Floris Falke
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Floris Falke has a total of 12 co-authors. Here are the top co-authors by number of shared publications:
- Andreas Schmid
Department of Solar Materials Biotechnology, 1Helmholtz Centre for Environmental Research - UFZ, Permoser Str 15, Leipzig, Germany, 04318
1 shared publication - Cecilia Pederzolli
Fondazione Bruno Kessler, Center for Sensors & Devices, Via Sommarive 18, Povo, 38123 Trento, Italy
1 shared publication - Christian Dusny
Microbial Single Cell Analysis, Department Solar Materials, Helmholtz-Centre for Environmental Research - UFZ Leipzig, Permoserstr. 15 04318 Leipzig, Germany
1 shared publication - Erik Schreuder
LioniX B.V., Enschede 7522, The Netherlands
1 shared publication - Katrin Rosenthal
School of Science Constructor University Bremen Germany
1 shared publication - Laura Pasquardini
LaBSSAH, Fondazione Bruno Kessler, Via Sommarive 18, 38123 Povo, Italy
1 shared publication
Latest Publications
- Open Access
- 6 January 2016
- Published by MDPI AG in Biosensors
- Vol. 6 (1) , 1
Asymmetric Mach–Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection
In this work, we present a study of Aflatoxin M1 detection by photonic biosensors based on Si3N4 Asymmetric Mach–Zehnder Interferometer (aMZI) functionalized with antibodies fragments (Fab′). We measured a best volumetric sensitivity of 104 rad/RIU, leading to a Limit of Detection below 5 × 10−7 RIU. On sensors functionalized with Fab′, we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.
- 6 January 2016
- Published by MDPI AG in Biosensors
- Vol. 6 (1) , 1
Open AccessAsymmetric Mach–Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection
In this work, we present a study of Aflatoxin M1 detection by photonic biosensors based on Si3N4 Asymmetric Mach–Zehnder Interferometer (aMZI) functionalized with antibodies fragments (Fab′). We measured a best volumetric sensitivity of 104 rad/RIU, leading to a Limit of Detection below 5 × 10−7 RIU. On sensors functionalized with Fab′, we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.
- Open Access
- 30 November 2015
- Published by MDPI AG in Micromachines
- Vol. 6 (12) , 1836-1855
An Inert Continuous Microreactor for the Isolation and Analysis of a Single Microbial Cell
Studying biological phenomena of individual cells is enabled by matching the scales of microbes and cultivation devices. We present a versatile, chemically inert microfluidic lab-on-a-chip (LOC) device for biological and chemical analyses of isolated microorganisms. It is based on the Envirostat concept and guarantees constant environmental conditions. A new manufacturing process for direct fusion bonding chips with functional microelectrodes for selective and gentle cell manipulation via negative dielectrophoresis (nDEP) was generated. The resulting LOC system offered a defined surface chemistry and exceptional operational stability, maintaining its structural integrity even after harsh chemical treatment. The microelectrode structures remained fully functional after thermal bonding and were proven to be efficient for single-cell trapping via nDEP. The microfluidic network consisted solely of glass, which led to enhanced chip reusability and minimized interaction of the material with chemical and biological compounds. We validated the LOC for single-cell studies with the amino acid secreting bacterium Corynebacterium glutamicum. Intracellular l-lysine production dynamics of individual bacteria were monitored based on a genetically encoded fluorescent nanosensor. The results demonstrate the applicability of the presented LOC for pioneering chemical and biological studies, where robustness and chemically inert surfaces are crucial parameters for approaching fundamental biological questions at a single-cell level.
- 30 November 2015
- Published by MDPI AG in Micromachines
- Vol. 6 (12) , 1836-1855
Open AccessAn Inert Continuous Microreactor for the Isolation and Analysis of a Single Microbial Cell
Studying biological phenomena of individual cells is enabled by matching the scales of microbes and cultivation devices. We present a versatile, chemically inert microfluidic lab-on-a-chip (LOC) device for biological and chemical analyses of isolated microorganisms. It is based on the Envirostat concept and guarantees constant environmental conditions. A new manufacturing process for direct fusion bonding chips with functional microelectrodes for selective and gentle cell manipulation via negative dielectrophoresis (nDEP) was generated. The resulting LOC system offered a defined surface chemistry and exceptional operational stability, maintaining its structural integrity even after harsh chemical treatment. The microelectrode structures remained fully functional after thermal bonding and were proven to be efficient for single-cell trapping via nDEP. The microfluidic network consisted solely of glass, which led to enhanced chip reusability and minimized interaction of the material with chemical and biological compounds. We validated the LOC for single-cell studies with the amino acid secreting bacterium Corynebacterium glutamicum. Intracellular l-lysine production dynamics of individual bacteria were monitored based on a genetically encoded fluorescent nanosensor. The results demonstrate the applicability of the presented LOC for pioneering chemical and biological studies, where robustness and chemically inert surfaces are crucial parameters for approaching fundamental biological questions at a single-cell level.