Huaxiang Rao

Brucellosis poses a significant public health challenge. This study aimed to analyze the epidemiological characteristics and spatiotemporal evolution of hu

In this study, HFRS data were obtained from China CDC and ECDC, while monthly meteorological data and GDP were extracted from the National Bureau of Statistics of China website. Descriptive epidemiology, time series decomposition, and spatial autocorrelation analyses were employed to evaluate HFRS incidence patterns. A spatial panel data model was used to estimate the effects of meteorological and socio-economic variables on HFRS incidence. The average annual incidence rate of HFRS was 0.90/100000 in China, compared to 29.3/100000 in Finland. The incidence level in China was comparable to that in Belgium and the EU/EEA (excluding the UK), the high-incidence age group was 45–64 years, which was similar to Finland and the EU/EEA. HFRS in China exhibited marked seasonality. Three north-eastern provinces, Shaanxi, Shandong, and Jiangxi reported higher incidence rates. After adjusting for spatial individual effects and spatial autocorrelation, HFRS incidence was negatively associated with precipitation during the same period, per capita GDP showed no significant effect on HFRS incidence. Continued surveillance and prevention of HFRS remain necessary in China, particularly in Shaanxi. Additional disease prevention and control efforts should be directed towards individuals aged 45–64 years during the high-risk period from October to December.

Neoehrlichia mikurensis is vector-borne gram-negative intracellular bacteria that has a wide range of hosts, and are recognized as emerging human pathogens. This study aimed to investigate the prevalence and genetic characteristics of N. mikurensis infections in rodents from central and southern of Shanxi Province, China. The rodents were captured, and the liver, spleen and kidney were collected for N. mikurensis detection and identification by 16 S rRNA and groEL genes sequencing. N. mikurensis was detected in Apodemus agrarius from Yangquan and Eothenomys inez from Yuncheng, with a positivity rate of 0.66% (2/301). Phylogenetic analysis based on 16 S rRNA and groEL genes revealed that the sequences in this study formed distinct branches separate from reported clusters (Clusters I-IV), clustered near Clusters III and IV, which the pathogenicity was unclear. Analysis of groEL gene polymorphism identified 12 haplotypes (Hap-1 to Hap-12) among 64 sequences (one novel sequence from this study and 63 from GenBank), with high haplotype diversity (Hd = 0.770 ± 0.030). Dominant haplotypes (Hap-2, Hap-3, Hap-4; 82.8% of sequences) exhibited distinct geographic specific patterns. In addition, Asian sequences exhibited greater diversity than European sequences, and human-derived sequences showed the higher genetic diversity than rodent- and tick-derived sequences. Overall, the detection of N. mikurensis in rodents in Shanxi Province expands its known distribution and provides novel insights into the genetic diversity in different regions and hosts.

Background: Babesiosis, a globally emerging tick-borne zoonosis caused by intraerythrocytic protozoan Babesia species, poses a significant threat to both animal and human health. This study investigated the prevalence and genetic diversity of Babesia sp. in small rodents in central and southern Shanxi Province, China. Methods: Rodents were captured from central and southern Shanxi Province, China. Liver, spleen, and kidney specimens were collected and screened for Babesia sp. based on 18S rRNA gene amplification and sequencing. For genetic and evolutionary analysis of Babesia sp. sequences based on the 18S rRNA gene, a phylogenetic tree was created using MEGA 11. Genetic diversity was analyzed using DNASP 6.12.03, and haplotype networks in Babesia microti from different regions and hosts were constructed using PopART software. Results: Three hundred and one rodents were captured; PCR screening revealed a 6.64% (20/301) prevalence of Babesia sp. infection, detected in Niviventer confucianus (16.87%, 14/83) and Apodemus agrarius (3.85%, 6/156). Detection rates did not differ significantly according to sex, tissue, or habitat type. Geographically, central Shanxi exhibited significantly higher detection rates than southern Shanxi (9.74% vs. 0.94%, χ ² = 8.573, P = 0.003). Phylogenetic analysis of the partial 18S rRNA gene (1083 bp) confirmed that all sequences obtained in this study were the B. microti Kobe type, closely related to sequences from southeastern Shanxi obtained in our previous study (with 99.7–100% identity), with the ability to infect humans. Genetic diversity analysis of 65 B. microti sequences from China (20 sequences from the present study and 45 from GenBank) identified 21 haplotypes with host- and geography-specific patterns. Host-specific analysis of 18S rRNA gene polymorphisms revealed higher genetic diversity in tick-derived sequences than in rodent- or human-derived sequences. Haplotype network analysis suggested that Shanxi sequences (Hap-1, Hap-10, and Hap-11) exhibited close genetic proximity of 1–3 nucleotide substitutions with rodent-derived sequences from Yunnan and Fujian provinces and human-derived sequences from Yunnan and Zhejiang provinces. Conclusions: This study found a high prevalence and low genetic diversity of B. microti infection in wild rodents in central Shanxi, which could provide a basis for local corresponding prevention and control strategies. Graphical

