Aleksandra Wawro

The cultivation of industrial hemp (Cannabis sativa L.) has increased dynamically in recent years, driven by its versatile applications across various sectors, including agriculture, energy, materi...

Kwasy tłuszczowe są niezbędne do prawidłowego funkcjonowania organizmu człowieka – budują błony komórkowe, wspierają pracę mózgu i układu nerwowego. Organizm nie potrafi samodzielnie syntetyzować niezbędnych nienasyconych kwasów tłuszczowych (NNKT), dlatego ich źródłem musi być dieta, bogata głównie w kwasy omega-3 (ALA) i omega-6 (LA). Naturalnym i skutecznym sposobem suplementacji NNKT są oleje roślinne zawierające także witaminę E i antyoksydanty, które dodatkowo wspomagają ochronę organizmu przed stresem oksydacyjnym. Profil kwasów tłuszczowych w nowych genotypach oleistych gatunków roślin włóknistych i zielarskich jest jednym z najważniejszych kierunków prac hodowlanych, gdyż pozwala łączyć wysoką wartość odżywczą z funkcjonalnością technologiczną. Wysoka zawartość NNKT w odmianach lnu, konopi siewnych, ostropestu czy kolendry umożliwia zwiększenie udziału bioaktywnych związków, co ma ogromne znaczenie dla dietoprofilaktyki i rozwoju żywności prozdrowotnej. Równocześnie badania nad zmiennością genetyczną i optymalizacją uprawy tych roślin pozwalają na opracowanie nowych źródeł naturalnych olejów roślinnych o pożądanym składzie kwasów tłuszczowych, wspierających zdrowie człowieka. Szczególnie istotne jest określenie relacji pomiędzy czynnikami środowiskowymi a profilem lipidowym roślin, co pozwala na dobór optymalnych warunków agrotechnicznych.

The aim of this study was to develop a natural nonwoven layer made of cottonized bleached flax and cotton fibers which is suitable to replace one of the three polypropylene layers of face mask type II in order to reduce non-biodegradable waste production and limit the negative impact of used masks on the environment. The work focused on the design of a nonwoven structure based on properly blending cotton and flax fibers as well as ensuring the cover factor, which can support the mask’s barrier properties against air dust particles and does not make breathing difficult. Additionally, a biodegradable film was developed to connect the nonwoven layer with the other polypropylene filtering layers. The effectiveness of the biodeterioration of the flax/cotton nonwoven was evaluated based on a test of the susceptibility of materials to the action of soil microorganisms. The flax/cotton nonwoven layer was tested in terms of mechanical, physical, and biophysical properties, and an analysis of the covering of the nonwoven surface with fibers was conducted as well. The results confirmed that the structure of flax/cotton nonwovens is suitable to replace the nondegradable polypropylene layer of the face mask type II to improve its environmental performance.

Soil salinity is one of the particularly important environmental factors, which limits the growth and development of plants, reduces the amount of crops and causes serious economic damage. The study found that Festuca arundinacea can be successfully used for the management and bioremediation of saline habitats, and it is characterized by a very well-developed root system and an average high biomass production of approx. 14.8 Mg∙ha⁻¹, which can be used for industrial applications. Tall fescue biomass from soils with different levels of salinity was used to obtain bioethanol using 1.5% NaOH pretreatment and for the process of simultaneous enzymatic hydrolysis and ethanol fermentation (SSF). It was found that the content of Na⁺ ions in the tall fescue biomass had no significant effect on the amount of ethanol obtained (the average 19.32 g∙100 g⁻¹ of raw material). F. arundinacea biomass was also used as a natural filler to obtain green composites using a biodegradable polylactide (PLA) matrix. It has been shown that tall fescue biomass with the lowest sodium content in biomass (approx. 2.5 mg∙g⁻¹) from an area with high saline (above 6 g NaCl∙L⁻¹) has the smallest effect on reducing the tensile and flexural strength of composites. Moreover, the highest value of bioethanol concentration (21.2 g∙100 g⁻¹ of raw material) for this biomass sample was obtained.

Flax is one of the few plants that are entirely a source of raw materials for further production. Promising directions for the use of flax biomass may be the production of bioenergy in the form of 2G biofuels and the production of “green” composites. The aim of the study is to compare the biomass of fiber flax, linseed and dual-purpose varieties of cultivated flax (Linum usitatissimum L.) susceptibility to the biorefining processes. In the first stage of the research, based on the results of yield structure features and biometric measurements of plants, the most optimal flax line was selected for the fiber flax, linseed and dual purpose. Next, the forms of flax were pretreated with sodium hydroxide (NaOH), the chemical composition was determined and SEM images were taken. The obtaining of bioethanol process SFF (simultaneous saccharification and fermentation) was carried out. In addition, biodegradable polymers were modified with flax biomass, shapes were prepared, and the rheological and mechanical properties, as well as microbiological activity of biocomposites, were determined. The highest concentration of ethanol (8.72 g·L⁻¹) and the greatest susceptibility to mold fungi of the biocomposites were obtained for the fiber flax variety PET 16/20.

