Michael A. Johnson
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Michael A. Johnson has a total of 5 co-authors. Here are the top co-authors by number of shared publications:
Scott G. Tyack
CSIRO Australian Animal Health Laboratory , Victoria , Australia
2 shared publicationsCatherine Pooley
CSIRO Livestock Industries, Private Bag 24, Geelong, Vic. 3220, Australia
1 shared publicationJagoda Ignjatovic
CSIRO Livestock Industries, Private Bag 24, Geelong, Vic. 3220, Australia
1 shared publication- Sameer A. Greenall
Centre for Cancer Research, Hudson Institute of Medical Research, and Department of Molecular and Translational Science, Monash University, 27-31 Wright Street, Clayton, Victoria 3168, Australia
1 shared publication Sandra I. Sapats
CSIRO Australian Animal Health Laboratory , Victoria , Australia
1 shared publication
Latest Publications
- Open Access
- 15 October 2010
- Published by Taylor & Francis in Avian Pathology
- Vol. 39 (5) , 339-348
Antibody fragments, expressed by a fowl adenovirus vector, are able to neutralize infectious bursal disease virus
Single-chain variable fragments (scFv) contain the heavy and light chain variable domains of immunoglobulin, joined by a short peptide linker. Previously, our laboratory has produced neutralizing scFv to epitopes of infectious bursal disease virus (IBDV). The in vitro delivery and expression of one of these scFv with and without the CH2-CH4 Fc domain of chicken IgY attached (scFv-Fc) by a serotype 8 fowl adenovirus (FAdV-8) vector was investigated in the present study. A panel of FAdV-8 vectors was constructed, each containing a different transgene (scFv or scFv-Fc), a different promoter to drive scFv and scFv-Fc transcription (CMVie or the fowl adenovirus major late promoter), and a different sized, right-hand end genomic deletion (52 bp or 2.3 kb). This panel was used to establish what effect these variables had on protein production, viral replication and scFv transcription, as measured by enzyme-linked imunosorbent assay and real-time polymerase chain reaction. Our results showed that, using a FAdV-8 vector containing the optimal CMVie promoter/2.3 kb deletion combination, we successfully expressed a secreted form of both scFv and scFv-Fc that were able to neutralize IBDV both in vitro and in ovo. These studies indicate that the FAdV-8 vector may be a promising candidate to deliver and express therapeutic molecules such as scFv and scFv-Fc in vivo in poultry.
- 15 October 2010
- Published by Taylor & Francis in Avian Pathology
- Vol. 39 (5) , 339-348
Open AccessAntibody fragments, expressed by a fowl adenovirus vector, are able to neutralize infectious bursal disease virus
Single-chain variable fragments (scFv) contain the heavy and light chain variable domains of immunoglobulin, joined by a short peptide linker. Previously, our laboratory has produced neutralizing scFv to epitopes of infectious bursal disease virus (IBDV). The in vitro delivery and expression of one of these scFv with and without the CH2-CH4 Fc domain of chicken IgY attached (scFv-Fc) by a serotype 8 fowl adenovirus (FAdV-8) vector was investigated in the present study. A panel of FAdV-8 vectors was constructed, each containing a different transgene (scFv or scFv-Fc), a different promoter to drive scFv and scFv-Fc transcription (CMVie or the fowl adenovirus major late promoter), and a different sized, right-hand end genomic deletion (52 bp or 2.3 kb). This panel was used to establish what effect these variables had on protein production, viral replication and scFv transcription, as measured by enzyme-linked imunosorbent assay and real-time polymerase chain reaction. Our results showed that, using a FAdV-8 vector containing the optimal CMVie promoter/2.3 kb deletion combination, we successfully expressed a secreted form of both scFv and scFv-Fc that were able to neutralize IBDV both in vitro and in ovo. These studies indicate that the FAdV-8 vector may be a promising candidate to deliver and express therapeutic molecules such as scFv and scFv-Fc in vivo in poultry.