Boris Kuehl
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Boris Kuehl has a total of 13 co-authors. Here are the top co-authors by number of shared publications:
- Annika Ricarda Völp
Thermo Fisher Scientific Karlsruhe Germany
1 shared publication - Burkhard Luy
Karlsruhe Institute of Technology (Karlsruhe, Germany)
1 shared publication - Christoph Syldatk
KIT - Karlsruhe Institute of Technology
1 shared publication - Claudia Muhle-Goll
Institute for Biological Interfaces 4 (IBG‐4) Karlsruhe Institute of Technology (KIT) Kaiserstrasse 12 Karlsruhe DE Germany
1 shared publication - Frank Kirschhöfer
Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
1 shared publication - Gerald Brenner-Weiss
Institute of Functional Interfaces, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany
1 shared publication
Latest Publications
- Open Access
- 23 December 2015
- Published by Springer Nature in AMB Express
- Vol. 5 (1) , 1-11
Glycolipids produced by Rouxiella sp. DSM 100043 and isolation of the biosurfactants via foam-fractionation
Microorganisms produce a great variety of secondary metabolites that feature surface active and bioactive properties. Those possessing an amphiphilc molecular structure are also termed biosurfactant and are of great interest due to their often unique properties. Rouxiella sp. DSM 100043 is a gram negative enterobacter isolated from peat-bog soil and described as a new biosurfactant producing species in this study. Rouxiella sp. produces glycolipids, biosurfactants with a carbohydrate moiety in its structure. This study characterizes the composition of glycolipids with different hydrophobicities that have been produced during cultivation in a bioreactor and been extracted and purified from separated foam. Using two dimensional nuclear magnetic resonance spectroscopy, the hydrophilic moieties are elucidated as glucose with various acylation sites and as talose within the most polar glycolipids. The presence of 3′ hydroxy lauroleic acid as well as myristic and myristoleic acid has been detected.
- 23 December 2015
- Published by Springer Nature in AMB Express
- Vol. 5 (1) , 1-11
Open AccessGlycolipids produced by Rouxiella sp. DSM 100043 and isolation of the biosurfactants via foam-fractionation
Microorganisms produce a great variety of secondary metabolites that feature surface active and bioactive properties. Those possessing an amphiphilc molecular structure are also termed biosurfactant and are of great interest due to their often unique properties. Rouxiella sp. DSM 100043 is a gram negative enterobacter isolated from peat-bog soil and described as a new biosurfactant producing species in this study. Rouxiella sp. produces glycolipids, biosurfactants with a carbohydrate moiety in its structure. This study characterizes the composition of glycolipids with different hydrophobicities that have been produced during cultivation in a bioreactor and been extracted and purified from separated foam. Using two dimensional nuclear magnetic resonance spectroscopy, the hydrophilic moieties are elucidated as glucose with various acylation sites and as talose within the most polar glycolipids. The presence of 3′ hydroxy lauroleic acid as well as myristic and myristoleic acid has been detected.
- 17 July 2011
- Published by Springer Nature in Analytical and Bioanalytical Chemistry
- Vol. 401 (5) , 1593-1600
MALDI-ToF mass spectrometry–multivariate data analysis as a tool for classification of reactivation and non-culturable states of bacteria
Some bacterial life states are only difficult to describe and to detect because they are on the border of active metabolism. A prominent example is the so-called viable but non-culturable state, which is mainly characterized by the inability of bacteria to grow on synthetic media. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF/MS) in combination with multivariate data analysis represents a powerful tool for mass-spectrometric pattern recognition of biological samples. This method is already used for differentiation of bacterial strains. In this study we present a rapid readout method based on MALDI-ToF/MS in combination with principal component analysis to classify the bacterial non-culturable state using Enterococcus faecalis as a model organism. By applying this technique to samples of different physiological states, distinct clusters were calculated and all mass spectra were classified correctly into groups of similar type concerning their physiological state.
- 17 July 2011
- Published by Springer Nature in Analytical and Bioanalytical Chemistry
- Vol. 401 (5) , 1593-1600
MALDI-ToF mass spectrometry–multivariate data analysis as a tool for classification of reactivation and non-culturable states of bacteria
Some bacterial life states are only difficult to describe and to detect because they are on the border of active metabolism. A prominent example is the so-called viable but non-culturable state, which is mainly characterized by the inability of bacteria to grow on synthetic media. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF/MS) in combination with multivariate data analysis represents a powerful tool for mass-spectrometric pattern recognition of biological samples. This method is already used for differentiation of bacterial strains. In this study we present a rapid readout method based on MALDI-ToF/MS in combination with principal component analysis to classify the bacterial non-culturable state using Enterococcus faecalis as a model organism. By applying this technique to samples of different physiological states, distinct clusters were calculated and all mass spectra were classified correctly into groups of similar type concerning their physiological state.