Thierry Cl Laurent
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Thierry Cl Laurent has a total of 12 co-authors. Here are the top co-authors by number of shared publications:
- Alfarazdeg A Saad
Faculty of Medicine and Soba Teaching Hospital, University of Khartoum, Khartoum, Sudan
1 shared publication - Christophe Lambert
GSK, Rixensart, Belgium
1 shared publication - Claire M Mugasa
Koninklijk Instituut voor de Tropen (KIT)/Royal Tropical Institute, KIT Biomedical Research, Amsterdam, the Netherlands.
1 shared publication - Frank L Basiye
Kenya Medical Research Institute (KEMRI), Nairobi, Kenya
1 shared publication - Gerard J. Schoone
Royal Tropical Institute (KIT), Department of Biomedical Research, Parasitology Unit, Meibergdreef 39, Amsterdam 1105 AZ, The Netherlands
1 shared publication - Henk Dfh Schallig
Royal Tropical Institute (KIT), Department of Biomedical Research, Parasitology Unit, Meibergdreef 39, Amsterdam 1105 AZ, The Netherlands
1 shared publication
Latest Publications
- Open Access
- 2 March 2010
- Published by Springer Nature in Parasites & Vectors
- Vol. 3 (1) , 13
Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis
Background: Molecular methods to detect Leishmania parasites are considered specific and sensitive, but often not applied in endemic areas of developing countries due to technical complexity. In the present study isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of leishmaniasis. NASBA-OC, detecting Leishmania RNA, was evaluated using clinical samples from visceral leishmaniasis patients from East Africa (n = 30) and cutaneous leishmaniasis from South America (n = 70) and appropriate control samples. Results: Analytical sensitivity was 10 parasites/ml of spiked blood, and 1 parasite/ml of culture. Diagnostic sensitivity of NASBA-OC was 93.3% (95% CI: 76.5%-98.8%) and specificity was 100% (95% CI: 91.1%-100%) on blood samples, while sensitivity and specificity on skin biopsy samples was 98.6% (95% CI: 91.2%-99.9%) and 100% (95% CI: 46.3%-100%), respectively. Conclusion: The NASBA-OC format brings implementation of molecular diagnosis of leishmaniasis in resource poor countries one step closer.
- 2 March 2010
- Published by Springer Nature in Parasites & Vectors
- Vol. 3 (1) , 13
Open AccessSimplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis
Background: Molecular methods to detect Leishmania parasites are considered specific and sensitive, but often not applied in endemic areas of developing countries due to technical complexity. In the present study isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of leishmaniasis. NASBA-OC, detecting Leishmania RNA, was evaluated using clinical samples from visceral leishmaniasis patients from East Africa (n = 30) and cutaneous leishmaniasis from South America (n = 70) and appropriate control samples. Results: Analytical sensitivity was 10 parasites/ml of spiked blood, and 1 parasite/ml of culture. Diagnostic sensitivity of NASBA-OC was 93.3% (95% CI: 76.5%-98.8%) and specificity was 100% (95% CI: 91.1%-100%) on blood samples, while sensitivity and specificity on skin biopsy samples was 98.6% (95% CI: 91.2%-99.9%) and 100% (95% CI: 46.3%-100%), respectively. Conclusion: The NASBA-OC format brings implementation of molecular diagnosis of leishmaniasis in resource poor countries one step closer.
- 1 March 2004
- Published by Elsevier in Molecular Immunology
- Vol. 40 (17) , 1237-1247
Functional, molecular and structural characterisation of five anti-Brucella LPS mAb
- 1 March 2004
- Published by Elsevier in Molecular Immunology
- Vol. 40 (17) , 1237-1247
Functional, molecular and structural characterisation of five anti-Brucella LPS mAb