Elena Brunori

Urban vineyards are expanding worldwide, driven by cultural and environmental motivations and their potential contribution to sustainable food systems. This study assessed grapevine (Vitis vinifera L.) responses to urban atmospheric conditions and explored traits relevant to cultivar selection and particulate matter (PM) interception. Research was conducted during the 2025 growing season in a highly biodiverse urban vineyard comprising 170 cultivars at the Botanical Garden of Rome (Italy), a city where grapevine has been documented since antiquity. Digital phenotyping was used to screen 54 cultivars based on leaf micromorphological traits, followed by detailed assessment of five selected cultivars. Measurements also included leaf anatomical traits, chlorophyll content, spectral reflectance-derived indices related to vigor and water status, and PM interception by scanning electron microscopy. Cultivars differed in micromorphological traits and ecophysiological proxies, suggesting different responses to urban environmental conditions. Total leaf-surface PM accumulation after an approximately 20-day accumulation period following the last intense rainfall event (>10 mm h⁻¹) ranged from 52,024 particles mm⁻² in cv. Barbera to 77,462 particles mm⁻² in cv. Maturano, values broadly comparable to those reported for some urban forest species. Deposition on the adaxial surface was more than twice that measured on the abaxial surface. These findings highlight the potential of digital phenotyping to integrate morphological and physiological traits, supporting plant material selection for urban viticulture, PM interception, and, more broadly, for urban landscape design.

Cork oak (Quercus suber L.) forests play a crucial role in the Mediterranean region, providing essential ecological, social, and economic services. Increasing pressure from wildfires, pests, diseases, and climate change has led to a progressive decline of these ecosystems, making the development of innovative post-stripping management strategies urgent. This study evaluates the effectiveness of kaolin application on cork oak trees immediately after cork removal in a mixed forest in Sant Celoni (Barcelona, Spain). Short- and long-term physiological responses were assessed through stomatal conductance and chlorophyll fluorescence (OJIP test), while sap flux density (J_s) was continuously monitored over a four-month period (July–October 2023) using IoT-based TreeTalker® Cyber (Nature 4.0 s.r.l., Viterbo, Italy). Proximal vegetation indices (Normalized Difference Vegetation Index, NDVI; and Normalized Difference Red Edge, NDRE) were also evaluated but showed no significant differences among treatments (p > 0.05). Kaolin-treated trees (K) maintained significantly higher photosynthetic performance and stem water transport capacity compared to untreated stripped trees (nK), with effects persisting up to 140 days after stripping. These findings support kaolin application as a viable and low-cost tool for mitigating post-stripping physiological stress in cork oak forest management. Further research across multiple sites and consecutive harvesting cycles is recommended to fully assess its long-term implications for tree vitality and cork productivity.

The need to identify strategies to mitigate extreme abiotic conditions has become urgent, as these factors directly influence the yield and quality of viticultural production. This study aims to evaluate the physiological and productive effects of applying a biological leaf corroborant - Basalt Flour® (FB) on autochthonous grapevine varieties and international ones under different sensitive areas for climate conditions: a peri-urban Rome (Velletri) and a minor Mediterranean island (Ventotene). During three consecutive seasons (2023-2025) micro-environmental parameters of the vineyard were monitored, and historical climate data were analysed to assess the climatic vulnerability. Peri-urban vineyards are integrated within the urban fabric, making them vulnerable and more exposed to likely, intense, and frequent climatic fluctuations. On the other hand, vineyards of minor Mediterranean islands are affected by abiotic stressors (climate, soil salinity, water deficit) combined with socio-economic processes such as land abandonment and require a multifaceted approach combining climate resilience, land management, and community engagement, to preserve cultural heritage, biodiversity and local economies. The health status of the vine was monitored using non-destructive tools capable of measuring leaf chlorophyll content, chlorophyll fluorescence efficiency, and vegetative indices to evaluate the plant's water and nutritional status. The results highlight a genotype-dependent response. Overall, foliar application of FB delays leaf senescence, reduces abiotic stress, improves gas exchange, enhances the photosynthetic performance of the vine, and improves the hydraulic status of the canopy. The effect of FB is more pronounced in seasons with more limiting climatic conditions. In conclusion, foliar application of FB proves to be a promising strategy to enhance the resilience of the vine to climatic adversities, thus ensuring the sustainability of viticulture under semi-arid conditions. These findings offer valuable insights for future agronomic practices aimed at maintaining quality and productivity in vulnerable viticultural contexts such as urban, peri-urban and coastal ones.

