Humaira Gul

Saline environments for plants have a negative impact on their biochemical, molecular, and physiological features, resulting in a loss in plant growth and yield in most parts of the world. Hormonal imbalance, ionic toxicity, ROS production, impaired nutrient mobilization, and osmotic stress all contribute to plant growth loss. The symbiotic connection of several endophytes with their host plants has improved plant tolerance to various abiotic challenges in recent years. So, the current study aimed to see how Aspergillus japonicus and salinity affected growth parameters, plant biochemistry, antioxidant enzyme status, endogenous IAA, and various anion and cation concentrations in okra. According to the findings, applying salt lowered numerous growth properties, including Chlorophyll a and b, total chlorophyll, and endogenous IAA. Enhanced values of a/b (chlorophyll), total carotenoids, total proteins, total carbs, total lipids, total proline, total phenol, total flavonoids, total tannin, lipid peroxidation, catalase, and ascorbate peroxidase were seen at similar salt levels. In okra (Sanaf green), salt application resulted in salt accumulation and higher levels of Cl, Na, Ca/K, and Na/K ratio, while low contents of K, P, Mg, Ca, O, N, and C were found. The results of this study show that using Aspergillus japonicus promotes plant resistance to saltwater environments, as evidenced by the fact that using the fungal strain increased the values of many growth parameters, metabolites, anti-oxidant enzyme activity, and endogenous IAA. In the same way, using Aspergillus japonicus lowered the amount of lipid peroxidation in the okra plant. The accumulation of salts in okra after application of Aspergillus japonicus resulted in an increase in Ca, Mg, N, C, K, Cl, O, and P values, as well as a decrease in the Ca/K and Na/K ratios, Cl, and Na values. According to the findings, salt stress causes stunted development and altered biochemistry in okra, while the presence of Aspergillus japonicus aids in plant salt tolerance. As a result, this strain is an excellent candidate for testing as a biostimulant/biofertilizer to improve the growth of essential crops in Pakistan's salt-affected areas.

Abiotic stressors are global limiting constraints for plant growth and development. The most severe abiotic factor for plant growth suppression is salt. Among many field crops, maize is more vulnerable to salt, which inhibits the growth and development of plants and results in low productivity or even crop loss under extreme salinity. Consequently, comprehending the effects of salt stress on maize crop improvement, while retaining high productivity and applying mitigation strategies, is essential for achieving the long-term objective of sustainable food security. This study aimed to exploit the endophytic fungal microbe; Aspergillus welwitschiae BK isolate for the growth promotion of maize under severe salinity stress. Current findings showed that salt stress (200 mM) negatively affected chlorophyll a and b, total chlorophyll, and endogenous IAA, with enhanced values of chlorophyll a/b ratio, carotenoids, total protein, total sugars, total lipids, secondary metabolites (phenol, flavonoids, tannins), antioxidant enzyme activity (catalase, ascorbate peroxidase), proline content, and lipid peroxidation in maize plants. However, BK inoculation reversed the negative impact of salt stress by rebalancing the chlorophyll a/b ratio, carotenoids, total protein, total sugars, total lipids, secondary metabolites (phenol, flavonoids, tannins), antioxidant enzyme activity (catalase, ascorbate peroxidase), and proline content to optimal levels suitable for growth promotion and ameliorating salt stress in maize plants. Furthermore, maize plants inoculated with BK under salt stress had lower Na⁺, Cl⁻ concentrations, lower Na^+/K⁺ and Na⁺/Ca²⁺ ratios, and higher N, P, Ca²⁺, K⁺, and Mg²⁺ content than non-inoculated plants. The BK isolate improved the salt tolerance by modulating physiochemical attributes, and the root-to-shoot translocation of ions and mineral elements, thereby rebalancing the Na⁺/K⁺, Na⁺/Ca²⁺ ratio of maize plants under salt stress.

| Crop productivity mainly depends on soil type, soil composition and texture particularly, the ratio of sand and clay. The current research was carried out to investigate the effect of different ratios of sand and clay composition on growth and biochemical aspects of Barley ( Hordeum vulgare L.) and variations in soil physical and chemical properties were tested. During the experiment, plants were grown in eleven sets of soil combinations texture wise with varying ratio of sand and clay along their chemical properties. Different growth parameters based on physiological functioning and biochemical aspects were observed for various physical and chemical properties of soil provided to plants for growth optimization.. Barley flourished only well in sandy soil. The number of leaves, branches, fruits, plant height, proteins, and chlorophylls were significantly (P<0.05) increased in sandy soil proportion whereas in soil analysis moisture, hygroscopic water, pH, cation exchange capacity (CEC), chlorides, organic matter were significantly (P<0.05) increased for the soily soil in same proportion that is of sandy soil. This reveals that Barley should be grown in sandy soil for its better growth and productivity. The least growth parameters were noted on soil with more clay retaining most of moisture and inhibiting growth due to soil pH.

