Gahdab Chakali

Wildfire is a dominant disturbance in Mediterranean cork oak (Quercus suber) ecosystems, yet its medium-term consequences for insect assemblages remain poorly documented in North Africa. We compared burnt and unburnt stands in four suberaies of north-eastern Algeria (Djebel El Goufi/Lemdjalba, El Maktoua, Oued Agouf and Ain Fegoune) during two consecutive post-fire years (2015 and 2016). Across 16 station-year units, 402 insect species were recorded. Unburnt stands harboured 364 species against 111 in burnt stands, and 291 species were exclusive to unburnt stands. Sorensen similarity between burnt and unburnt stands was consistently low (mean 18.4% in 2015 and 10.8% in 2016), whereas unburnt stands remained comparatively stable between years (mean 61.9%) and burnt stands did not (22.5%). Principal component analysis of Hellinger-transformed abundances separated burnt from unburnt units along PC1, and PERMANOVA confirmed a significant effect of fire history (R2 = 0.25, p = 0.0001; ANOSIM R = 0.72, p < 0.001) with no significant year or interaction effect. UpSet and chord diagrams showed that species overlap was concentrated within, rather than between, fire-history classes, with only one to two species shared by all eight stations. Two years after fire, assemblages had not begun to converge towards the unburnt reference, indicating slow entomofaunal resilience and supporting the retention of unburnt refuges in post-fire management.

The sustainable protection of cedar stands in Chréa National Park can only be accomplished through the stability of the ecosystem. Outbreaks of Thaumetopoea pityocampa are a major threat and are largely attributed to the high population fecundity, changes in the diversity of natural enemies and global interactions within the ecosystem. Egg parasitoids (Hymenoptera) are essential in the biological control of T. pityocampa. To assess the impact of the parasitoids on the populations of the pine processionary moth, egg masses from cedar plantations were collected, reared in a laboratory and checked regularly for the emergence of the egg parasitoids: Trichogramma embryophagum, Baryscapus servadeii and Ooencyrtus pityocampa. Observations showed an inter-annual variation in the abundance of the three parasitoids as a result of the variation in the population density of the processionary moth, and on the underlying effect of temperature. Parasitoids had variable parasitism rates, with yearly averages ranging from 3.86% to 51.14%, dependent on the spatiotemporal distribution of the host populations. The aggregate effect of multiple parasitoid species could optimize control of T. pityocampa in cedar stands.

Cotula cinerea, belonging to the tribe Anthemideae, is a plant widespread in the Southern hemisphere. It is frequently used in folk medicine in North African countries for several of its medical properties, shown by its extracts and essential oils. The dichloromethane extract obtained from its aerial parts demonstrated antibiotic activity against Enterococcus faecalis and was fractionated by bioguided purification procedures affording five main sesquiterpene lactones. They were identified by spectroscopic methods (NMR and ESIMS data) as guaiantrienolides, i.e., 6-acetoxy-1β-,6-acetoxy-1α-, and 6-acetoxy-10-β-hydroxyguaiantrienolide (1–3), and germacrenolides, i.e., haagenolide and 1,10-epoxyhaagenolide (4 and 5). The absolute configuration was assigned by applying the advanced Mosher’s method to haagenolide and by X-ray diffraction analysis to 1,10-epoxyhaagenolide. The specific antibiotic and antibiofilm activities were tested toward the clinical isolates of Enterococcus faecalis. The results showed that compounds 3–5 have antibacterial activity against all the strains of E. faecalis, while compound 2 exhibited activity only toward some strains. Compound 1 did not show this activity but had only antibiofilm properties. Thus, these metabolites have potential as new antibiotics and antibiofilm against drug resistant opportunistic pathogens.

Morphological and molecular studies were conducted to characterize the specific identity of 36 isolates of potato cyst nematodes (PCNs) recovered from soil samples collected in several potato producing areas of Algeria. Morphometric data revealed that 44% of isolates contained Globodera pallida alone, 28% contained Globodera rostochiensis alone and 28% mixtures of the two species. Morphometric values of cysts and second-stage juveniles were generally distributed with slight differences in the expected ranges for both Globodera species. Inter- and intraspecific morphometric variability in nematode isolates was noted. Molecular analysis using conventional multiplex PCR with species-specific primers and TaqMan real-time PCR confirmed the morphological identification. In addition, the distribution of both potato cyst nematode species throughout various parts of the country was investigated. In the central areas, the isolates of G. pallida alone dominate, whereas isolates of G. rostochiensis alone are more frequent in the southern areas. In the eastern regions, mixed isolates are more representative. Most isolates examined in the western areas are mixtures of the two species or G. rostochiensis alone. Comparatively, G. pallida remains the most widely distributed species in its geographic range. This study confirms the presence of two PCN species, G. pallida and G. rostochiensis, in Algeria and provides additional information on their biogeographic distribution.