Margaret Hauser
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Margaret Hauser has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Ke Xu
University of California, Berkeley
2 shared publications - Wan Li
Department of Chemistry
2 shared publications Michal Wojcik
Department of Chemistry, University of California, Berkeley, California 94720, USA
1 shared publicationSamuel J Kenny
Department of Chemistry, University of California, Berkeley, Berkeley, California, USA
1 shared publicationSeonah Moon
Department of Chemistry, University of California, Berkeley, California 94720, USA
1 shared publicationZhengyang Zhang
Department of Chemistry, University of California, Berkeley, Berkeley, California, USA
1 shared publication
Latest Publications
- 17 August 2015
- Published by Springer Nature in Nature Methods
- Vol. 12 (10) , 935-938
Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy
By developing a wide-field scheme for spectral measurement and implementing photoswitching, we synchronously obtained the fluorescence spectra and positions of ∼10(6) single molecules in labeled cells in minutes, which consequently enabled spectrally resolved, 'true-color' super-resolution microscopy. The method, called spectrally resolved stochastic optical reconstruction microscopy (SR-STORM), achieved cross-talk-free three-dimensional (3D) imaging for four dyes 10 nm apart in emission spectrum. Excellent resolution was obtained for every channel, and 3D localizations of all molecules were automatically aligned within one imaging path.
- 17 August 2015
- Published by Springer Nature in Nature Methods
- Vol. 12 (10) , 935-938
Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy
By developing a wide-field scheme for spectral measurement and implementing photoswitching, we synchronously obtained the fluorescence spectra and positions of ∼10(6) single molecules in labeled cells in minutes, which consequently enabled spectrally resolved, 'true-color' super-resolution microscopy. The method, called spectrally resolved stochastic optical reconstruction microscopy (SR-STORM), achieved cross-talk-free three-dimensional (3D) imaging for four dyes 10 nm apart in emission spectrum. Excellent resolution was obtained for every channel, and 3D localizations of all molecules were automatically aligned within one imaging path.
- Open Access
- 11 June 2015
- Published by Springer Nature in Nature Communications
- Vol. 6 (1) , 7384
Graphene-enabled electron microscopy and correlated super-resolution microscopy of wet cells
The application of electron microscopy to hydrated biological samples has been limited by high-vacuum operating conditions. Traditional methods utilize harsh and laborious sample dehydration procedures, often leading to structural artefacts and creating difficulties for correlating results with high-resolution fluorescence microscopy. Here, we utilize graphene, a single-atom-thick carbon meshwork, as the thinnest possible impermeable and conductive membrane to protect animal cells from vacuum, thus enabling high-resolution electron microscopy of wet and untreated whole cells with exceptional ease. Our approach further allows for facile correlative super-resolution and electron microscopy of wet cells directly on the culturing substrate. In particular, individual cytoskeletal actin filaments are resolved in hydrated samples through electron microscopy and well correlated with super-resolution results.
- 11 June 2015
- Published by Springer Nature in Nature Communications
- Vol. 6 (1) , 7384
Open AccessGraphene-enabled electron microscopy and correlated super-resolution microscopy of wet cells
The application of electron microscopy to hydrated biological samples has been limited by high-vacuum operating conditions. Traditional methods utilize harsh and laborious sample dehydration procedures, often leading to structural artefacts and creating difficulties for correlating results with high-resolution fluorescence microscopy. Here, we utilize graphene, a single-atom-thick carbon meshwork, as the thinnest possible impermeable and conductive membrane to protect animal cells from vacuum, thus enabling high-resolution electron microscopy of wet and untreated whole cells with exceptional ease. Our approach further allows for facile correlative super-resolution and electron microscopy of wet cells directly on the culturing substrate. In particular, individual cytoskeletal actin filaments are resolved in hydrated samples through electron microscopy and well correlated with super-resolution results.