Global climate change has imposed harsh environmental conditions such as drought. Naturally, the most compatible fungal consortia operate synergistically to enhance plant growth and ecophysiological responses against abiotic strains. Yet, little is known about the interactions between phytohormone-producing endophytic fungal symbionts and plant growth under drought stress. The existing research was rationalized to recognize the role of newly isolated drought-resistant, antioxidant-rich endophytic fungal consortia hosting a xerophytic plant, Carthamus oxycantha L., inoculated to Moringa oleifera L. grown under drought stress of 8% PEG (polyethylene glycol-8000). Under drought stress, the combined inoculation of endophytic strain Microdochium majus (WA), Meyerozyma guilliermondi (TG), and Aspergillus aculeatus (TL3) exhibited a significant improvement in growth attributes such as shoot fresh weight (1.71-fold), shoot length (0.86-fold), root length (0.65-fold), dry weight (2.18-fold), total chlorophyll (0.46-fold), and carotenoids (0.87-fold) in comparison to control (8% PEG). Primary and secondary metabolites were also increased in M. oleifera inoculated with endophytic consortia, under drought stress, such as proteins (1.3-fold), sugars (0.58-fold), lipids (0.41-fold), phenols (0.36-fold), flavonoids (0.52-fold), proline (0.6-fold), indole acetic acid (IAA) (4.5-fold), gibberellic acid (GA) (0.7-fold), salicylic acid (SA) (0.8-fold), ascorbic acid (ASA) (1.85-fold), while abscisic acid (ABA) level was decreased (−0.61-fold) in comparison to the control (8% PEG). Under drought stress, combined inoculation (WA, TG, TL3) also promoted the antioxidant activities of enzymes such as ascorbate peroxidase (APX) (3.5-fold), catalase (CAT) activity (1.7-fold), and increased the total antioxidant capacity (TAC) (0.78-fold) with reduced reactive oxygen species (ROS) such as H₂O₂ production (−0.4-fold), compared to control (8% PEG), and stomatal aperture was larger (3.5-fold) with a lesser decrease (−0.02-fold) in water potential. Moreover, combined inoculation (WA, TG, TL3) up regulated the expression of MolHSF3, MolHSF19, and MolAPX genes in M. oleifera under drought stress, compared to the control (8% PEG), is suggestive of an important regulatory role for drought stress tolerance governed by fungal endophytes. The current research supports the exploitation of the compatible endophytic fungi for establishing the tripartite mutualistic symbiosis in M. oleifera to alleviate the adverse effects of drought stress through strong antioxidant activities.

Downy mildew (DM), caused by P. cubensis, is harmful to cucurbits including luffa, with increased shortcomings associated with its control through cultural practices, chemical fungicides, and resistant cultivars; there is a prompt need for an effective, eco-friendly, economical, and safe biocontrol approach. Current research is therefore dealt with the biocontrol of luffa DM1 through the endophytic fungi (EF) consortium. Results revealed that T. harzianum (ThM9) and T. virens (TvA1) showed pathogen-dependent inducible metabolic production of squalene and gliotoxins by higher gene expression induction of SQS1/ERG9 (squalene synthase) and GliP (non-ribosomal peptide synthetase). Gene expression of lytic enzymes of EF was also induced with subsequently higher enzyme activities upon confrontation with P. cubensis. EF-inoculated luffa seeds showed efficient germination with enhanced growth potential and vigor of seedlings. EF-inoculated plants showed an increased level of growth-promoting hormone GA with higher gene expression of GA2OX8. EF-pre-inoculated seedlings were resistant to DM and showed an increased GSH content and antioxidant enzyme activities (SOD, CAT, POD). The level of MDA, H₂O_2, REL, and disease severity was reduced by EF. ACC, JA, ABA, and SA were overproduced along with higher gene expression of LOX, ERF, NCED2, and PAL. Expression of defense-marker genes (PPO, CAT2, SOD, APX, PER5, LOX, NBS-LRR, PSY, CAS, Ubi, MLP43) was also modulated in EF-inoculated infected plants. Current research supported the use of EF inoculation to effectively escalate the systemic immunity against DM corresponding to the significant promotion of induced systemic resistance (ISR) and systemic acquired resistance (SAR) responses through initiating the defense mechanism by SA, ABA, ET, and JA biosynthesis and signaling pathways in luffa.

Salt stress is one of the abiotic stresses which negatively affects the growth and yield of different plants world-wide. The purpose of the present investigation was to analyze the physiological and biochemical parameters of maize plants exposed to salt stress with or without fungal association. The results indicated that salinity negatively affected the growth attributes, biochemical parameters and ionic status in maize. In contrast, WSQ association has improved the growth parameters, antioxidant enzyme activity, IAA and metabolites, N, P, Ca, K and Mg concentrations in maize. Moreover, WSQ association reduced lipid-peroxidation, Na, Cl, Na/K and Ca/K ratio in maize during salinity stress. The results of the present study concluded that the endophyticfungal strain WSQ can serve as a good strategic-tool to improve maize growth under salt-stress.

In the current study, we aimed to elucidate the plant growth-promoting characteristics of Pseudomonas psychrotolerans CS51 under heavy metal stress conditions (Zn, Cu, and Cd) and determine the genetic makeup of the CS51 genome using the single-molecule real-time (SMRT) sequencing technology of Pacific Biosciences. The results revealed that inoculation with CS51 induced endogenous indole-3-acetic acid (IAA) and gibberellins (GAs), which significantly enhanced cucumber growth (root shoot length) and increased the heavy metal tolerance of cucumber plants. Moreover, genomic analysis revealed that the CS51 genome consisted of a circular chromosome of 5,364,174 base pairs with an average G+C content of 64.71%. There were around 4774 predicted protein-coding sequences (CDSs) in 4859 genes, 15 rRNA genes, and 67 tRNA genes. Around 3950 protein-coding genes with function prediction and 733 genes without function prediction were identified. Furthermore, functional analyses predicted that the CS51 genome could encode genes required for auxin biosynthesis, nitrate and nitrite ammonification, the phosphate-specific transport system, and the sulfate transport system, which are beneficial for plant growth promotion. The heavy metal resistance of CS51 was confirmed by the presence of genes responsible for cobalt-zinc-cadmium resistance, nickel transport, and copper homeostasis in the CS51 genome. The extrapolation of the curve showed that the core genome contained a minimum of 2122 genes (95% confidence interval = 2034.24 to 2080.215). Our findings indicated that the genome sequence of CS51 may be used as an eco-friendly bioresource to promote plant growth in heavy metal-contaminated areas.