Hossein Davoudi

Southwest and Central Asia were pivotal regions for the domestication and dispersal of domestic herbivores. Here we investigate long-term variation in bony labyrinth morphology of domestic bovids (sheep, goats and cattle) across the last 10 000 years. We focused on the osseous cavity of the inner ear, located within the petrous bone. We analysed semicircular canal (SC) size and shape using micro-computed tomography and geometric morphometrics (GMM) on 78 modern reference specimens and 234 archaeological petrous bones from 51 sites spanning the Iranian Plateau, Central Asia, the Caucasus and the Indus Valley. Bony labyrinth morphometrics yielded a strong taxonomic signal, enabling species-level identification of 228 out of 234 specimens and revealing greater diversity than anticipated. Comparative analyses further documented contrasting, nonlinear diachronic trajectories in SC size and shape among sheep, goats and cattle, reflecting species-specific responses to domestication and long-term herd management. Together, these results demonstrate that bony labyrinth GMM provides a robust, non-destructive framework for taxonomic identification and evolutionary inference and underscore how long-term, interdisciplinary approaches can capture the complex, nonlinear dynamics of animal domestication across the longue durée.

Animal domestication and development of pastoralism in southwest Asia revolutionized human subsistence strategies. Various centres of ruminant domestication and diffusion routes of agropastoralism have been identified. The area between the northern and central Zagros Mountains on the Iranian Plateau is a cradle for goat domestication and eastward spread of agropastoralism. However, the early exploitation of ruminant milk by pastoral communities in the Zagros remains insufficiently studied. Here we show residues of caprine dairy products that were detected from the analysis of lipid residues in pottery vessels and protein residues in human dental calculus. These results, combined with the faunal spectra and radiocarbon analyses directly on the dairy residues, show that sheep and goat dairy products were widely exploited in the Zagros from the seventh millennium BC. This pattern parallels the contemporaneous exploitation of cattle milk in Anatolia. Neolithic communities in both regions reveal similarly complex dynamics of early ruminant milk use, marking the emergence of independent yet synchronous trajectories in the diffusion of agropastoral lifeways.

The origins and prehistory of domestic sheep ( Ovis aries ) are incompletely understood; to address this, we generated data from 118 ancient genomes spanning 12,000 years sampled from across Eurasia. Genomes from Central Türkiye ~8000 BCE are genetically proximal to the domestic origins of sheep but do not fully explain the ancestry of later populations, suggesting a mosaic of wild ancestries. Genomic signatures indicate selection by ancient herders for pigmentation patterns, hornedness, and growth rate. Although the first European sheep flocks derive from Türkiye, in a notable parallel with ancient human genome discoveries, we detected a major influx of Western steppe–related ancestry in the Bronze Age.

The Douzlākh salt deposit (region: Māhneshān, Zanjān Province, Iran) is unique for its pure and crystal rock salt and was an important supplier of culinary (‘table’) salt in Achaemenid, Sassanid and Middle Islamic times. At the same time, the site was of central importance to the economic life of the rural populations in the Talkherud Basin. This article focuses on the question of which strategies were decisive for the exploitation of the salt mountain and how a potential supra-regional interest in the culinary salt was perhaps reciprocally connected with a rural hinterland. This hinterland was recently investigated in greater detail by our ongoing research. Did a resource-scape based on salt develop with specific economic and social strategies and practices around the Douzlākh? And was this development triggered by state or imperial control and demand? These questions are being pursued from a perspective utilising a variety of subjects and methods in archaeology, archaeobiology, archaeometry and geoarchaeology. In addition to a detailed on-site artefact study, several on- and off-site datasets have been collected and analysed within a multidisciplinary framework. This article synthesises the results of a major 12-year project to identify the organisational principles and daily practices within this specific salt-scape. The sensational finds of the Douzlākh salt mummies, along with the generally outstanding preservation of organic ecofacts and artefacts, allow insights into antique lifeworlds that are otherwise hard to come by. The multidisciplinary study of on- and off-site data allows far-reaching insights into interdisciplinary topics, such as the social system, supply and logistics, or the presence of non-local or non-indigenous populations.

The pronounced growth in livestock populations since the 1950s has altered the epidemiological and evolutionary trajectory of their associated pathogens. For example, Marek’s disease virus (MDV), which causes lymphoid tumors in chickens, has experienced a marked increase in virulence over the past century. Today, MDV infections kill >90% of unvaccinated birds, and controlling it costs more than US$1 billion annually. By sequencing MDV genomes derived from archeological chickens, we demonstrate that it has been circulating for at least 1000 years. We functionally tested theMeqoncogene, one of 49 viral genes positively selected in modern strains, demonstrating that ancient MDV was likely incapable of driving tumor formation. Our results demonstrate the power of ancient DNA approaches to trace the molecular basis of virulence in economically relevant pathogens.

Equids have shaped past Eurasian societies in many ways. This applies in particular to domestic horses, donkeys, and their hybrids. Key to documenting modes of exploitation and cultural trajectories in past societies is the correct taxonomic classification of tooth and bone specimens found in archaeological sites. However, close osteomorphological resemblance of wild and domestic equids and their economically valuable hybrids, that is, mules and hinnies, complicates the identification of intentionally fragmented or naturally damaged archaeological specimens. Here, we apply geometric morphometrics (GM) to mandibular teeth and metapodials, two skeletal elements commonly found in archaeological collections and known for their diagnostic properties using traditional morphometric methods. We registered a statistically representative set of 2D and 3D coordinates on mandibular teeth (P3, P4, M1, and M2) and metapodials of 92 domestic horses (Equus caballus Linnaeus, 1758), 70 domestic donkeys (Equus asinus Linnaeus, 1758), 30 hybrids, and 63 Asiatic wild asses (Equus hemionus Pallas, 1775). Taxonomic classification of these 255 specimens considered both shape and form, applying linear discriminant analysis, k‐nearest neighbors algorithm, and artificial neural networks to seven combinations of taxa. We obtained correct classifications in over 87% and 80%, respectively, of the premolars and molars and in over 93% and 89%, respectively, of the metacarpals and metatarsals. This modern dataset was then used to classify equid specimens from three archaeological sites in the Middle East already analyzed morphologically. Taking into account the past zoogeography of wild equids and the historical distribution of their domesticated descendants and hybrids, the GM approach presented in this study offers the possibility to morphologically classify archaeological equids with far greater certainty than has been the case so far.

Donkeys transformed human history as essential beasts of burden for long-distance movement, especially across semi-arid and upland environments. They remain insufficiently studied despite globally expanding and providing key support to low- to middle-income communities. To elucidate their domestication history, we constructed a comprehensive genome panel of 207 modern and 31 ancient donkeys, as well as 15 wild equids. We found a strong phylogeographic structure in modern donkeys that supports a single domestication in Africa ~5000 BCE, followed by further expansions in this continent and Eurasia and ultimately returning to Africa. We uncover a previously unknown genetic lineage in the Levant ~200 BCE, which contributed increasing ancestry toward Asia. Donkey management involved inbreeding and the production of giant bloodlines at a time when mules were essential to the Roman economy and military.