Gaurav V. Sanghvi
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Gaurav V. Sanghvi has a total of 2 co-authors. Here are the top co-authors by number of shared publications:
- Kishore Shankarsinh Rajput
Department of Botany, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, India
1 shared publication - Rina D. Koyani
Department of Botany, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390002, India
1 shared publication
Latest Publications
- 28 May 2011
- Published by Korean Society for Microbiology and Biotechnology in Journal of Microbiology and Biotechnology
- Vol. 21 (5) , 470-176
Isolation, Optimization, and Partial Purification of Amylase from Chrysosporium asperatum by Submerged Fermentation
A potent fungus for amylase production, Chrysosporium asperatum, was isolated from among 30 different cultures obtained from wood samples collected in the Junagadh forest, India. All of the isolated cultures were screened for their ability to produce amylase by submerged fermentation. Among the selected cultures, C. asperatum (Class Euascomycetes; Onygenales; Onygenaceae) gave maximum amylase production. In all of the different media tested, potato starch was found to be a good substrate for production of amylase enzyme at 30 degrees C and pH 5.0. Production of enzyme reached the maximum when a combination of starch and 2% xylose, and organic nitrogen (1% yeast extract) and ammonium sulfate were used as carbon and nitrogen sources, respectively. There was no significant effect of metal ions on enzyme activity. The enzyme was relatively stable at 50 degrees C for 20 min, and no inhibitory effect of Ca+2 ions on amylase production was observed.
- 28 May 2011
- Published by Korean Society for Microbiology and Biotechnology in Journal of Microbiology and Biotechnology
- Vol. 21 (5) , 470-176
Isolation, Optimization, and Partial Purification of Amylase from Chrysosporium asperatum by Submerged Fermentation
A potent fungus for amylase production, Chrysosporium asperatum, was isolated from among 30 different cultures obtained from wood samples collected in the Junagadh forest, India. All of the isolated cultures were screened for their ability to produce amylase by submerged fermentation. Among the selected cultures, C. asperatum (Class Euascomycetes; Onygenales; Onygenaceae) gave maximum amylase production. In all of the different media tested, potato starch was found to be a good substrate for production of amylase enzyme at 30 degrees C and pH 5.0. Production of enzyme reached the maximum when a combination of starch and 2% xylose, and organic nitrogen (1% yeast extract) and ammonium sulfate were used as carbon and nitrogen sources, respectively. There was no significant effect of metal ions on enzyme activity. The enzyme was relatively stable at 50 degrees C for 20 min, and no inhibitory effect of Ca+2 ions on amylase production was observed.
- Open Access
- 29 April 2010
- Published by Taylor & Francis in Mycology
- Vol. 1 (2) , 106-112
Thermostable xylanase production and partial purification by solid-state fermentation using agricultural waste wheat straw
A locally isolated strain of Trichoderma harzianum was studied for production of xylanase (EC 3.2.1.8) using lignocellulosic substrates for solid-state fermentation. Among the different substrates used, wheat straw produced the highest yields (146 IU/ml). The influence of temperature, pH, moistening agents, moisture level, carbon sources, nitrogen sources, pretreatments and metal ions were evaluated with respect to xylanase production. Highest xylanase production was obtained using wheat straw after 12 days of incubation. Different substrate combination ratios and the effect of particle size were also checked against xylanase production. Maximum xylanase production was observed in a wheat straw/rice straw ratio of 1:1, with a particle size of 0.45–0.5 mm. Media supplementation with xylose as a carbon source in a ratio 1:5 (carbon source/substrate) gave maximum activity (157 IU/ml). Nitrogen supplementations from yeast extract produced xylanase activity of 141 IU/ml. Optimum xylanase production was observed at 30°C and pH 5.0. Xylanase production was enhanced in the presence of Ca2+ and Zn2+ ions. Ammonium sulfate fractionation (20–80% saturation) of partially purified xylanase yielded 76.5% of the enzyme with a 3.53-fold purification. The molecular weight of xylanase was found to be ca. 29,000 Da by SDS–PAGE.
- 29 April 2010
- Published by Taylor & Francis in Mycology
- Vol. 1 (2) , 106-112
Open AccessThermostable xylanase production and partial purification by solid-state fermentation using agricultural waste wheat straw
A locally isolated strain of Trichoderma harzianum was studied for production of xylanase (EC 3.2.1.8) using lignocellulosic substrates for solid-state fermentation. Among the different substrates used, wheat straw produced the highest yields (146 IU/ml). The influence of temperature, pH, moistening agents, moisture level, carbon sources, nitrogen sources, pretreatments and metal ions were evaluated with respect to xylanase production. Highest xylanase production was obtained using wheat straw after 12 days of incubation. Different substrate combination ratios and the effect of particle size were also checked against xylanase production. Maximum xylanase production was observed in a wheat straw/rice straw ratio of 1:1, with a particle size of 0.45–0.5 mm. Media supplementation with xylose as a carbon source in a ratio 1:5 (carbon source/substrate) gave maximum activity (157 IU/ml). Nitrogen supplementations from yeast extract produced xylanase activity of 141 IU/ml. Optimum xylanase production was observed at 30°C and pH 5.0. Xylanase production was enhanced in the presence of Ca2+ and Zn2+ ions. Ammonium sulfate fractionation (20–80% saturation) of partially purified xylanase yielded 76.5% of the enzyme with a 3.53-fold purification. The molecular weight of xylanase was found to be ca. 29,000 Da by SDS–PAGE.