Babesia is a tick-transmitted parasite that infects wild and domestic animals, causes babesiosis in humans, and is an increasing public health concern. Here, we investigated the prevalence and molecular characteristics of Babesia infections in the rodents in Southeastern Shanxi, China. Small rodents were captured, and the liver and spleen tissues were used for Babesia detection using traditional PCR and sequencing of the partial 18S rRNA gene. The analysis revealed that 27 of 252 small rodents were positive for Babesia, with an infection rate of 10.71%. The infection rates in different sexes and rodent tissues were not statistically different, but those in different rodent species, habitats, and sampling sites were statistically different. The highest risk of Babesia infection was observed in Niviventer confucianus captured from the forests in Huguan County. Forty-three sequences from 27 small rodents positive for Babesia infection were identified as Babesia microti, including 42 sequences from 26 N. confucianus, and one sequence from Apodemus agrarius. Phylogenetic analysis showed that all sequences were clustered together and had the closest genetic relationship with Babesia microti strains isolated from Rattus losea and N. confucianus in China, and belonged to the Kobe-type, which is pathogenic to humans. Compared to other Kobe-type strains based on the nearly complete 18S rRNA gene, the sequences obtained in this study showed the difference by 1–3 bp. Overall, a high prevalence of Babesia microti infection was observed in small rodents in Southeastern Shanxi, China, which could benefit us to take the implementation of relevant prevention and control measures in this area.

The COVID-19 pandemic has been present globally for more than three years, and cross-border transmission has played an important role in its spread. Currently, most predictions of COVID-19 spread are limited to a country (or a region), and models for cross-border transmission risk assessment remain lacking. Information on imported COVID-19 cases reported from March 2020 to June 2022 was collected from the National Health Commission of China, and COVID-19 epidemic data of the countries of origin of the imported cases were collected on data websites such as WHO and Our World in Data. It is proposed to establish a prediction model suitable for the prevention and control of overseas importation of COVID-19. Firstly, the SIR model was used to fit the epidemic infection status of the countries where the cases were exported, and most of the r2 values of the fitted curves obtained were above 0.75, which indicated that the SIR model could well fit different countries and the infection status of the region. After fitting the epidemic infection status data of overseas exporting countries, on this basis, a SIR-multiple linear regression overseas import risk prediction combination model was established, which can predict the risk of overseas case importation, and the established overseas import risk model overall P <0.05, the adjusted R2 = 0.7, indicating that the SIR-multivariate linear regression overseas import risk prediction combination model can obtain better prediction results. Our model effectively estimates the risk of imported cases of COVID-19 from abroad.

Multistage stratified random sampling was used to explore the relationship of health literacy with novel coronavirus disease 2019 (COVID-19) prevention and control knowledge, attitude and practice (KAP) in residents aged 15–69 years old in Shanxi Province. The questionnaire, which was issued by the Chinese Center for Health Education, consisted of a health literacy questionnaire and a COVID-19 prevention and control KAP questionnaire. According to the national unified scoring method, the participants were divided into two groups: those who with adequate health literacy and those who with inadequate health literacy. The results of the answer to each KAP question were compared between the two groups by Chi-square test or Wilcoxon rank sum test. Binary logistic regression was used to control confounding effects of socio-demographic characteristics to draw relatively reliable conclusions. A total of 2700 questionnaires were distributed, and 2686 valid questionnaires were returned, with an efficiency rate of 99.5%. Health literacy qualified was identified for 18.32% (492/2686) in Shanxi Province. Compared with the inadequate health literacy group, people with adequate health literacy had a higher corrected answer rate in 11 knowledge-related questions (all P < 0.001); showed more positive answer to each attitude-related question in the three aspects, namely, responsibility for the prevention and control of infectious disease transmission, evaluation for COVID-19-related information release and reporting, and evaluation for the government’s COVID-19 prevention and control results (all P < 0.001); and acted more actively in the practice concerning appropriate self-prevention and control behaviors during the COVID-19 outbreak (all P < 0.001). Logistic regression analyses confirmed that with adequate health literacy played a positive role in each of the contents of COVID-19 prevention and control KAP (ORs were between 1.475 and 4.862, all P < 0.001). Health literacy is closely related to COVID-19 prevention and control KAP in the general population of Shanxi Province. People with high score of health literacy were generally better able to grasp COVID-19 prevention and control knowledge, have more positive attitudes toward prevention and control, and perform better prevention and control behaviors. Promoting residents’ health literacy by targeted health education can play an important and positive role in dealing with the threat of major infectious diseases outbreaks.

