Rita Pinto Proença

In just one hundred years, the world has been through five pandemics. Lessons of the past have not prevented a new virus from being responsible for a fifth wave of deaths worldwide, even with scientific advances and the rapid response of societies that, for the most part, anticipated the political and economic response. A century after the end of the Spanish Flu (1918/19), in December 2019, the world has watched the beginning of the second pandemic of the 21st century, coronavirus disease 2019 (COVID-19) caused by a new severe acute respiratory coronavirus syndrome-2 (SARS-CoV-2).The eye can not only be the entry point for coronaviruses, but also a target organ. Conjunctivitis, uveitis, vasculitis, retinitis and optic neuritis have all been documented in animal models studies.In this article, we review the possible roles of the visual system/ocular tissues as an entryway, and the potential ophthalmic manifestations of human SARS-CoV-2 infections. This review article will also highlight the most effective ways for protecting and preventing the spread of the virus.

Internal carotid artery dissection (ICAD) is caused by the disruption of the tunica intima, with the formation of an intramural haematoma that can cause stenosis or occlusion of the artery’s lumen, leading to reduced blood flow and secondary thrombus formation. Up to two-thirds of patients with ICAD show ophthalmological symptoms or signs, which are, frequently, the first manifestations of this clinical condition, often preceding for weeks the neurological signs of cerebral infarction. Central retinal artery occlusion (CRAO) is a rare complication of ICAD, secondary either to haemodynamic compromise, with ocular hypoperfusion and reverse flow within the ophthalmic artery, or to thromboembolic events, in rarer cases. We report a case of CRAO secondary to a spontaneous ICAD, in an otherwise healthy middle-aged patient.

Leber’s hereditary optic neuropathy (LHON) is an optic neuropathy of mitochondrial inheritance, characterised by incomplete penetrance and variable expressivity. Typically, young male patients present with sequential, severe, rapidly progressive loss of central vision, with characteristic funduscopic findings. However, LHON may present at any age, in both genders, and fundus examination may be normal. Evidence has emerged to support the role of environmental factors in triggering LHON, by disrupting the normal mechanisms of mitochondrial function. We present two clinical cases of LHON of late onset, and provide a literature review on atypical cases of LHON and the role of environmental triggers.

The Rho kinase (ROCK) signaling pathway is involved in several cellular events that include cell proliferation and cytoskeleton modulation leading to cell adhesion. The ROCK pathway in the human eye has been hypothesized to play important roles in corneal endothelial cell physiology and pathologic states. In addition, ROCK signaling has been identified as an important regulator of trabecular meshwork (TM) outflow, which is altered in glaucomatous eyes. These roles in corneal and glaucomatous disease states have led to the growing interest in the development of drugs selectively targeting this pathway (ROCK inhibitors). The authors provide a review of the literature on the pathobiology of the ROCK signaling in corneal endothelial disease, glaucoma, and vitreoretinal disease, as well as the clinical usefulness of ROCK inhibitors in Ophthalmology.

The authors report a case of an orbital metastasis from an occult breast carcinoma. A 66-year-old woman presented with a growing left orbital tumour. Orbital CT scan was consistent with lymphoma. However, ocular pathology revealed small neoplastic cells showing an ‘indian file pattern’ suggestive of metastatic carcinoma and immunohistochemistry was positive for CK7, CK CAM5.2 and oestrogen receptor. A systemic evaluation was then performed with mammogram, breast ultrasound and MRI considered normal. An exhaustive systemic evaluation revealed multiple bone lesions, a right axillary lymph node lesion, which presented the same pattern on pathology and immunohistochemistry, with no evidence of a primary tumour. A diagnosis of a metastatic lobular carcinoma of the breast (T0, N1, M1) was made and the patient was started on chemotherapy and adjuvant hormonal therapy.

Purpose: To compare choroidal thickness (CT) between diabetic patients without diabetic retinopathy and a nondiabetic group. To explore how CT relates to disease duration, mean arterial pressure, glycemia, glycosylated hemoglobin, intraocular pressure, and ocular pulse amplitude. Methods: Choroidal thickness was assessed using a spectral-domain optical coherence tomography and enhanced depth mode at 13 locations (subfoveal and 3 measurements 500 μm apart in 4 directions—nasal, temporal, superior, and inferior). Linear regression models were used. Results: One hundred seventy-five patients were recruited (125 diabetic patients without diabetic retinopathy and 50 nondiabetic patients). In diabetic patients, although without statistical significance, CT showed a trend to be thicker in all locations (6.16–24.27 μm). Choroidal thickness was negatively associated with age (P < 0.001) in both groups, but only in the diabetic group, it was positively associated to ocular pulse amplitude (with a mean increase between 8.5 μm and 11.6 μm for each millimeter of mercury increase in ocular pulse amplitude). Diabetic patients' CT seems to stabilize after 150 months of diabetes, increase with higher glycemia levels (>160 mg/dL) while showing no fluctuation with glycosylated hemoglobin and mean arterial pressure. Conclusion: There seems to be a thickening of the choroid in diabetic patients without diabetic retinopathy. Moreover, this tissue may be functionally different in diabetes, as the pattern of associations seems to differ between groups.

Conjunctival melanoma is a rare but potentially lethal tumor. Its biologic profile is still largely unknown, with recent studies aiming at establishing histopathological and genetic tumor profiles. The aim of this study was to analyze the association between clinicopathological characteristics and tumor expression of cyclooxygenase-2 (COX-2) to prognosis, assessing its usefulness as a possible prognostic marker.

Purpose: Conjunctival melanoma is a rare but potentially lethal tumour. Its biologic profile is still largely unknown, with recent studies aiming at establishing histopathological and genetic tumour profiles. The aim of this study was to analyse the association between clinicopathological characteristics and tumour expression of cyclooxygenase‐2 (COX‐2) to prognosis, assessing its usefulness as a possible prognostic marker.Methods: Case series of 50 patients from 1991 to 2008 with pathologically proven conjunctival melanoma. Demographic, clinical and pathological characteristics were evaluated by reviewing clinical files and pathology. Expression of COX‐2 was studied by immunohistochemistry of formalin‐fixed paraffin‐embedded tissue samples of 20 melanomas. Samples were classified in a score which included intensity of staining and percentage of cells with positive reactivity.Results: Clinicopathological features significantly associated (p < .05) with a poor prognosis (death) included involvement of fornix and tarsal conjunctiva, tumour thickness exceeding 2 mm, local tumour recurrence, lymph node and systemic metastasis. In the immunohistochemistry study (n = 20) all cases expressed COX‐2 although with different scores. However, only cases with score 4 were associated with a poor outcome. Multivariate association analysis revealed that recurrence rate, metastasis, corneal invasion and tumour thickness were associated with score 4 cases and, therefore, with a clinical profile with a higher risk of death.Conclusions: Results suggest that higher COX‐2 expression may be a negative prognostic factor in conjunctival melanoma. Further studies can address the potential use of anti‐COX‐2 drugs as adjuvant therapy of this disease.