Donát Magyar

Yeasts and yeast-like fungi represent an understudied component of indoor mycobiota, despite including numerous opportunistic human pathogens. This study investigated the diversity, habitats, and potential dispersal mechanisms of yeasts in non-clinical indoor environments. A review of previous studies was combined with new field and laboratory observations. Overall, 1977 yeast occurrence records, including 106 records generated during this study, were analyzed. In total, 83 genera comprising 170 species were identified from 1320 habitat records. Most frequently reported species were Candida parapsilosis (13.8%), Rhodotorula mucilaginosa (7.7%), and Exophiala dermatitidis (7.1%). Habitat records were dominated by wet-cell environments (90.9%), particularly dishwashers, sinks, plumbing systems, tap water and washing machines. Additional habitats included indoor plants, potting soils and wooden materials, the latter representing a previously unreported indoor habitat. Multiple correspondence analysis identified moisture availability as the principal factor structuring habitat associations. Among 89 isolates, ballistospore production, long filamentous thread formation, and dry aerial structures were observed in 3.3%, 2.2%, and 16.9%, respectively. In E. dermatitidis, long filamentous threads were observed to facilitate dispersal by both air currents and contact with surfaces, as confirmed experimentally in one of the investigated strains. These findings expand knowledge of indoor yeast ecology and suggest broader dispersal strategies than previously recognized, while highlighting the need for further ecological and epidemiological studies.

Ambrosia artemisiifolia (ragweed) is a highly invasive species that produces large amounts of allergenic pollen. This poses a serious health risk to allergy sufferers. The “Ragweed Finder” is an Austrian citizen science platform (website and app) that enables the public to report occurrences of ragweed, which are then verified by experts. Over 90% of reports are confirmed as positive, with most originating from eastern Austria, where ragweed is widespread. The number of reports has generally increased over time, except in 2020 during the pandemic. Report frequency does not directly correlate with daily pollen concentrations, but peaks before and during pollen season. Most observations occur along traffic routes, likely due to seed dispersal by vehicle airflow or easier accessibility for users. Verified observations are displayed on an interactive map, helping allergy sufferers to avoid exposure and informing local authorities of the need for targeted control actions. The data are also used to raise awareness among policymakers and help to enact the first law for the control and prevention of ragweed in Burgenland (Austria), in 2021: the “Burgenland Ragweed Control Act”. This demonstrates the success of the “Ragweed Finder” as an important tool for monitoring this invasive species in Austria.

The quality of indoor air is dependent on a number of factors, including the presence of microorganisms that colonize the building materials. The potential for health risks associated with microbial contamination is a significant concern during the renovation of buildings. The aim of this study was to assess the impact of two reconstruction methods for historic buildings on air quality. The two reconstruction procedures were facadism, which preserves only the façade, demolishing the rest of the building and constructing a new building, and complete reconstruction, which involves internal renovation with a less intensive demolition. A total of 70 + 70 air samples, as well as surface and dust samples, were collected throughout the course of the reconstruction of the two buildings. In the case of facadism, total colony counts were found to be 2–4 times higher indoors than outdoors, even at the initial stage of the works. High concentrations of Aspergillus and Penicillium spp. were detected. During the less intensive reconstruction, the total colony count in the indoor air samples was initially lower at almost every sampling point than at the outdoor levels. With regard to fungi, Penicillium species were initially present at lower conidia concentrations, followed by Aspergillus species over time. In both buildings, elevated concentrations of airborne fungi were detected during the main reconstruction period. The fungal genera found in the indoor air were also detected on surfaces and in dust samples. Outdoor air samples collected from the vicinity of the buildings revealed elevated fungal counts at multiple sampling points, particularly in the case of facadism. Disinfection with dry fogging was implemented twice throughout the entire interior of the buildings. Following the first disinfection process, there was no notable decrease in colony-forming unit (CFU) counts in either building. However, the second disinfection resulted in a reduction in microbial concentration in the air. Our study confirms that the renovation of historical buildings can result in an elevated prevalence of fungal bioaerosols, which can be harmful to occupants. While the impact of the reconstruction remained within the range of urban background variability at distant (>1 km) locations, it caused local microbial contamination, often exceeding the detection limit in near-site samples.