The aim of this study was to investigate the occurrence and molecular characteristics of Bartonella infections in small rodents in the Shangdang Basin, China. Small rodents were captured using snap traps, and their liver, spleen, and kidney tissues were harvested for Bartonella detection and identification using a combination of real-time PCR of the ssrA gene (296 bp) and conventional PCR and sequencing of the gltA gene (379 bp). Results showed that 55 of 147 small rodents to be positive for Bartonella, with a positivity rate of 37.41%, and 95% confidence interval of 29.50%- 45.33%. While the positivity rate across genders (42.62% in males and 33.72% in females, χ² = 1.208, P = 0.272) and tissues (28.57% in liver, 33.59% in spleen, and 36.76% in kidney, χ² = 2.197, P = 0.333) of small rodents was not statistically different, that in different habitats (5.13% in villages, 84.44% in forests, and 54.17% in farmlands, χ² = 80.105, PBartonella sequences identified in six species, including 30 B. grahamii, three B. phoceensis, two B. japonica, two B. queenslandensis, one B. fuyuanensis and four unknown Bartonella species from Niviventer confucianus, Apodemus agrarius and Tscherskia triton. In addition to habitat, Bartonella species infection could be affected by the rodent species as well. Among the Bartonella species detected in this area, B. grahamii was the dominant epidemic species (accounting for 71.43%). B. grahamii exhibited four distinct clusters, and showed a certain host specificity. In addition, 11 haplotypes of B. grahamii were observed using DNASP 6.12.03, among which nine haplotypes were novel. Overall, high occurrence and genetic diversity of Bartonella were observed among small rodents in the Shangdang Basin; this information could potentially help the prevention and control of rodent-Bartonella species in this area.

In this study, we aimed to investigate the prevalence and molecular characteristics of Bartonella infections in small mammals from the central region of the Qinghai-Tibetan Plateau. Toward this, small mammals were captured using snap traps in Yushu City and Nangqian County, West China, and the spleen tissue was used for Bartonella culture. The suspected positive colonies were evaluated using polymerase chain reaction (PCR) amplification and by sequencing the citrate synthase (gltA) gene. We discovered that 31 out of the 103 small mammals tested positive for Bartonella, with an infection rate of 30.10%. Sex differences between the mammals did not result in a significant difference in infection rate (χ² = 0.018, P = 0.892). However, there was a significant difference in infection rates in different small mammals (Fisher’s exact probability method, P = 0.017) and habitats (χ² = 7.157, P = 0.028). Additionally, 31 Bartonella strains belonging to three species were identified, including B. grahamii (25), B. japonica (4) and B. heixiaziensis (2), among which B. grahamii was the dominant epidemic strain (accounting for 80.65%). Phylogenetic analyses showed that most of the B. grahamii isolates identified in this study may be closely related to the strains isolated from Japan and China. Genetic diversity analyses revealed that B. grahamii strains had high genetic diversity, which showed a certain host and geographical specificity. The results of Tajima’s test suggested that the B. grahamii followed the progressions simulated by a neutral evolutionary model in the process of evolution. Overall, a high prevalence and genetic diversity of Bartonella infection were observed in small mammals in the central region of the Qinghai-Tibetan Plateau. B. grahamii as the dominant epidemic strain may cause diseases in humans, and the corresponding prevention and control measures should be taken into consideration in this area.

The prevalence and molecular characteristics of Bartonella infections in small rodents in the Zhongtiao Mountain, China have been explored. In this study, the liver, spleen and kidney tissues of captured rodents were used for Bartonella spp. detection and identification by combination of real-time PCR of transfer-mRNA (ssrA) gene and traditional PCR and sequencing of citrate synthase (gltA) gene. It was shown that 49.52% of the rodents (52/105) were positive for Bartonella spp.. The infection rate in different gender (χ² = 0.079, P = 0.778) and tissues (χ² = 0.233, P = 0.890) of small rodents did not have statistical difference, but that in different small rodents (Fisher’s exact test, P < 0.001) and habitats (χ² = 5.483, P = 0.019) had statistical difference. And, the sequencing data suggests that Bartonella sequences (n = 31) were identified into three species, including 14 of B. grahamii, 3 of B. queenslandensis and 14 of unknown Bartonella species. Phylogenetic analysis showed that B. grahamii sequences were clustered with the isolates from South Korea and China, and B. queenslandensis sequences were mainly closely related to the isolates from China and Thailand. The genetic diversity analysis showed that B. grahamii and B. queenslandensis sequences exhibited noticeable intraspecies diversity. Taken together our data demonstrates the high prevalence and genetic diversity of Bartonella infections in small rodents in the Zhongtiao Mountain, especially a potential novel Bartonella specie was detected, which could benefit the prevention and control of rodent-Bartonella species in this area.