João César Zielak

Introduction: In regenerative medicine, there is a growing demand for innovative strategies to treat difficult-to-heal cutaneous wounds

Introduction: Umbilical cord-derived mesenchymal stem cells, especially from Wharton jelly (WJ-MSCs), are a promising source for regenerative medicine due to their low immunogenicity, immunomodulatory properties, and ethical sourcing

Aim: High heterogeneity in ligature and repair models might reflect different disease status and preclude comparisons. This study aimed to characterize time-dependent alveolar bone loss, histological changes, and immunoinflammatory profiles in rats during ligature-induced periodontitis progression and repair in the long term. Methods: Forty-eight male Wistar rats were included. The negative control group (n=8) received no ligature, while experimental groups (Ligature and Repair; n=40) received a cotton ligature around the lower first molars, bilaterally. After 7d (baseline), ligatures were either maintained or removed for another 28 or 56 days (n=8/group). Hemi-mandibles were collected for radiographic, histological and stereometric analyses. Serum and gingival tissues were collected for the analysis of inflammatory markers using multiplex. Results: Mean bone loss progressively increased in ligated rats (p<0.05). Ligature placement increased the percentage of inflammatory cells at all timepoints and blood vessels at 7 and 35 days, while reducing fibroblasts and extracellular matrix (p<0.05). After 56 days of ligature removal, radiographic bone loss increased compared to 28 days (p<0.05). Ligature removal significantly decreased the percentage of inflammatory cells and blood vessels, while increasing fibroblasts and extracellular matrix only at 56 days. Immunological assay revealed significant time-dependent alterations in serum and gingival markers during disease progression and repair. Conclusion: Ligatures led to cumulative alveolar bone loss and heightened pro-inflammatory cytokines, with distinct inflammatory profiles over time. During repair, despite reducing inflammatory cells and cytokines, bone loss increased in the long term. Our data can assist in designing future experiments and clarify previously published findings.

Oral manifestations following COVID-19 infection can cause discomfort to patients, therefore new treatments must be investigated in order to avoid or minimize this condition. The aim of this study was to report on the use of oxygen therapy in the treatment of COVID-19 oral manifestations. A 22-year-old male patient sought dental care 15 days after isolation due to the infection. He noticed that lesions had arisen in his oral cavity, having initially decreased, but returned after quarantine. The patient had difficulty in eating due to painful symptoms and a burning sensation in the tongue. During clinical examination several lesions were found in his mouth. Treatment using oxygen therapy was proposed with the mouthwash containing active oxygen. After the first day of treatment the patient reported that he was able to eat more easily; after the second day he was able to eat normally, and the burning sensation in his tongue also stopped; by the tenth day total lesions regression had occurred. It was found that the use of oxygen therapy promotes healing of oral lesions due to COVID-19, bringing comfort to the patient, reducing painful symptoms and leading to total healing of lesions.

ATENÇÃO: Os registros DOI estão sendo ativados gradativamente. Até o dia 30/08 todos estarão funcionais. Caso deseje inserir a publicação no ...

O objetivo deste trabalho foi realizar um levantamento transversal descritivo com base em exames radiográficos e informações dos prontuários de pacientes submetidos a instalação de implantes Cone Morse Screw Platinum® em uma clínica privada na cidade de Curitiba, PR, Brasil. Foram avaliados os prontuários e os exames radiográficos dos pacientes com idade igual ou superior a 18 anos, de ambos os sexos, atendidos no período de fevereiro de 2015 e junho de 2019. Foram incluídos apenas aqueles com tempo mínimo de instalação de seis meses e que s prontuários estivessem completos. Os dados coletados foram: sexo (masculino ou feminino), idade (em anos), tempo que o implante estava instalado (em meses), arcada (maxila ou mandíbula) e região (anterior ou posterior), posição do implante em relação à altura da crista óssea marginal (infraósseos, em nível ósseo, ou supraósseos). Um total de 27 pacientes (13 homens e 14 mulheres) e 131 implantes foram avaliados. A mediana da idade foi 59 anos e de tempo de instalação do implante foram 15 meses. Quanto à arcada, 70,2% estavam em maxila e a região posterior teve frequência de 63,3%. Um total de 88 implantes estava em posição infraóssea. Pode-se concluir que a maioria dos implantes avaliados estava, em relação à crista óssea marginal, em posição infraóssea, que é considerada a ideal. A maxila, tanto em região anterior como posterior foi a mais reabilitada, o que pode indicar a importância da questão estética por parte dos pacientes aqui analisados.

Aim: The aim of the present study was to evaluate the microbial contamination in internal and external walls of cone morse implant walls. Methods: Eleven patients with edentulous mandibular posterior area were selected to received dental implants, divided into groups: submerged (S), non-submerged (NS), and immediately loaded (IL). Microbiological evaluations (microorganisms’ number, aerobic and anaerobic colony forming units (CFU) number and microorganisms’ qualification) were divided into internal and external collection of the implant walls, at different stages: T0 (surgical procedure), T2 (suture removal), T4 (reopening S group), T6 (suture removal S group), and T8 (abutment placement in S and NS). All data were submitted to statistical analyses, with confidence level of 0.05. Results: There was difference in number of microorganisms observed over time within the same group (p < 0.05). A difference was observed in CFU when evaluated within the same group over time (p < 0.05), except for the IL group. In internal collection, a predominance of non-formation of microorganisms was observed at T0 in all groups, while formation of Gram-positive Diplococci and Gram-positive Bacilli was observed at T8 (p>0.05). In external collection, an increase in number of microorganisms was observed at T0. Conclusion: There was no difference in microbial contamination among the evaluated groups. The microorganism’s colonization changed over time.

The scaffolds and their interaction with mesenchymal stem cells are objects of study in bioengineering and tissue repair. Mechanisms such as surface adhesion, proliferation, viability, and cytotoxicity are essential for the development of therapies. The present study analyzed the influence of platelet-rich fibrin (PRF) in viability, cytotoxicity, and proliferation of stem cells from human exfoliated deciduous teeth (SHED) exposed to bovine biomaterial surfaces. The studied groups were divided and analyzed as follows: (S) only SHED as control Group; (SB) SHED + biomaterial; (SBP) SHED + biomaterial + PRF. Analyses of cells seeded in 24-well plates were performed after 24, 48 and 72 hours. Individual groups were subjected to viability, cytotoxicity and cell proliferation tests using neutral red, MTT and crystal violet, respectively; and in the 72-hour group, scanning electron microscopy (SEM) was performed to record cell ultra-morphology. Data were submitted to statistical analysis by two-factor ANOVA with a significance level of 5%. The results demonstrated a better performance in the viability/cytotoxicity and proliferation of stem cells for the group (SBP) in comparison to the group (SB) and the group (S). The applied statistical tests showed that the biomaterial factor, time, and interaction between them gave rise to results with statistical significance. SHED submitted to bovine biomaterial were more viable, proliferative and with lower toxicity when associated with PRF. PRF seemed to activate the metabolism of stem cells in culture, indicating that such an association can bring an effective benefit in clinical outcome.