Daniel J. Franklin

World Health Organization (WHO) guidance on the management of cyanobacteria in recreational waters provides a comprehensive framework for reducing human health risks. To provide a global overview of how cyanobacterial risks in recreational waters are managed and to better understand capacity gaps and best practice, we surveyed expert opinion across the world. A total of 161 survey responses were collected from 49 countries. Results indicate widespread variation in the extent of recreational water monitoring. Routine monitoring often varies by region in larger, more populous countries, while seasonal monitoring, typically conducted at variable intervals, was the most used approach. Approximately half of the countries reported either a lack of routine monitoring or a combination of different monitoring patterns. Notably, whereas nearly all countries reported methods for planktonic sampling, only one-third of countries reported methods for benthic sampling. Globally, we conclude that the assessment and management of benthic cyanobacteria risks lag behind that of planktonic cyanobacteria risks. Cell counts and pigments were the most common assessment techniques for planktonic cyanobacteria. Microcystis and Oscillatoria were the most frequently reported problematic genus of planktonic and benthic cyanobacteria, respectively, and microcystins were the most frequently reported cyanotoxins of concern. A wide variety of management responses and risk communication methods were reported with the primary action trigger being the presence of cyanobacterial scums. Experts across the world were mostly unsatisfied with recreational water risk management regimes, with over 80% of respondents desiring change in how cyanobacterial risks are managed. We conclude that a renewed international effort is needed to enhance collaboration and knowledge exchange and sustain investment to ensure safer recreational waters at a global scale.

Cyanobacteria are ancient and versatile members of almost all aquatic food webs. In freshwater ecosystems some cyanobacteria form “bloom” populations containing potent toxins and such blooms are therefore a key focus of study. Bloom populations can be ephemeral, with rapid population declines possible, though the factors causing such declines are generally poorly understood. Cell death could be a significant factor linked to population decline. Broadly, three forms of cell death are currently recognized – accidental, regulated and programmed – and efforts are underway to identify these and standardize the use of cell death terminology, guided by work on better-studied cells. For cyanobacteria, the study of such differing forms of cell death has received little attention, and classifying cell death across the group, and within complex natural populations, is therefore hard and experimentally difficult. The population dynamics of photosynthetic microbes have, in the past, been principally explained through reference to abiotic (“bottom-up”) factors. However, it has become clearer that in general, only a partial linkage exists between abiotic conditions and cyanobacteria population fluctuations in many situations. Instead, a range of biotic interactions both within and between cyanobacteria, and their competitors, pathogens and consumers, can be seen as the major drivers of the observed population fluctuations. Whilst some evolutionary processes may theoretically account for the existence of an intrinsic form of cell death in cyanobacteria, a range of biotic interactions are also likely to frequently cause the ecological incidence of cell death. New theoretical models and single-cell techniques are being developed to illuminate this area. The importance of such work is underlined by both (a) predictions of increasing cyanobacteria dominance due to anthropogenic factors and (b) the realization that influential ecosystem modeling work includes mortality terms with scant foundation, even though such terms can have a very large impact on model predictions. These ideas are explored and a prioritization of research needs is proposed.

Freshwater cyanobacteria blooms represent a risk to ecological and human health through induction of anoxia and release of potent toxins; both conditions require water management to mitigate risks. Many cyanobacteria taxa may produce microcystins, a group of toxic cyclic heptapeptides. Understanding the relationships between the abiotic drivers of microcystins and their occurrence would assist in the implementation of targeted, cost-effective solutions to maintain safe drinking and recreational waters. Cyanobacteria and microcystins were measured by flow cytometry and liquid chromatography coupled to tandem mass spectrometry in two interconnected reservoirs varying in age and management regimes, in southern Britain over a 12-month period. Microcystins were detected in both reservoirs, with significantly higher concentrations in the southern lake (maximum concentration >7 µg L⁻¹). Elevated microcystin concentrations were not positively correlated with numbers of cyanobacterial cells, but multiple linear regression analysis suggested temperature and dissolved oxygen explained a significant amount of the variability in microcystin across both reservoirs. The presence of a managed fishery in one lake was associated with decreased microcystin levels, suggestive of top down control on cyanobacterial populations. This study supports the need to develop inclusive, multifactor holistic water management strategies to control cyanobacterial risks in freshwater bodies.

Cyanobacteria contribute a significant fraction of global primary production and are therefore of great ecological significance. An individual cyanobacteria cell has four potential fates: to divide, perhaps after a dormant period, to be eaten, to undergo viral lysis or to undergo cell death. In some studies, cyanobacteria cell death has been classified as programmed cell death, borrowing a concept more widely known in metazoan cells, and there are various biochemical parallels to support such a categorization. However, at the same time, there is a growing awareness of asymmetric division as a fundamental process in bacterial division which can result in non-equal daughter cells with differing fitness. Thanks to recent theoretical and experimental advances, it is now possible to explore cyanobacteria cell death in the light of asymmetric division and to test hypotheses on the ultimate causes of cyanobacterial cell death. Assessing the degree of protein damage within individual cells during population growth is a sensible initial research target as is the application of techniques which allow the tracking of cell lineages. The existence of asymmetric division in cyanobacteria is likely given its suggested ubiquity across the bacterial domain of life. It will be technically difficult to test the interaction of asymmetric division with environmental variability, and how that leads to individual cell death via differing susceptibilities to environmental stress. However, testing such ideas could confirm asymmetric division as the ultimate cause of cell death in cyanobacteria and thereby allow a better understanding of the patterns of cell death in natural populations.