Cristina Arce

One of the factors involved in goat milk production is the role of women as farmers. The aim of this study was to evaluate the role of women on dairy goat farms, considering: (1) the profile of women occupationally involved, (2) the organization of the women’s work, (3) the degree of involvement by women in the decision-making on these farms, and (4) the influence of women’s work on productive results. This study was conducted on 52 dairy goat farms in southern Spain. A descriptive analysis and means comparisons were performed to describe the farms where any women were involved or not. In 61.5% of the farms, at least one woman was involved, with an age of 42.2 ± 8.8 years. Very few women were farm owners, although women took binding decisions in 81.25% of these farms. Their work is dedicated to milking and caring for the kids. Women had a positive influence on the productive variables analysed, and for mastitis in herds, the incidence was lower in herds where women participated (p < 0.01). In conclusion, it is recommended to include women’s work as a factor when characterizing dairy goats farms’ systems to evaluate their positive effect on a farm’s performance.

The main objective of this study was to evaluate the effect of the production system and other environmental/phenotype factors on age at first kidding (AFK), kidding interval (KI) and prolificacy of 19,772 Florida goats reared between 2000 and 2019 on 49 dairy farms (38 farms intensively managed and 11 extensively managed with grazing). AFK was lower on intensive (490.2 ± 0.9 days; n = 13,345) than on extensive farms (511.7 ± 2.5 days; n = 2357; p < 0.001), and highest during the spring season (533.9 ± 2.7 days; n = 1932; p < 0.001) in both production systems. The average KI was 355.7 ± 0.4 days, mainly varying according to dry period, kidding season and lactation number and kidding type (p < 0.01). A significant interaction between production system, kidding season and dry period was observed with the highest AFK on intensive farms during spring and summer for goats presenting a dry period of up to six months. The overall prolificacy (1.64 ± 0.01) increased in recent years in both systems, and it was affected by the production system, but with different patterns; so, the highest prolificacy of primiparous and multiparous goats was observed on extensive and intensive farms, respectively. Besides that, the prolificacy and other reproductive parameters, such as AFK, significantly increased in the last decade, which could be related to management improvements. Besides that, the existence of inter-annual variations should be considered to compare data between farms and years, and to establish the farms’ objectives according to their production systems and production goals.

Infected pork meat is an important source of non-typhoidal human salmonellosis. Understanding of molecular mechanisms involved in disease pathogenesis is important for the development of therapeutic and preventive strategies. Thus, hereby we study the transcriptional profiles along the porcine intestine during infection with Salmonella Typhimurium, as well as post-transcriptional gene modulation by microRNAs (miRNA). Sixteen piglets were orally challenged with S. Typhimurium. Samples from jejunum, ileum and colon, collected 1, 2 and 6 days post infection (dpi) were hybridized to mRNA and miRNA expression microarrays and analyzed. Jejunum showed a reduced transcriptional response indicating mild inflammation only at 2 dpi. In ileum inflammatory genes were overexpressed (e.g., IL-1B, IL-6, IL-8, IL1RAP, TNFα), indicating a strong immune response at all times of infection. Infection also down-regulated genes of the FXR pathway (e.g., NR1H4, FABP6, APOA1, SLC10A2), indicating disruption of the bile acid absorption in ileum. This result was confirmed by decreased high-density lipoprotein cholesterol in serum of infected pigs. Ileal inflammatory gene expression changes peaked at 2 dpi and tended to resolve at 6 dpi. Furthermore, miRNA analysis of ileum at 2 dpi revealed 62 miRNAs potentially regulating target genes involved in this inflammatory process (e.g., miR-374 and miR-451). In colon, genes involved in epithelial adherence, proliferation and cellular reorganization were down-regulated at 2 and 6 dpi. In summary, here we show the transcriptional changes occurring at the intestine at different time points of the infection, which are mainly related to inflammation and disruption of the bile acid metabolism.

