Patrizia Fava

Acrylamide is a key Maillard-derived contaminant in biscuits, yet mitigation strategies are often expected to compromise quality. We evaluated citric, tartaric, gallic, and L-ascorbic acids as sodium bicarbonate neutralizing agents in biscuits produced from semi-whole wheat flour with high free asparagine, bran components, and stabilized wheat germ oil. Acrylamide was determined by LC-MS/MS and interpreted with color, moisture-related parameters, mechanical behavior, antioxidant capacity, and sensory data. All acid treatments significantly lowered acrylamide relative to the control (−58.5% to −78.8%), with final concentrations well below EU benchmark levels. L-ascorbic and tartaric acids gave the strongest mitigation, whereas gallic acid, applied at reduced level to avoid bitterness, was less effective, showing that antioxidant capacity alone is insufficient. Browning metrics did not reliably predict acrylamide formation. Selected organic acids therefore provide an effective reformulation strategy with limited impact on product quality.

Tenebrio molitor (mealworm) has emerged as a sustainable protein source for baked goods, but its effects on texture, digestibility, safety (acrylamide formation) and sensory quality over time in sweet bakery products remain insufficiently explored. This study aimed to develop a novel, protein-enriched biscuit formulation by partially replacing wheat flour with T. molitor powder (5% (w/w) and 10% (w/w)) and incorporating freeze-dried sour cherry powder to enhance sensory appeal and moisture retention. A multidisciplinary assessment was conducted, including proximate analysis, acrylamide determination, in vitro digestion to evaluate protein bioaccessibility, textural parameters and an accelerated shelf-life study (through sensory analysis). Results demonstrated that T. molitor incorporation significantly increased protein content (28.57 and 51.95% in biscuits with 5 (w/w) and 10% (w/w) T. molitor powder, respectively), as well as Protein/Carbohydrate ratio and the Protein/Fat ratio, while reducing biscuit breaking resistance and, in turn, improving biscuit friability. Acrylamide levels remained below regulatory limits (≤30 μ g/kg), while partial wheat flour substitution with T. molitor powder improved protein digestibility. Notably, biscuits with 10% (w/w) T. molitor showed reduced hardness and maintained good sensory stability during storage. Overall, these results support the technological feasibility and industrial potential of T. molitor as a sustainable protein ingredient in bakery products.

Background: The pandemic and lockdown caused a slowdown or halt in many work activities across sectors, including academic research, which had to adapt lab procedures to lockdown restrictions. This study aimed to assess an innovative approach to sensory analysis that aligned with the pandemic’s constraints and could enhance traditional methods even in normal conditions. Methods: Remote training of judges was conducted to test the method’s effectiveness. Sensory evaluation of sous-vide chicken breast fillets was conducted at different temperatures (60, 70, 80 °C) and time combinations (60, 90, 120, 150 min), compared to a control (boiled at 100 °C for 60 min). Judges tasted 6 out of 13 randomized samples, recording intensities on a cloud-based sensory card. Results: Judges demonstrated good repeatability and panel homogeneity (RSD ≤ 30%). Significant differences (p < 0.05) in olfactory and flavor characteristics were noted among samples. Higher-temperature samples had stronger boiled meat and chicken flavors, and sous-vide samples showed greater juiciness, especially LT2 and LT3. Conclusions: The remote home-tasting approach proved effective in distinguishing key differences in meat characteristics based on cooking conditions. This method’s reliability and adaptability make it a promising alternative to lab-based sensory evaluation, ensuring research continuity in restrictive conditions and broadening potential for decentralized studies.

Paper is one of the most promising materials for food packaging and wrapping due to its low environmental impact, but surface treatments are often needed to improve its performance, e.g., the resistance to fats and oils. In this context, this research is focused on the formulation of a new paper bio-coating. Paper was coated with liquids containing poly(hexano-6-lactone) (PCL), glycerol and variable percentages of starch (5–10% w/w PCL dry weight), agar-agar (0–1.5% w/w PCL dry weight), and polyethylene glycol (PEG) (5% or 15% w/w PCL dry weight) to improve coating uniformity and diffusion. A design of experiments approach was implemented to find statistically reliable results in terms of the best coating formulation. Coated paper was characterized through mechanical and physical properties. Results showed that agar content (1.5% w/w PCL dry weight) has a beneficial effect on increasing the resistance to oil. Furthermore, the best coating composition has been calculated, and it is 10% w/w PCL dry weight of starch, 1.5% w/w PCL dry weight of agar, and 15% w/w PCL dry weight of PEG.

