Ping Guo
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Previous Organizations / Institutes
The organizations / institutes from the scholar's other articles in the past ten years
Research Subjects
Ping Guo has a total of 17 co-authors. Here are the top co-authors by number of shared publications:
- George K. Gittes
Division of Pediatric Surgery, UPMC Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
3 shared publications - Xiangwei Xiao
Department of Surgery, Children’s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, USA
3 shared publications - Yousef El-Gohary
Division of Pediatric General, Thoracic and Fetal Surgery, Chidren's Hospital Univeristy of Missouri, Columbia, Missouri, USA
3 shared publications Chiyo Shiota
Division of Pediatric General and Thoracic Surgery, Children’s Hospital of Pittsburgh, University of Pittsburgh Medical Center, One Children Hospital Drive, Pittsburgh, PA, USA
2 shared publicationsJohn Wiersch
Division of Pediatric General and Thoracic Surgery, Children’s Hospital of Pittsburgh, University of Pittsburgh Medical Center, One Children Hospital Drive, Pittsburgh, PA, USA
2 shared publicationsKrishna Prasadan
Division of Pediatric General and Thoracic Surgery, Children’s Hospital of Pittsburgh, University of Pittsburgh Medical Center, One Children Hospital Drive, Pittsburgh, PA, USA
2 shared publications
Latest Publications
- 1 November 2013
- Published by Elsevier in Laboratory Investigation
- Vol. 93 (11) , 1241-1253
Specific transduction and labeling of pancreatic ducts by targeted recombinant viral infusion into mouse pancreatic ducts
- 1 November 2013
- Published by Elsevier in Laboratory Investigation
- Vol. 93 (11) , 1241-1253
Specific transduction and labeling of pancreatic ducts by targeted recombinant viral infusion into mouse pancreatic ducts
- Open Access
- 1 March 2013
- Published by Taylor & Francis in Bioengineered
- Vol. 4 (2) , 103-106
A simplified purification method for AAV variant by polyethylene glycol aqueous two-phase partitioning
Adeno-Associated Virus (AAV) has been widely used for in vivo study and preclinical therapy due to its ability to mediate long-term transgene expression, its lack of pathogenicity and low immunogenicity. It has been found that AAV has more than ten serotypes, which each transfect certain types of cells in the viral infected organ. Current methods for purification of different AAV serotypes utilize CsCl or Iodixanol ultrahigh speed density gradient centrifugation, which is expensive and time consuming. We recently developed a simplified method, PEG/(NH4)2SO4 aqueous two phase partitioning, for purification of AAV serotype 2 and 8. The method does not require ultrahigh speed gradient centrifugation or chromatography. Here we further explore the simplified method for purification of other serotypes of AAV, serotype 6 and 9. This simplified method not only can be used to purify serotype 2 and 8, but also serotype 6 and 9, indicating that a variety of AAV serotypes can be purified by this method.
- 1 March 2013
- Published by Taylor & Francis in Bioengineered
- Vol. 4 (2) , 103-106
Open AccessA simplified purification method for AAV variant by polyethylene glycol aqueous two-phase partitioning
Adeno-Associated Virus (AAV) has been widely used for in vivo study and preclinical therapy due to its ability to mediate long-term transgene expression, its lack of pathogenicity and low immunogenicity. It has been found that AAV has more than ten serotypes, which each transfect certain types of cells in the viral infected organ. Current methods for purification of different AAV serotypes utilize CsCl or Iodixanol ultrahigh speed density gradient centrifugation, which is expensive and time consuming. We recently developed a simplified method, PEG/(NH4)2SO4 aqueous two phase partitioning, for purification of AAV serotype 2 and 8. The method does not require ultrahigh speed gradient centrifugation or chromatography. Here we further explore the simplified method for purification of other serotypes of AAV, serotype 6 and 9. This simplified method not only can be used to purify serotype 2 and 8, but also serotype 6 and 9, indicating that a variety of AAV serotypes can be purified by this method.
- 1 August 2012
- Published by Elsevier in Journal of Virological Methods
- Vol. 183 (2) , 139-146
Rapid and simplified purification of recombinant adeno-associated virus
- 1 August 2012
- Published by Elsevier in Journal of Virological Methods
- Vol. 183 (2) , 139-146
Rapid and simplified purification of recombinant adeno-associated virus