The enteropathogen Salmonella Typhimurium (S. Typhimurium) is the most commonly nontyphoideal serotype isolated in pig worldwide. Currently, one of the main sources of human infection is by consumption of pork meat. Therefore, prevention and control of salmonellosis in pigs is crucial for minimizing risks to public health. The aim of the present study was to use isobaric tags for relative and absolute quantification (iTRAQ) to explore differences in the response to Salmonella in two segment of the porcine gut (ileum and colon) along a time course of 1, 2 and 6 days post infection (dpi) with S. Typhimurium. A total of 298 proteins were identified in the infected ileum samples of which, 112 displayed significant expression differences due to Salmonella infection. In colon, 184 proteins were detected in the infected samples of which 46 resulted differentially expressed with respect to the controls. The higher number of changes in protein expression was quantified in ileum at 2 dpi. Further biological interpretation of proteomics data using bioinformatics tools demonstrated that the expression changes in colon were found in proteins involved in cell death and survival, tissue morphology or molecular transport at the early stages and tissue regeneration at 6 dpi. In ileum, however, changes in protein expression were mainly related to immunological and infection diseases, inflammatory response or connective tissue disorders at 1 and 2 dpi. iTRAQ has proved to be a proteomic robust approach allowing us to identify ileum as the earliest response focus upon S. Typhimurium in the porcine gut. In addition, new functions involved in the response to bacteria such as eIF2 signalling, free radical scavengers or antimicrobial peptides expression have been identified. Finally, the impairment at of the enterohepatic circulation of bile acids and lipid metabolism by means the under regulation of FABP6 protein and FXR/RXR and LXR/RXR signalling pathway in ileum has been established for the first time in pigs. Taken together, our results provide a better understanding of the porcine response to Salmonella infection and the molecular mechanisms underlying Salmonella-host interactions.

Background: Once a list of differentially expressed genes has been identified from a microarray experiment, a subsequent post-analysis task is required in order to find the main biological processes associated to the experimental system. This paper describes two pathways analysis tools, ArrayUnlock and Ingenuity Pathways Analysis (IPA) to deal with the post-analyses of microarray data, in the context of the EADGENE and SABRE post-analysis workshop. Dataset employed in this study proceeded from an experimental chicken infection performed to study the host reactions after a homologous or heterologous secondary challenge with two species of Eimeria. Results: Analysis of the same microarray data source employing both commercial pathway analysis tools in parallel let to identify several biological and/or molecular functions altered in the chicken Eimeria maxima infection model, including several immune system related pathways. Biological functions differentially altered in the homologous and heterologous second infection were identified. Similarly, the effect of the timing in a homologous second infection was characterized by several biological functions. Conclusion: Functional analysis with ArrayUnlock and IPA provided information related to functional differences with the three comparisons of the chicken infection leading to similar conclusions. ArrayUnlock let an improvement of the annotations of the chicken genome adding InterPro annotations to the data set file. IPA provides two powerful tools to understand the pathway analysis results: the networks and canonical pathways that showed several pathways related to an adaptative immune response.

A monoclonal antibody (JM2E5) specific for the integrin β₃ chain, or CD61 or GPIIIa subunit, has been employed to determine the expression of the canine homologue CD41/CD61 or CD51/CD61 complex on different canine cells in peripheral blood lymphocytes, monocytes, granulocytes, platelets, erythrocytes, lymph-node cells, spleen cells and breast tumour cells). The canine homologue CD41/CD61 or CD51/61 was present on peripheral blood lymphocytes, monocytes, granulocytes, breast tumour cells and spleen cells as well as on platelets and it was absent from erythrocytes and lymph-node cells. An antigen with components of molecular masses of 25/100/120 kDa (under reducing conditions) was immunoprecipitated from canine peripheral lymphocytes and platelets, but not from granulocytes or monocytes. Expression on canine lymphocytes of the canine homologue of the human β₃ integrin chain was unexpected, based on the expression pattern of this molecule in human tissue.