Paper is one of the most promising materials for food packaging and wrapping due to its low environmental impact, but surface treatments are often needed to improve some performance, e.g. the resistance to fats and oils. In this context, this research is focused on the formulation of a new paper bio-coating. Paper was coated with liquids containing Poly(hexano-6-lactone) (PCL), Glycerol and variable percentages of starch (5 - 10% w/w PCL dry weight), agar agar (0 - 1.5% w/w PCL dry weight) and PEG (5% or 15% w/w PCL dry weight) to improve coating uniformity and diffusion. A Design of Experiments approach was implemented to find statistically reliable results in terms of best coating formulation. Coated paper was characterized through mechanical and physical properties. Results showed that agar content (1.5% w/w PCL dry weight) has a beneficial effect on increasing the resistance to oil. Furthermore, the best coating composition has been calculated, and it is: 10% w/w PCL dry weight of Starch, 1.5% w/w PCL dry weight of agar and 15% w/w PCL dry weight of PEG.

The present study assessed the effectiveness of high pressure processing (HPP) for the quality maintenance of pitted sour cherries, with special regard to microbial stabilization and the maintenance of color and of chemical–nutritional properties. The HPP treatment (600 MPa for 3 min at 4 °C) was effective at minimizing the initial microbial load, which remained at negligible levels throughout 5 months of refrigerated storage. The color and total phytochemical content of sour cherries were not influenced by the HPP treatment and were maintained at levels comparable with the fresh product for 3 months of refrigerated storage. For longer storage periods, the typical red color decreased, in agreement with the content of total anthocyanins, which showed a significant decrease (up to 65% after 5 months). The antioxidant activity, measured by the ABTS and DPPH assays, was not affected by the HPP treatment, but slightly reduced during refrigerated storage. The study suggests that HPP may be exploited to extend the shelf life, while maintaining the fresh-like features of sour cherries, thus offering an alternative option to current preservation techniques (based on freezing or heating) commonly applied to this product.

Acrylamide in biscuits represents a major concern. This research work was aimed at modifying the current formulation of biscuits to reduce the acrylamide content while maintaining the chemical, physical, and sensory characteristics of the original product. A strategy based on the FoodDrinkEurope Acrylamide Toolbox was adopted. The content of the leavening agent ammonium bicarbonate, the baking temperature program, and the time duration of steam released during the baking process were the three factors evaluated through a factorial design of experiment. The partial replacement of ammonium bicarbonate (from 9.0 g to 1.5 g per 500 g of flour) with sodium bicarbonate (from 4.5 g to 12.48 g), lowering of the temperature in the central phase of the baking process (from 170 °C to 150 °C), and the release of steam for 3 min resulted in an 87.2% reduction in acrylamide concentration compared to biscuits of reference. CIELab color indices and a_w were the parameters that showed the most significant correlation with acrylamide concentration in biscuits and could, therefore, become markers to predict the acrylamide content along production lines for an instant evaluation.

Coffee capsules have become one of the most used methods to have a coffee in the last few years. In this work, coffee was prepared using a professional espresso coffee machine. We investigated the volatilome of four different polypropylene coffee capsule typologies (Biologico, Dolce, Deciso, Guatemala) with and without capsules in order to reveal the possible differences in the VOCs spectra. The volatilome of each one was singularly studied through an analysis by gas chromatography and mass spectrometry (GC–MS), checking the abundance of different VOCs in coffee extracted with and without a capsule protection and compared to its related sample. Furthermore, ANOVA and Tukey tests were applied to statistically identify and individuate the possible differences. As a result, it was found that coffee capsules, offer advantages of protecting coffee from oxidation or rancidity and, consequently extended shelf life as well as did not cause a reduction of volatile compounds intensity. Therefore, it is possible to conclude that the aroma of polypropylene coffee capsule extraction is not damaged compared to a traditional espresso.

Surface hydrophobicity and grease resistance of paper may be achieved by the application of coatings usually derived from fossil-oil resources. However, poor recyclability and environmental concerns on generated waste has increased interest in the study of alternative paper coatings. This work focuses on the study of the performances offered by two different biopolymers, poly(3-hydroxybutyrate-co-3hydroxyvalerate) (PHBV) and polycaprolactone (PCL), also assessing the effect of a plasticizer (PEG) when used as paper coatings. The coated samples were characterized for the structural (by scanning electron microscopy, SEM), diffusive (water vapor and grease barrier properties), and surface properties (affinity for water and oil, by contact angle measurements). Samples of polyethylene-coated and fluorinated paper were used as commercial reference. WVTR of coated samples generally decreased and PHBV and PCL coatings with PEG at 20% showed interesting low wettability, as inferred from the water contact angles. Samples coated with PCL also showed increased grease resistance in comparison with plain paper. This work, within the limits of its lab-scale, offers interesting insights for future research lines toward the development of cellulose-based food contact materials that are fully recyclable and compostable.