Gopal Nayak

Bones, heart, liver, lungs, and brain disorders are the major concern of human overall health across the globe. The World Health Organization (WHO) estimates, in 2016

Ascorbic acid (vitamin C) acts as an antioxidant and plays a vital role in maintaining good health. The study was designed with the aim to determine the influence of the Trivedi Effect^® on the various properties of ascorbic acid by using the modern analytical techniques. The study sample was divided into two parts. The first part was considered as control (no treatment), while the second part was termed as the Biofield Energy Treated sample and it was treated with the Trivedi Effect^®-Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Gopal Nayak. The PXRD peak intensities and the crystallite sizes were significantly altered ranging from -67.75% to 1059.21% and -29.41% to 271.10% respectively, whereas the average crystallite size was significantly increased by 50.67% in the treated sample compared to the control sample. The particle size analysis of the treated ascorbic acid was reduced by 8.23% (d₁₀), 22.07% (d₅₀), 11.64% (d₉₀), and 15.81% {D(4,3)} and the surface area was increased by 15.38% as compared to the untreated sample. The weight loss was 20.57%; however, the residue amount was significantly increased by 573.99% in the treated sample compared to the control sample. The maximum thermal degradation temperatures were increased by 0.58% and 4.93%, respectively, of the treated sample compared to the control sample. The decomposition temperature was decreased by 4.17%; however, the ΔH_fusion and ΔH_{decomposition} were increased by 9.13% and 110.60%, respectively of the treated sample compared to the control sample. The Consciousness Energy Healing Treatment showed the possible formation of a new polymorph of ascorbic acid that may show better solubility, dissolution, and bioavailability, as well as more thermally stable compared to the control sample. Hence, it might be advantageous to use the Biofield Energy Treated ascorbic acid in various nutraceutical/pharmaceutical formulations to improve their efficacy. Keywords : Ascorbic acid, Consciousness Energy Healing Treatment, The Trivedi Effect^®, Complementary and Alternative Medicine, PXRD, Particle size, TGA/DTG

Sulfamethoxazole is the sulfonamide class of antibiotic that acts as a bacteriostatic agent against various bacteria. The current study was aimed to analyze the impact of the Consciousness Energy Healing Treatment (the Trivedi Effect^®) on various properties of sulfamethoxazole by using different modern analytical techniques. For this, the sample was first divided into two parts, followed by considering one part as a control sample (no treatment was given). The second part was named as the Biofield Energy Treated sample that was remotely given the Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Gopal Nayak. The particle size values were reduced by 14.35%(d₁₀), 8.93%(d₅₀), 7.84%(d₉₀), and 9.24%{D(4,3)}; therefore, the specific surface area was increased by 9.68% in the treated sample compared to the control sample. The PXRD peak intensities and crystallite sizes were significantly altered ranging from -68.71% to 40.38% and -31.58% to 169.60%, respectively; however, the average crystallite size was significantly increased by 9.49% in the treated sample compared to the control sample. The residue weight and maximum thermal degradation temperature were increased by 2.12% and 3.25%, respectively in the treated sample compared with the control sample. The decomposition temperature, latent heat of fusion, and latent heat of decomposition were significantly increased by 11.44%, 48.27%, and 22.59%, respectively in the treated sample compared to the control sample. Thus, the Trivedi Effect^®-Consciousness Energy Healing Treated sulfamethoxazole might have formed a new polymorph with reduced particle size and improved surface area and thermal stability. Hence, the use of the treated sulfamethoxazole might be more beneficial in terms of improved solubility, absorption, bioavailability, stability and also for designing the more efficacious pharmaceutical formulations for the treatment and prevention of various bacterial diseases, i.e., ear infections, urinary tract infections, shigellosis, traveler's diarrhea, bronchitis, and pneumocystis-type of pneumonia, etc. Keywords: Complementary and alternative medicine; Consciousness energy healing treatment; DSC; Particle size; PXRD; Sulfamethoxazole; The Trivedi Effect^®

Magnesium (Mg) is abundantly present in the human body, which functions for many metabolic activities, normal functioning of cells, muscles, heart, bones, etc.

The aim of the present study was to determine the impact of Biofield Energy Treated test formulation using six differentcell-lines. The test formulation/item (TI) and cell media (Med) was divided into two parts; one part was untreated (UT) and other part received Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Janice Patricia Kinney, USA and labeled as Biofield Energy Treated (BT) test item (TI)/media. Based on cell viability assay, test formulation was found as safe at tested concentrations. Cytoprotective activity of test formulation showed a significant restoration of cell viability by 60.6% (10 µg/mL), 67.5% (63.75 µg/mL), and 117.5% (63.75 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, BT-Med + BT-TI, respectively compared to untreated in human cardiac fibroblasts cells (HCF) cells. Moreover, restoration of cell viability was improved by 64% and 127.3% in UT-Med + BT-TI and BT-Med + UT-TI, respectively at 1 µg/mL compared to untreated in human liver cancer (HepG2) cells. Cellular restoration in A549 cells was improved by 314% and 112.3% at 1 µg/mL in BT-Med + UT-TI and BT-Med + BT-TI, respectively than untreated. ALP activity in Ishikawa cells was significantly increased by 175.5%, 547.2%, and 220.8% in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively at 0.1 µg/mL as compared to untreated. Additionally, in MG-63 cells showed increased ALP activity by 76.9%, 78.4%, and 79% in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively at 50 µg/mL compared to untreated. The percent cellular protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 60.6% (10 µg/mL), 67.5% (63.75 µg/mL), and 117.5% (63.75 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively as compared to untreated. An improved HepG2 cells protection (represents decreased ALT activity) by 115.1% (1 µg/mL), 42.5% (25.5 µg/mL), and 60.8% (10 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, BT-Med + BT-TI, respectively as compared to untreated. Percentage cellular protection of A549 (lungs) cells (represents increased of SOD activity) was significantly increased by 191.1% and 81.4% at 0.1 µg/mL in UT-Med + BT-TI and BT-Med + BT-TI, respectively as compared to untreated. Serotonin level was significantly increased by 31.8% (10 µg/mL) and 56.9% (25.5 µg/mL) in UT-Med + BT-TI and BT-Med + BT-TI, respectively compared to untreated in human neuroblastoma cells (SH-SY5Y). Relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 304.3% (0.01 µg/mL), 128.4% (0.1 µg/mL), and 240% (0.1 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively compared to untreated in MG-63 cells. Thus, Biofield Energy Treated test formulation (The Trivedi Effect®) significantly improved organ specific functional biomarkers and would be useful for multiple organs health related to coronary artery disease, arrhythmias, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, asthma, chronic bronchitis, cystic fibrosis, osteoporosis, etc.

Ascorbic acid is a water-soluble vitamin that mainly acts as an antioxidant and helps in controlling and maintaining various body functions.