Water scarcity can negatively affect crop yield, posing a significant threat to global food security, such as drought. Plant growth-promoting rhizobacteria (PGPR), either as single strains or synthetic communities (SynComs), has shown promise in alleviating drought stress in various plant species. In this study, we examined the effects of water limitation on Salvia officinalis and the potential of a SynCom composed of five phosphate-solubilizing, auxin-producing, and/or nitrogen-fixing Gram-negative bacteria to enhance plant growth and drought tolerance. Plant growth, morphology, physiology, and leaf metabolomic profiles were assessed using a combination of physiological measurements and LC-MS untargeted metabolomics. Mild water stress induced a conservative water-use strategy in S. officinalis, characterized by increased root-to-shoot ratio and altered leaf morphology, without compromising photosynthetic performance. SynCom inoculation under well-watered conditions elicited drought-like responses, including transient reductions in stomatal conductance. Leaf metabolomic analysis revealed that inoculation influenced the abundance of several metabolites, including biogenic amines and dipeptides, under both irrigation regimes. Notably, drought stress and SynCom inoculation increased histamine and α-ketoglutaric acid levels, highlighting potential impacts on food quality. Under reduced irrigation, inoculation further modulated leaf morphology and biomass allocation, promoting thicker leaves and increased root biomass allocation. These results demonstrate the ability of the SynCom to modulate plant physiology and metabolism in response to both optimal and reduced irrigation, potentially enhancing drought resilience without directly improving growth. The study also highlights the complex interactions among microbial inoculation, plant stress responses, and leaf metabolite profiles, emphasizing the importance of considering the effects on the production of bioactive compounds when developing microbial inoculants for edible plants.

Landslides affecting soil layers up to 1–2 m deep pose a significant hazard in mountainous and hilly regions, particularly in the Mediterranean, where intense precipitation is increasing. Identifying landslide-prone areas is crucial for risk assessment and mitigation, as landslides can severely impact land surfaces, infrastructure, and inhabited areas. Forest cover and management play a fundamental role in stabilizing soil and reducing landslide susceptibility. This study focuses on landslide forecasting models, which integrate geological, climatic, and topographic data to predict landslide probability and severity. Specifically, we compare the predictive accuracy of the 4SLIDE model with the established SHALSTAB model in a forested mountain catchment within Sila National Park, Southern Italy, using GIS-based analysis. The results demonstrate that both models effectively identify high-risk areas, with ROC analysis confirming the superior predictive capability of the 4SLIDE model. Our findings underscore the critical importance of early landslide identification, supporting timely interventions and the implementation of forest engineering and Civil Protection measures to mitigate the impact of landslides on communities and infrastructure.

Future climate conditions may jeopardize the suitability of traditional grape-growing areas in the Mediterranean. However, precise vineyard management is a crucial component of adaptation strategies aimed at optimizing resource efficiency, which is essential for sustainable farming practices. A fine-scale characterization, based on the spatial variability of soil’s physical–chemical and hydrological traits combined with temporal variability of vine canopy temperature extracted from UAV thermal images has been adopted in a rainfed vineyard of central Italy, for better understanding the impact of soil and climate abiotic factors in the vineyard for planning precision adaptation strategies encouraging sustainable resource use. This study identifies significant soil heterogeneity within the tested vineyard, affecting water retention, nutrient availability, and vine water stress. We combined ground-based measurements with remote sensing-enhanced data spatialization and helped to advocate for site-specific management techniques as short- and long-term strategies (such as canopy management, deficit irrigation, and compost application) to counter climate emergencies, restore soil health, and preserve vine function and economic yields.

