15.12% vs. 2.78%). Interestingly, most students were satisfied with the lab conditions and their personal efforts were primarily to achieve better academic performance. Conclusions These findings indicated that early SRT is strongly linked to the innovation ability of medical students, suggesting a necessity to construct an early extracurricular SRT program to train more future clinician-scientists and to improve the quality of medical education.

[This corrects the article on p. 2797 in vol. 9, PMID: 31911863.].

Introduction: Circular RNAs (circRNAs) are dysregulated in cancers and are stably expressed in body fluids such as blood. We therefore identified and evaluated the clinical value of a newly found circRNA VPS35L (circVPS35L) as a biomarker for the diagnosis of non-small cell lung cancer (NSCLC). Methods: Reverse-transcription quantitative PCR (RT-qPCR) was used to determine the expression levels of circVPS35L in tissues, whole blood, and cell lines. The actinomycin D assay and RNase R treatment were utilized to determine the stability of circVPS35L. Receiver operating characteristic (ROC) curve analysis was conducted to predict the diagnostic value of blood-derived circVPS35L in NSCLC. Results: CircVPS35L was found to be downregulated in NSCLC tissues and cell lines. Interestingly, circVPS35L expression was significantly correlated with tumor size (p = 0.0269), histology type (p < 0.0001), and TNM stage (p = 0.0437). Importantly, circVPS35L was poorly expressed in peripheral blood of NSCLC patients when compared with healthy controls and patients with benign lung disease. ROC analysis revealed a higher diagnostic value of circVPS35L than the three conventional tumor markers (CYFR21-1, NSE, and CEA) in patients with NSCLC. Moreover, circVPS35L was highly stable in peripheral blood when exposed to undesirable conditions. Conclusion: These findings demonstrate that circVPS35L has great potential as a novel biomarker for the diagnosis of NSCLC and can be used to distinguish NSCLC from benign lung disease.