Akira Chiba
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Akira Chiba has a total of 4 co-authors. Here are the top co-authors by number of shared publications:
- Benjamin D. Williams
University of Illinois, Urbana-Champaign
1 shared publication - Chittanon Buranachai
Center of Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, 1110 W Green St, Loomis Lab, Urbana, IL, 61801, USA
1 shared publication - Daichi Kamiyama
University of Miami
1 shared publication - Robert M. Clegg
Center of Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, U.S.A.
1 shared publication
Latest Publications
- 7 March 2008
- Published by Springer Nature in Journal of Fluorescence
- Vol. 18 (5) , 929-942
Rapid Frequency-Domain FLIM Spinning Disk Confocal Microscope: Lifetime Resolution, Image Improvement and Wavelet Analysis
A spinning disk confocal attachment is added to a full-field real-time frequency-domain fluorescence lifetime-resolved imaging microscope (FLIM). This provides confocal 3-D imaging while retaining all the characteristics of the normal 2-D FLIM. The spinning disk arrangement allows us to retain the speed of the normal 2-D full field FLIM while gaining true 3-D resolution. We also introduce the use of wavelet image transformations into the FLIM analysis. Wavelets prove useful for selecting objects according to their morphology, denoising and background subtraction. The performance of the instrument and the analysis routines are tested with quantitative physical samples and examples are presented with complex biological samples.
- 7 March 2008
- Published by Springer Nature in Journal of Fluorescence
- Vol. 18 (5) , 929-942
Rapid Frequency-Domain FLIM Spinning Disk Confocal Microscope: Lifetime Resolution, Image Improvement and Wavelet Analysis
A spinning disk confocal attachment is added to a full-field real-time frequency-domain fluorescence lifetime-resolved imaging microscope (FLIM). This provides confocal 3-D imaging while retaining all the characteristics of the normal 2-D FLIM. The spinning disk arrangement allows us to retain the speed of the normal 2-D full field FLIM while gaining true 3-D resolution. We also introduce the use of wavelet image transformations into the FLIM analysis. Wavelets prove useful for selecting objects according to their morphology, denoising and background subtraction. The performance of the instrument and the analysis routines are tested with quantitative physical samples and examples are presented with complex biological samples.