Jolanta Anna Watson

Surfaces based on both chemical and physical mechanisms of attack may yield the next generation of bactericidal and antibacterial surfaces. A simple model is presented based on surface energies explaining the physical action of attack on bacteria (deformation/rupture) by nanopillar surfaces. The analysis is subject to three important constraints which impact on the optimal nanopillar radius, spacing, and length: 1) Nanopillar radius >> the Minimum nanopillar radius which allows for cell wall conformation (so that the surface energy releases when the outer cell layer binds to the nanopillar > work required to bend the layer around the nanopillar). 2) The inter‐nanopillar spacing must be << the size of the bacterial cell (so that the cell becomes bound to several nanopillars). 3) The length of the nanopillars must be greater than the distance that the bacteria cell wall would be pulled along the nanopillar shaft.

In this study, we have investigated the micro- and nano-structuring and contaminant adhesional forces of the outer skin layer of the ground dwelling gecko— Lucasium steindachneri. The lizard's skin displayed a high density of hairs with lengths up to 4 μm which were spherically capped with a radius of curvature typically less than 30 nm. The adhesion of artificial hydrophilic (silica) and hydrophobic (C ₁₈ ) spherical particles and natural pollen grains were measured by atomic force microscopy and demonstrated extremely low values comparable to those recorded on superhydrophobic insects. The lizard scales which exhibited a three-tier hierarchical architecture demonstrated higher adhesion than the trough regions between scales. The two-tier roughness of the troughs comprising folding of the skin (wrinkling) limits the number of contacting hairs with particles of the dimensions used in our study. The gecko skin architecture on both the dorsal and trough regions demonstrates an optimized topography for minimizing solid–solid and solid–liquid particle contact area, as well as facilitating a variety of particulate removal mechanisms including water-assisted processes. These contrasting skin topographies may also be optimized for other functions such as increased structural integrity, levels of wear protection and flexibility of skin for movement and growth. While single hair adhesion is low, contributions of many thousands of individual hairs (especially on the abdominal scale surface and if deformation occurs) may potentially aid in providing additional adhesional capabilities (sticking ability) for some gecko species when interacting with environmental substrates such as rocks, foliage and even man-made structuring.

Condensation resulting in the formation of water films or droplets is an unavoidable process on the cuticle or skin of many organisms. This process generally occurs under humid conditions when the temperature drops below the dew point. In this study, we have investigated dew conditions on the skin of the gecko Lucasium steindachneri . When condensation occurs, we show that small dew drops, as opposed to a thin film, form on the lizard's scales. As the droplets grow in size and merge, they can undergo self-propulsion off the skin and in the process can be carried away a sufficient distance to freely engage with external forces. We show that factors such as gravity, wind and fog provide mechanisms to remove these small droplets off the gecko skin surface. The formation of small droplets and subsequent removal from the skin may aid in reducing microbial contact (e.g. bacteria, fungi) and limit conducive growth conditions under humid environments. As well as providing an inhospitable microclimate for microorganisms, the formation and removal of small droplets may also potentially aid in other areas such as reduction and cleaning of some surface contaminants consisting of single or multiple aggregates of particles.

This study shows that condensation on the hierarchically structured lotus leaf can facilitate self-propulsion of water droplets off the surface. Droplets on leaves inclined at high angles can be completely removed from the surface by self-propulsion with the assistance of gravity. Due to the small size of mobile droplets, light breezes may also fully remove the propelled droplets, which are typically projected beyond the boundary layer of the leaf cuticle. Moreover the self-propelled droplets/condensate were able to remove contaminants (eg silica particles) from the leaf surface. The biological significance of this process may be associated with maintaining a healthy cuticle surface when the action of rain to clean the surface via the lotus effect is not possible (due to no precipitation). Indeed, the native lotus plants in this study were located in a region with extended time periods (several months) without rain. Thus, dew formation on the leaf may provide an alternative self-cleaning mechanism during times of drought and optimise the functional efficiency of the leaf surface as well as protecting the surface from long term exposure to pathogens such as bacteria and fungi.

Black silicon is a synthetic nanomaterial that contains high aspect ratio nanoprotrusions on its surface, produced through a simple reactive-ion etching technique for use in photovoltaic applications. Surfaces with high aspect-ratio nanofeatures are also common in the natural world, for example, the wings of the dragonfly Diplacodes bipunctata. Here we show that the nanoprotrusions on the surfaces of both black silicon and D. bipunctata wings form hierarchical structures through the formation of clusters of adjacent nanoprotrusions. These structures generate a mechanical bactericidal effect, independent of chemical composition. Both surfaces are highly bactericidal against all tested Gram-negative and Gram-positive bacteria, and endospores, and exhibit estimated average killing rates of up to ~450,000 cells min⁻¹ cm⁻². This represents the first reported physical bactericidal activity of black silicon or indeed for any hydrophilic surface. This biomimetic analogue represents an excellent prospect for the development of a new generation of mechano-responsive, antibacterial nanomaterials.

The microstructures on elytral surface of aquatic beetles belonging to Hydrophilidae and Dytiscidae were observed under an environment scanning microscope, and the wettabilities were determined with an optical contact angle meter. The results show the elytral surfaces are relatively smooth compared to the structures of other insects such as the butterfly wing scales or cicada wing protrusions. They exhibit a polygonal structuring with grooves and pores being the main constituent units. The contact angles (CAs) range from 47.1^o to 82.1^o. The advancing and receding angles were measured by injecting into and withdrawing a small amount of water on the most hydrophilic (with a contact angle of 47.1^o) and hydrophobic (with a contact angle of 82.1^o) elytral surfaces, which illustrates the vital role of three-phase contact line (TCL) in the wetting mechanism of aquatic beetle elytral surfaces.