The soil microbiota is a key component of agroecosystems, and understanding its traits is crucial for effective agronomic management. Among beneficial microorganisms, arbuscular mycorrhizal fungi (AMFs) are mutually associated with grapevine (Vitis vinifera L.), enhancing the ability of this cropping system to adapt to soil conditions and bolstering its resistance and resilience against abiotic stresses, particularly drought, by promoting root growth and enhancing the roots’ absorption surface. The objective of this on-field study was to determine AMF species richness and diversity along with their relation to soil chemical, physical, and biological characteristics in two adjacent organic vineyards in Central Italy. The two tested vineyards of the autochthonous cv. Aleatico differed by the presence of grafted (Vitis berlandieri × V. riparia rootstock; AL-420) or own-rooted (ungrafted V. vinifera L.; AL-ORV) vines. To this aim, soil and root samples were collected and geo-referenced. Analysis of the AMF species colonizing roots of both AL-ORV and AL-420 revealed the presence of four species: Scutellospora alterata, Paraglomus laccatum, Acaulospora laevis, and A. baetica, with S. alterata being the most frequent. Mycorrhization parameters were higher in the roots of grafted plants compared to ungrafted ones. A high beta-glucosidase (BG):N-acetylglusosaminidase (NAG) ratio in two tested vineyards indicated that microbes utilized more cellulose than chitin and peptidoglycan as dominant C resources. A negative correlation between mycorrhization rate (MyCP) and BG was observed, likely because AMFs form mutualistic relationships with plants, depending on the host plant for carbon. Results revealed a positive correlation between the degree of mycorrhizal association and the species involved, with the presence of copper and nickel among metals. Negative correlations were found concerning soil clay content along with beta-glucosidase. In conclusion, the grapevine root system was characterized by a differential symbiotic relationship with AMF species, whose development is influenced by the root genotype, soil texture, and biochemistry. Specifically, the increased frequency of AMFs in relation to copper content strengthens the evidence of their role in maintaining a vine’s production capacity in the event of soil contamination by this element.

Agrobiodiversity represents a system of biological organisms that contribute to agri-food production. In a context marked by a significant loss of food-relevant species and a reduction in their genetic diversity, the adoption of strategies to preserve and enhance the diversity of genetic resources that support and complement agricultural production has become a global challenge. Many sustainable development strategies outlined in recent years directly and indirectly attribute a crucial role to agrobiodiversity in meeting food needs, ensuring food system security, promoting food justice, and enhancing well-being in modern living environments. This contribution aims to analyze the process of knowledge and awareness that has led many cities to plan their urban development by investing in the agricultural matrix and to address the design of open spaces with agricultural biodiversity.

Soil CO₂ efflux is a pivotal component of agro-ecosystem C budgets. It is considered a proxy indicator of biological activity and a descriptor of soil quality that is strongly linked to agricultural soil management. We investigated the effects of soil fertilization practices (organo-mineral (OMN) versus chemical (C)) on soil under-vine CO₂ efflux (TSR) in an Italian rainfed vineyard (cv Chardonnay). The TSR was measured using the chamber technique as follows: a close multi-chamber system (prototype) was placed under a vine. Data (CO₂, temperature, and moisture) were acquired hourly during two consecutive years (2021 and 2022) from flowering to berry ripening. Physical–hydrological soil parameters were determined, and the seasonal trends of the TSR, soil temperature, and soil moisture were assessed. The TSR measurements fluctuated for the 2021 season, ranging from 1.03 to 1.97 µmol CO₂∙m⁻²∙s⁻¹ for the C treatment, while for the OMN treatment, the TSR measurements ranged from 1.24 to 1.71 µmol CO₂∙m⁻²∙s⁻¹. Extreme weather conditions (2022) highlighted the differences between the two agronomical practices, and a decoupling was found between the TSR and the soil water content, with the TSR being controlled primarily by the soil temperature. At the daily scale, the findings showed that the TSR reached its minimum in the early morning hours (5:00–8:00). The results promote organic–mineral nutrition as an improved practice for soil carbon storage (restoration of the organic fraction) by reducing the TSR, permitting the preservation of soil quality and stabilizing the hydrological traits by preserving the biotic activities.

Plant–soil biota represent a unique living system crucial for improving crops’ adaptation to climate change. In vineyards, plant–soil relations are mediated by rootstock–scion interaction, with grafted vines being the main plant material employed in vineyard planting. The interaction between two deeply different biological systems such as Vitis vinifera sativa and the American Vitis species may modify vines’ adaptation to abiotic stress. The aim of this study was to investigate the physiological response (chlorophyll content—CHL; stomatal conductance—gs) of grafted and ungrafted vines and assess the involvement of Arbuscular Mycorrhizal Fungi (AMF) in this response. In two vineyards located in Central Italy, the presence of AMF was assessed in the roots of grafted and ungrafted cv ‘Aleatico’ vines. The morphological traits of AMF and root mycorrhizal colonization differed in the grafted and ungrafted vines. Under limited climate conditions, ungrafted vines showed better leaf resilience traits (high CHL and gs values) and larger AMF storage organs (vesicles). On the other hand, the grafted ones—which are more sensitive to climate conditions (lower gs and CHL)—involved AMF colonization strategies (greater abundance of arbuscoles and mycorrhizal colonization potential) linked to the improved uptake and transport of water from the bulk soil to the vine. Taken together, these findings highlight different mycorrhizal colonization strategies and storage behaviors in grafted and ungrafted vineyards and with respect to different physical and chemical soil traits.