Andrea J. Grisold
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Previous Organizations / Institutes
The organizations / institutes from the scholar's other articles in the past ten years
Research Subjects
Andrea J. Grisold has a total of 4 co-authors. Here are the top co-authors by number of shared publications:
Harald H. Kessler
Medical University of Graz
2 shared publicationsEgon Marth
Institute of Hygiene, Karl Franzens University Graz, A-8010 Graz,1 Roche Diagnostics GmbH, A-1211 Vienna, Austria2
1 shared publication- Eva Leitner
Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria
1 shared publication Gerhard MühLbauer
Roche Diagnostics GmbH, A-1211 Vienna, Austria
1 shared publication
Latest Publications
- 1 January 2006
- Published by Springer Nature
- Vol. 345, 79-90
Use of Hybridization Probes in a Real-Time PCR Assay on the LightCycler® for the Detection of Methicillin-Resistant Staphylococcus aureus
The rapid and accurate identification of methicillin-resistant Staphylococcus aureus (MRSA) is of great importance for the affected patient, the involved ward, and the microbiological laboratory. Resistance to methicillin is encoded by the mecA gene in S. aureus. Because routine laboratory diagnostics may be time consuming and because species differentiation encounters a variety of difficulties, molecular techniques detecting both the mecA and a S. aureus-specific gene are used for rapid and accurate detection and identification of MRSA. Various protocols, including the manual extraction of DNA have been established. In this chapter, the identification of MRSA based on simultaneous detection of the mecA gene and the S. aureus-specific Sa442 DNA fragment using automated DNA extraction and real-time polymerase chain reaction is described. This method is an attractive alternative to labor-intensive manual protocols and can easily be incorporated into the diagnostic microbiology laboratory workflow, with the ability to obtain results within 4 h.
- 1 January 2006
- Published by Springer Nature
- Vol. 345, 79-90
Use of Hybridization Probes in a Real-Time PCR Assay on the LightCycler® for the Detection of Methicillin-Resistant Staphylococcus aureus
The rapid and accurate identification of methicillin-resistant Staphylococcus aureus (MRSA) is of great importance for the affected patient, the involved ward, and the microbiological laboratory. Resistance to methicillin is encoded by the mecA gene in S. aureus. Because routine laboratory diagnostics may be time consuming and because species differentiation encounters a variety of difficulties, molecular techniques detecting both the mecA and a S. aureus-specific gene are used for rapid and accurate detection and identification of MRSA. Various protocols, including the manual extraction of DNA have been established. In this chapter, the identification of MRSA based on simultaneous detection of the mecA gene and the S. aureus-specific Sa442 DNA fragment using automated DNA extraction and real-time polymerase chain reaction is described. This method is an attractive alternative to labor-intensive manual protocols and can easily be incorporated into the diagnostic microbiology laboratory workflow, with the ability to obtain results within 4 h.
- 1 July 2002
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (7) , 2392-2397
Detection of Methicillin-ResistantStaphylococcus aureusand Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real-Time PCR
A molecular assay for the simultaneous detection of aStaphylococcus aureus-specific gene and themecAgene, responsible for the resistance to methicillin in staphylococci, was evaluated. The assay included an automated DNA extraction protocol conducted with a MagNA Pure instrument and real-time PCR conducted with a LightCycler instrument. The performance and robustness of the assay were evaluated for a suspension of methicillin-resistantS. aureus(MRSA) strain with a turbidity equivalent to a McFarland standard of 0.5, which was found to be the ideal working concentration. The specificity of the new molecular assay was tested with a panel of 30 gram-negative and gram-positive bacterial strains other than MRSA. No cross-reactivity was observed. In a clinical study, 109 isolates of MRSA were investigated. All clinical MRSA isolates gave positive results for theS. aureus-specific genomic target, and all but one were positive for themecAgene. In conclusion, the new molecular assay was found to be quick, robust, and laborsaving, and it proved to be suitable for a routine molecular diagnostic laboratory.
- 1 July 2002
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (7) , 2392-2397
Detection of Methicillin-ResistantStaphylococcus aureusand Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real-Time PCR
A molecular assay for the simultaneous detection of aStaphylococcus aureus-specific gene and themecAgene, responsible for the resistance to methicillin in staphylococci, was evaluated. The assay included an automated DNA extraction protocol conducted with a MagNA Pure instrument and real-time PCR conducted with a LightCycler instrument. The performance and robustness of the assay were evaluated for a suspension of methicillin-resistantS. aureus(MRSA) strain with a turbidity equivalent to a McFarland standard of 0.5, which was found to be the ideal working concentration. The specificity of the new molecular assay was tested with a panel of 30 gram-negative and gram-positive bacterial strains other than MRSA. No cross-reactivity was observed. In a clinical study, 109 isolates of MRSA were investigated. All clinical MRSA isolates gave positive results for theS. aureus-specific genomic target, and all but one were positive for themecAgene. In conclusion, the new molecular assay was found to be quick, robust, and laborsaving, and it proved to be suitable for a routine molecular diagnostic laboratory.