Laurent Urban

In plants, environmental stresses trigger reactive oxygen species (ROS) overproduction, causing oxidative damage but also generating systemic signals that prime defense responses in distal organs. How such signals are transmitted from leaves to fruits is a key question for crop stress tolerance and quality. Here, tomato plants were subjected to mercury chloride (5 ppm HgCl2 ) for 24 hours to induce a selective arrest of water flux and to evaluate oxidative parameters and antioxidant defense in leaves, fruit peduncles and fruits. Mercury treatment induced oxidative damage — evidenced by increased H2O2 and MDA accumulation — in leaves and fruit peduncles, while fruits remained unaffected. Nevertheless, antioxidant enzyme activities (SOD, CAT, APX, DHAR and MDHAR) and transcript levels of SlAPXcyto, SlAPXt, SlDHAR1, SlDHAR2 and SlMDHAR were upregulated in fruits, despite the absence of local oxidative stress, suggesting a systemic leaf-to-fruit signal priming antioxidant defenses. To test this hypothesis, detached tomato fruits were treated with four putative stress signals: abscisic acid (ABA), exogenous H2 O2 , nitric oxide (NO, via sodium nitroprusside, SNP) and varying ascorbate (AsA) redox states. Oxidative parameters (H2 O2 and MDA), ascorbate levels, and antioxidant enzyme activities and transcript levels were monitored at 4, 8 and 24 h after treatment. The results indicate that ABA, AsA redox state, H2 O2 and NO each modulate antioxidant enzyme activities and gene expression in tomato fruits. Notably, while ABA, H2 O2 and AsA redox state generally induced antioxidant enzyme activities, NO acted primarily as a direct ROS scavenger, suppressing enzymatic induction, an effect reversed by the NO scavenger cPTIO. Frequent dissociation between activity and transcript levels indicates post-transcriptional regulation. These findings support a model in which ABA, H2 O2 act as interconnected systemic signals co-ordinating antioxidant defense in fruits under leaf stress.

The present study investigated the impact of mercury exposure on plant water status, oxidative stress markers, and antioxidant defense systems in leaves and fruits of tomato (Solanum lycopersicum L. cv. Micro-Tom). Plants were exposed to increasing concentrations of HgCl₂ for 24 and 48 h. Mercury treatment led to a significant reduction in predawn leaf water potential, whereas other water-related parameters in both leaves and fruits remained largely unaffected. Oxidative stress was predominantly observed in leaves, as indicated by elevated hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) levels, while fruit tissues showed no significant accumulation of these oxidative markers. In contrast, fruits exhibited a marked activation of antioxidant defenses, including increased activities of superoxide dismutase (SOD) and catalase (CAT), along with concentrationdependent modulation of ascorbate–glutathione cycle enzymes (APX, MDHAR, DHAR, and GR) and their corresponding transcript levels. Alterations in the ascorbate pool, reflected by changes in reduced ascorbate (AsA) and dehydroascorbate (DHA), further indicated a dynamic regulation of cellular redox status in response to mercury exposure. Collectively, these findings demonstrate that mercury induces tissue-specific oxidative responses and rapidly activates antioxidant mechanisms in fruits, thereby contributing to the maintenance of redox homeostasis and protection against oxidative damage.

Ultraviolet C (UV-C) flash treatment represents a promising method for priming plants. This study compared the effects of 1 s (flash) and 60 s (60 s) UV-C exposures on the transcriptome of Arabidopsis thaliana L. plants. A dose of 200 J m⁻² delivered in one second was observed to effectively stimulate plant defenses without causing any adverse effects on plant health. A total of 3054 and 1865 differentially expressed genes (DEGs) were identified in the flash and 60 s treatments, respectively, in comparison to the control plants. Of these, 1131 were common to both treatments. The flash treatment affected a greater number of transcription factors (415 genes) than the 60 s treatment (254 genes), indicating more pronounced alterations in gene expression. The flash treatment resulted in a significant overexpression of heat shock proteins (HSPs), heat shock factors (HSFs), and their associated genes, which impacted oxidative stress, proteostasis, genome stability, cell survival, and thermotolerance. The majority of mitochondrial genes were found to be upregulated, while photosynthetic genes exhibited a downregulation. These expression patterns coordinate electron transport and crosstalk between the nucleus, chloroplasts, and mitochondria, eliciting an adaptive protective response to UV-C flash. Additionally, the flash treatment resulted in alterations to several genes involved in cell cycle regulation, division, and DNA replication. These included ATP BMMs, BRCA2 s, IQDs, kinesin complex, MCM complex, CYCs, and CDKs, which ultimately led to cell cycle arrest as a temporary preparation for subsequent conditions. The present study demonstrates that a 1 s exposure to UV-C induces distinctive plant responses through coordinated gene expression. The findings suggest that the flash treatment is an innovative method that triggers a unique cellular response, prioritizing repair mechanisms and potentially enhancing plant immunity, resilience, and priming. It can be used as a plant resistance inducer and stimulator.

