Toshio Takayama
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Toshio Takayama has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Chia-Hung Dylan Tsai
Department of Photonics, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan
1 shared publication - Hiroaki Ito
Department of Physics Chiba University 263-8522 Chiba Japan
1 shared publication - Makoto Kaneko
Department of Mechanical Engineering, Osaka University, Suita 565-0871, Japan
1 shared publication - Mitsuhiro Horade
Department of Mechanical Engineering, Osaka University, Suita 565-0871, Japan
1 shared publication - Nobuya Higashino
Department of Mechanical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
1 shared publication - Yasutaka Yamaguchi
Department of Mechanical Engineering, the University of Osaka 2 , 2-1 Yamadaoka, Suita 565-0871
1 shared publication
Latest Publications
- Open Access
LED-CT Scan for pH Distribution on a Cross-Section of Cell Culture Medium
In cell culture, the pH of the culture medium is one of the most important conditions. However, the culture medium may have non-uniform pH distribution due to activities of cells and changes in the environment. Although it is possible to measure the pH distribution with an existing pH meter using distributed electrodes, the method involves direct contact with the medium and would greatly increase the risk of contamination. Here in this paper, we propose a computed tomography (CT) scan for measuring pH distribution using the color change of phenol red with a light-emitting diode (LED) light source. Using the principle of CT scan, we can measure pH distribution without contacting culture medium, and thus, decrease the risk of contamination. We have developed the device with a LED, an array of photo receivers and a rotation mechanism. The system is firstly calibrated with different shapes of wooden objects that do not pass light, we succeeded in obtaining their 3D topographies. The system was also used for measuring a culture medium with two different pH values, it was possible to obtain a pH distribution that clearly shows the boundary.
Open AccessLED-CT Scan for pH Distribution on a Cross-Section of Cell Culture Medium
In cell culture, the pH of the culture medium is one of the most important conditions. However, the culture medium may have non-uniform pH distribution due to activities of cells and changes in the environment. Although it is possible to measure the pH distribution with an existing pH meter using distributed electrodes, the method involves direct contact with the medium and would greatly increase the risk of contamination. Here in this paper, we propose a computed tomography (CT) scan for measuring pH distribution using the color change of phenol red with a light-emitting diode (LED) light source. Using the principle of CT scan, we can measure pH distribution without contacting culture medium, and thus, decrease the risk of contamination. We have developed the device with a LED, an array of photo receivers and a rotation mechanism. The system is firstly calibrated with different shapes of wooden objects that do not pass light, we succeeded in obtaining their 3D topographies. The system was also used for measuring a culture medium with two different pH values, it was possible to obtain a pH distribution that clearly shows the boundary.