Currently, fossil fuels are used to produce fuels and electricity, which are finite sources and have a negative impact on the natural environment. An excellent alternative to these fuels is biofuels, such as bioethanol from waste forest biomass. Pine needles are one of the most important available forest biomass materials with s significant impact on local understory vegetation. Forest waste biomass, which is a rich source of lignocellulose, can be used in various ways, such as for the eco-economical production of bioethanol. The aim of this study was to analyze the possibilities of bioethanol production from pine needle biomass obtained from forest land following different soil preparations and logging residue management. The pine needle dry matter yield, chemical components of pine needle biomass (cellulose, hemicellulose, lignin), and the amount of ethanol yield per hectare were evaluated. The highest average yield pine needle equal to 6.17 Mg∙ha⁻¹ was observed. Bioethanol yield per hectare from this biomass was the highest for plowing with the LPZ-75 plow and was 1.08 m³∙ha⁻¹. The discussed results were confirmed by detailed statistical analysis. To sum up, the researched pine needle biomass turned out to be an interesting raw material with the potential for bioethanol production.

Due to the threat posed by the spread of invasive plant species, there is an urgent need to develop effective methods of eradicating and managing their biomass. The aim of the study was to examine selected invasive plants in terms of their use for energy purposes and to find out whether they can be a raw material for the production of second-generation biofuels. First, their chemical compositions were determined. The higher heating value (HHV) and lower heating value (LHV) were also calculated. High values of the higher heating value, ranging from 18.490 MJ∙kg⁻¹ to 19.900 MJ∙kg⁻¹, indicate the possibility of using the biomass of invasive plants for energy purposes (combustion). All investigated invasive plant species were also subjected to the process of obtaining ethanol. This included an alkaline pretreatment with 1% sodium hydroxide, followed by a simultaneous saccharification and fermentation (SSF) process. The highest ethanol yield per ha of plants was obtained at 2.6 m³∙ha⁻¹ for the Reynoutria × bohemica biomass. The remaining species showed an ethanol yield below 2 m³∙ha⁻¹. The conducted research allows for the conclusion that the studied invasive plants can be a promising raw material for the production of bioethanol.

The problem of global warming is still a major issue, alongside shrinking oil reserves. A great alternative to fossil fuels is offered by biofuels, such as bioethanol from lignocellulosic plants. The sorghum biomass can be effectively used in many industrial directions. It is possible to use every part of this plant; the grain can be used for food production and straw can be used for energy purposes, i.e., for bioethanol. The aim of this study was to analyze the possibilities of bioethanol production from five varieties of sorghum biomass, which is a waste product of seed harvesting. The yields of sorghum cultivars in a three-year vegetation period; the amount of cellulose, hemicellulose, and lignin in the biomass of sorghum; and the amount of ethanol obtained per hectare were evaluated. It was observed that the highest average yield for all cultivars, except GK Emese, was found in the second year of the study. The bioethanol yield per hectare from this biomass was the highest for Sweet Caroline and was 9.48 m³∙ha⁻¹. In addition, significant differences were found in the content of lignin and hemicellulose for the varieties tested in all years of the study and for the content of cellulose in the first and third years. The discussed results were confirmed by detailed statistical analyses, including combined matrices of Pearson correlation coefficients (cr_p) varieties and cluster analysis. In summary, the usefulness of the biomass of the studied sorghum varieties for the production of bioethanol was demonstrated.

Nowadays, more and more attention is paid to the development and the intensification of the use of renewable energy sources. Hemp might be an alternative plant for bioenergy production. In this paper, four varieties of Polish industrial hemp (Białobrzeskie, Tygra, Henola, and Rajan) were investigated in order to determine which of them are the most advantageous raw materials for the effective production of bioethanol. At the beginning, physical and chemical pretreatment of hemp biomass was carried out. It was found that the most effective is the alkaline treatment with 2% NaOH, and the biomasses of the two varieties were selected for next stages of research: Tygra and Rajan. Hemp biomass before and after pretreatment was analyzed by FTIR and SEM, which confirmed the effectiveness of the pretreatment. Next, an enzymatic hydrolysis process was carried out on the previously selected parameters using the response surface methodology. Subsequently, the two approaches were analyzed: separated hydrolysis and fermentation (SHF) and a simultaneous saccharification and fermentation (SSF) process. For Tygra biomass in the SHF process, the ethanol concentration was 10.5 g∙L⁻¹ (3.04 m³·ha⁻¹), and for Rajan biomass at the SSF process, the ethanol concentration was 7.5 g∙L⁻¹ (2.23 m³·ha⁻¹). In conclusion, the biomass of Polish varieties of hemp, i.e., Tygra and Rajan, was found to be an interesting and promising raw material for bioethanol production.

The salinity of European soil is increasing every year, causing severe economic damage (estimated 1–3 million hectares in the enlarged EU). This study uses the biomass of halophytes—tall fescue (grass) and hemp of the Białobrzeskie variety from saline soils—for bioenergy, second generation biofuels and designing new materials—fillers for polymer composites. In the bioethanol obtaining process, in the first stage, the grass and hemp biomass were pretreated with 1.5% NaOH. Before and after the treatment, the chemical composition was determined and the FTIR spectra and SEM pictures were taken. Then, the process of simultaneous saccharification and fermentation (SSF) was carried out. The concentration of ethanol for both the grass and hemp biomass was approx. 7 g·L⁻¹ (14 g·100 g⁻¹ of raw material). In addition, trials of obtaining green composites with halophyte biomass using polymers (PP) and biopolymers (PLA) as a matrix were performed. The mechanical properties of the composites (tensile and flexural tests) were determined. It was found that the addition of a compatibilizer improved the adhesion at the interface of PP composites with a hemp filler. In conclusion, the grass and hemp biomass were found to be an interesting and promising source to be used for bioethanol and biocomposites production. The use of annually renewable plant biomass from saline soils for biorefinering processes opens up opportunities for the development of a new value chains and new approaches to sustainable agriculture.