High salinity results in a decrease in plant photosynthesis and crop productivity. The aim of the present study was to evaluate the effect of UV-C priming treatments of lettuce seeds on photosynthesis of plants grown at high salinity. Non-primed and primed seeds were grown in an hydroponic system, with a standard nutrient solution, either supplemented with 100 mM NaCl (high salinity), or not (control). Considering that leaf and root K⁺ concentrations remained constant and that chlorophyll fluorescence parameters and root growth were not affected negatively in the high salinity treatment, we conclude that the latter was at the origin of a moderate stress only. A substantial decrease in leaf net photosynthetic assimilation (A_net) was however observed as a consequence of stomatal and non-stomatal limitations in the high salinity treatment. This decrease in A_net translated into a decrease in growth parameters; it may be attributed partially to the high salinity-associated increase in leaf concentration in abscisic acid and decrease in stomatal conductance. Priming by UV-C light resulted in an increase in total photosynthetic electron transport rate and A_net in the leaves of plants grown at high salinity. The increase of the latter translated into a moderate increase in growth parameters. It is hypothesized that the positive effect of UV-C priming on A_net and growth of the aerial part of lettuce plants grown at high salinity, is mainly due to its stimulating effect on leaf concentration in salicylic acid. Even though leaf cytokinins’ concentration was higher in plants from primed seeds, maintenance of the cytokinins-to-abscisic acid ratio also supports the idea that UV-C priming resulted in protection of plants exposed to high salinity.

Systemic acquired resistance is a powerful mechanism, based on the salicylic acid (SA) signaling pathway, which allows plants to resist to a wide range of pathogens. High SA, moreover, plays a key role in plant tolerance to abiotic stress. It seems, therefore, desirable to supply analogs of SA or stimulate the production of endogenous SA. Unfortunately, the chemical substances or physical means used for this effect often display a variable efficacy. After providing a review of them, we defend three major ideas: (i) plant resistance inducers (PRIs) must be combined for higher efficacy, notably for exploiting synergic effects between the SA and other signaling pathways, (ii) disease pressure can be reduced by exploiting the fungicidal properties displayed by some PRIs, (iii) biostimulants and crop management techniques should be used to ensure that plants have the resources they need to synthesize the compounds and structures required for efficient and lasting resistance. Some PRIs could also be used for their biostimulant effects in stress conditions. It could be concluded that holistic approaches which jointly address the issues of defense and tolerance stimulation, disease pressure and resource availability in plants are the ones that will allow for substantial reduction in fungicide use without sacrificing crop performance.

The photopolymerization of caffeic acid (3,4-Dihydroxy-trans-cinnamate) was triggered using pulses of UV-C light at 278 nm during which the fluorescence of caffeic acid was measured and can be considered as an indicator for this pho-topolymerization process. We present a technological approach and evaluate the fluorescence signal of caffeic acid excited by pulses of UV-C radiation using a silicon photomultiplier SiPM in Geiger mode. Furthermore, liquid chromatography - mass spectrometry (LC-MS) analysis showed that a dimer of caffeic acid was successfully photodimerized.

