Xianying Liu
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Xianying Liu has a total of 8 co-authors. Here are the top co-authors by number of shared publications:
Jie Song
Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Jilin University, Changchun 130041, China
1 shared publicationLirong Zhang
Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun 130031, China
1 shared publicationNing Xi
Hospital Office, People's Liberation Army No. 93246, Changchun 130051, China
1 shared publicationShanshan Song
Department of Radiation, School of Public Health, Jilin University, Jilin 130031, China
1 shared publicationWenjing Huang
Jilin Province Yitong Secondary Health Vocational School, Siping 130000, China
1 shared publication- Yingjun Xie
Department of Hepatobiliary and Pancreatic Surgery, Second Hospital of Jilin University, Changchun 130041, China
1 shared publication
Latest Publications
- Open Access
- 1 January 2016
- Published by Wiley in Oxidative Medicine and Cellular Longevity
- Vol. 2016 (1) , 1204715
Partial Oxygen Pressure Affects the Expression of Prognostic Biomarkers HIF‐1 Alpha, Ki67, and CK20 in the Microenvironment of Colorectal Cancer Tissue
Hypoxia is prognostically important in colorectal cancer (CRC) therapy. Partial oxygen pressure (pO2) is an important parameter of hypoxia. The correlation between pO2 levels and expression levels of prognostic biomarkers was measured in CRC tissues. Human CRC tissues were collected and pO2 levels were measured by OxyLite. Three methods for tissue fixation were compared, including formalin, Finefix, and Finefix‐plus‐microwave. Immunohistochemistry (IHC) staining was conducted by using the avidin‐biotin complex technique for detecting the antibodies to hypoxia inducible factor‐1 (HIF‐1) alpha, cytokeratin 20 (CK20), and cell proliferation factor Ki67. The levels of pO2 were negatively associated with the size of CRC tissues. Finefix‐plus‐microwave fixation has the potential to replace formalin. Additionally, microwave treatment improved Finefix performance in tissue fixation and protein preservation. The percentage of positive cells and gray values of HIF‐1 alpha, CK20, and Ki67 were associated with CRC development (P < 0.05). The levels of pO2 were positively related with the gray values of Ki67 and negatively related with the values of HIF‐1 alpha and CK20 (P < 0.05). Thus, the levels of microenvironmental pO2 affect the expression of predictive biomarkers HIF‐1 alpha, CK20, and Ki67 in the development of CRC tissues.
- 1 January 2016
- Published by Wiley in Oxidative Medicine and Cellular Longevity
- Vol. 2016 (1) , 1204715
Open AccessPartial Oxygen Pressure Affects the Expression of Prognostic Biomarkers HIF‐1 Alpha, Ki67, and CK20 in the Microenvironment of Colorectal Cancer Tissue
Hypoxia is prognostically important in colorectal cancer (CRC) therapy. Partial oxygen pressure (pO2) is an important parameter of hypoxia. The correlation between pO2 levels and expression levels of prognostic biomarkers was measured in CRC tissues. Human CRC tissues were collected and pO2 levels were measured by OxyLite. Three methods for tissue fixation were compared, including formalin, Finefix, and Finefix‐plus‐microwave. Immunohistochemistry (IHC) staining was conducted by using the avidin‐biotin complex technique for detecting the antibodies to hypoxia inducible factor‐1 (HIF‐1) alpha, cytokeratin 20 (CK20), and cell proliferation factor Ki67. The levels of pO2 were negatively associated with the size of CRC tissues. Finefix‐plus‐microwave fixation has the potential to replace formalin. Additionally, microwave treatment improved Finefix performance in tissue fixation and protein preservation. The percentage of positive cells and gray values of HIF‐1 alpha, CK20, and Ki67 were associated with CRC development (P < 0.05). The levels of pO2 were positively related with the gray values of Ki67 and negatively related with the values of HIF‐1 alpha and CK20 (P < 0.05). Thus, the levels of microenvironmental pO2 affect the expression of predictive biomarkers HIF‐1 alpha, CK20, and Ki67 in the development of CRC tissues.