Numerous studies have shown that metagenomics has opened a dimension in reading the contents of archaeological remains as time capsules. Corn mummies are ritual objects from ancient Egypt, created by forming human-shaped figures from cereal grains grown in a mixture of water and earth. The aim of our study was to determine whether ancient DNA could be preserved in the mummy, and if so, which organisms it might have originated from. To find answers, we performed metagenomic analyses on samples taken from a corn mummy dating to the second half of the third century BC. Alongside a number of clearly modern contaminants, we identified organisms that cannot be excluded as being of historical origin. Besides considerable amounts of bacterial sequences belonging to the genus Bacillus, Mesobacillus, Metabacillus, Neobacillus, Niallia, Peribacillus and Paenibacillus , we also found traces of plants, animals, and humans. Sequences assigned to the genus Triticum showed the highest similarity to ancient T. turgidum ssp. dicoccum specimens from Egypt and the southern Levant. The fragments identified as of Lepidopteran origin showed the greatest similarity to Sphingidae genomes. Analysis of the human-derived sequences revealed L3 (mtDNA), E, and J (Y chromosome) haplotypes, which are common lineages in Africa today.

A red mud suspension of ~700,000 m³ was accidentally released from the alumina plant in Ajka, Hungary, on the 4th of October 2010, flooding several buildings in the nearby towns. As there is no information in the literature on the effects of red mud on indoor mold growth, we conducted studies to answer the following question: does the heavy metal content of red mud inhibit fungal colonization in flooded houses? In order to gain knowledge on fungal spectra colonizing surfaces soaked with red mud and on the ability of fungi to grow on them, swabs, tape lifts, and air samples were collected from three case study buildings. A total of 43 fungal taxa were detected. The dominant species were Penicillium spp. on plaster/brick walls, but Aspergillus series Versicolores, Cladosporium, Acremonium, and Scopulariopsis spp. were also present. The level of airborne penicillia was high in all indoor samples. Selected fungal strains were subcultured on 2% MEA with 10⁻¹ and 10⁻⁴ dilutions of red mud. The growth rate of most of the strains was not significantly reduced by red mud on the artificial media. The consequences of similar industrial flooding on indoor molds are also discussed in this paper.

According to reviews carried out by numerous studies from different geographic areas and by several scientific bodies, including the WHO

Honeydew extracted by aphids serves as nutrient for the development of sooty moulds. Hypothetically, population dynamics of aphids should therefore have an effect on the airborne levels of the spores of fungi colonizing honeydew. In this study, the effects of seven aphid taxa on Alternaria and Cladosporium spore seasons (both pathogenic and allergenic fungi) were analysed on a total of 20-year-long dataset in Kecskemét (Hungary), Leicester (UK) and Poznań (Poland). Meteorological factors strongly effected both aphid and fungal populations. In most cases, a direct effect of weather on fungal levels were found. Direct effect of two aphid species, Acyrthosiphon pisum and Euceraphis punctipennis, on the atmospheric concentration of Alternaria spores was identified in Leicester and Poznań in June and July respectively. Other aphid species had secondary, but significant effects during other time periods. This suggests aphid population data can be informative in predicting airborne concentrations of Alternaria and Cladosporium spores. Graphical abstract

Stormwater is a vital resource and dynamic driver of terrestrial ecosystem processes. However, processes controlling interactions during and shortly after storms are often poorly seen and poorly sensed when direct observations are substituted with technological ones. We discuss how human observations complement technological ones and the benefits of scientists spending more time in the storm. Human observation can reveal ephemeral storm-related phenomena such as biogeochemical hot moments, organismal responses, and sedimentary processes that can then be explored in greater resolution using sensors and virtual experiments. Storm-related phenomena trigger lasting, oversized impacts on hydrologic and biogeochemical processes, organismal traits or functions, and ecosystem services at all scales. We provide examples of phenomena in forests, across disciplines and scales, that have been overlooked in past research to inspire mindful, holistic observation of ecosystems during storms. We conclude that technological observations alone are insufficient to trace the process complexity and unpredictability of fleeting biogeochemical or ecological events without the shower thoughts produced by scientists’ human sensory and cognitive systems during storms.

Plants are often used to illustrate allergy-related medical products, services, patient information materials and news. The illustration of allergenic plants is an important tool in patient education, contributing to the prevention of pollinosis, as patients can recognize plants and avoid pollen exposure. In this study, it is aimed to evaluate the pictorial content of allergy-related websites depicting plants. A total of 562 different photographs depicting plants were collected using image search, identified and categorized according to their potential allergenicity. Of the total 124 plant taxa, 25% of plants were identified to the genus level and a further 68% were identified to the species level. Plants with low allergenicity were found in 85.4% of the pictures, while plants of high allergenicity were shown in only 4.5% of the pictorial information. Brassica napus was the most frequent species identified (8.9% of the overall identified plants), while blooming Prunoidae, Chrysanthemum spp. and Taraxacum officinale were also common. Considering both allergological and design aspects, some plant species have been proposed for more professional and responsible advertising. The internet has the potential to provide visual support for patient education in allergenic plants, but emphasis must be put on the transmission of the correct visual message.