Using detached leaves, UV-C light in the form of 1-s flashes has recently been shown to stimulate defenses of several plants against different pathogens better than 1-min exposures under greenhouse conditions. In the present work, the pathological tests were conducted using undetached leaves under greenhouse and vineyard conditions. In a first trial, two flashes of UV-C light were applied to plants of Vitis vinifera L. ‘Chardonnay’ grown under greenhouse conditions, at an interval of 10 days. Plants were inoculated with Erysiphe necator 2 days after the last light treatment. After 18 days of inoculation, the symptom severity on leaves was reduced by 60% when compared with the untreated control. In a second trial, flashes of UV-C light were applied to grapevine Chardonnay plants under field conditions in the southeast of France every 10 days from 18 April until 10 July 2019. The symptom severity resulting from natural contaminations by E. necator was reduced by 42% in leaves on 4 July 2019 and by 65% in clusters on 25 July 2019. In a third trial, we observed that UV-C light did not have any effect on net photosynthesis, maximal net photosynthesis, dark respiration, maximal quantum efficiency of photosystem II, the performance index of Strasser, and, generally, any parameter derived from induction curves of maximal chlorophyll fluorescence. It was concluded that flashes of UV-C light have true potential for stimulating plant defenses against E. necator under vineyard conditions and, therefore, help in reducing fungicide use.

Ultraviolet-C (UV-C) radiation is efficient in reducing the development of diseases in many species, including strawberry (Fragaria × ananassa). Several studies suggest that UV-C radiation is effective not only because of its disinfecting effect but also because it may stimulate plant defenses. In this study, the effect of preharvest UV-C radiation applied during strawberry cultivation on plant growth, fruit quality, and susceptibility to major fungal diseases such as gray mold, powdery mildew, and soft rot was evaluated. UV-C treatments had an impact on flowering initiation and fruit development. Flowering occurred earlier for UV-C-treated plants than for nontreated plants. At harvest, a larger amount of fruit was produced by treated plants despite their slight decrease in leaf area. UV-C treatment did not improve strawberry shelf life but did not alter the physical integrity of strawberry fruit. Natural infection of leaves to powdery mildew and of fruit to Rhizopus spp. strongly decreased in response to UV-C treatment.

Leaves of lettuce, pepper, tomato and grapevine plants grown in greenhouse conditions were exposed to UV-C light for either 60 s or 1 s, using a specific LEDs-based device, and wavelengths and energy were the same among different light treatments. Doses of UV-C light that both effectively stimulated plant defences and were innocuous were determined beforehand. Tomato plants and lettuce plants were inoculated with Botrytis cinerea, pepper plants with Phytophthora capsici, and grapevine with Plasmopara viticola. In some experiments we investigated the effect of a repetition of treatments over periods of several days. All plants were inoculated 48 h after exposure to the last UV-C treatment. Lesions on surfaces were measured up to 12 days after inoculation, depending on the experiment and the pathogen. The results confirmed that UV-C light stimulates plant resistance; they show that irradiation for one second is more effective than irradiation for 60 s, and that repetition of treatments is more effective than single light treatments. Moreover a systemic effect was observed in unexposed leaves that were close to exposed leaves. The mechanisms of perception and of the signalling and metabolic pathways triggered by flashes of UV-C light vs. 60 s irradiation exposures are briefly discussed, as well as the prospects for field use of UV-C flashes in viticulture and horticulture.

One (1P), two (2P), three (3P) or four (4P) pulses of light supplied by a xenon lamp, were applied to young lettuce plants grown in pots. The lamp used in the trial was similar to those used for fruit surface sterilization. Total flavonols were measured in leaves using the Dualex method. In a first trial conducted in greenhouse conditions, 6 days after the pulsed light (PL) treatment, flavonols were increased by 312% and 525% in the 3P and 4P treatments, respectively, in comparison to the those in the untreated control. Changes in the chlorophyll fluorescence parameters suggest that the PL treatment may induce limited and transient damage to the photosynthetic machinery and that the damage increases with the increasing number of pulses. The performance parameters were not significantly affected by PL and recovered fully by 6 days after the treatments. The 1P and the 2P treatments 6 days after the treatment showed a 28.6% and a 32.5% increase, respectively, in net photosynthetic assimilation, when compared to that of the control. However, 8 days after the treatment, there was no longer a difference between the treatments and the control in net photosynthetic assimilation. Eight days after the light treatment, the 3P treatment showed a 38.4% increase in maximal net photosynthetic assimilation over that of the control, which is an indication of positive long-term adaptation of photosynthetic capacity. As a whole, our observations suggest that PL could be used on field or greenhouse crops to increase their phytochemical content. No long-lasting or strong negative effects on photosynthesis were associated with PL within the range of doses we tested; some observations even suggest that certain treatments could result in an additional positive effect. This conclusion is supported by a second trial conducted in phytotrons. More studies are required to better understand the roles of the different wavelengths supplied by PL and their interactions.