Lokman Uzun

Background: Glioblastoma (GBM) remains difficult to treat, and natural bioactive formulations are being explored as experimental modifiers of tumor-associated cellular processes. This study examined a fixed-ratio olive polyphenol–bromelain formulation in U87MG cells and performed targeted phenolic characterization of its olive-derived component. Methods: Seven phenolics were quantified by LC–MS/MS. U87MG cells were exposed to the formulation or a bromelain comparator. The MTT metabolic signal, DNA-content cell-cycle distribution, IL-6, IL-10, and extracellular total antioxidant capacity (TAC) were assessed. Results: The total quantified phenolics were 483.22 mg/kg, with oleocanthal, oleuropein, and 3,4-DHPEA-EDA predominating. The formulation reduced the MTT signal more than bromelain at lower and intermediate concentrations, although the response became non-monotonic at higher concentrations. Combination-treated cells showed greater G0/G1 accumulation. Culture supernatants showed lower IL-6 and higher IL-10 and TAC relative to untreated controls. Conclusions: The formulation was associated with changes in metabolic activity, cell-cycle distribution, and extracellular cytokine/redox readouts in U87MG cells. Because no polyphenol-only arm, direct cell-death assay, complete replicate-level inferential analysis, or non-tumoral comparator was available, the findings are descriptive and do not establish synergy, cytotoxicity, mechanism, tumor selectivity, or therapeutic efficacy.

Deoxynivalenol (DON) is one of the most common mycotoxins found in food and feed. This study aims to address the growing need for sensing platforms by applying surface engineering for the high-sensitivity detection of DON. Antibody immobilization via a self-assembled monolayer (SAM) strategy was systematically compared with immobilization on a porous poly(glycidyl methacrylate) [poly(GMA)] surface. The developed surfaces were characterized using Fourier transform infrared spectroscopy - attenuated total reflection (FTIR-ATR), atomic force microscopy (AFM), contact angle (CA) measurements, cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The results confirmed successful surface modification and demonstrated that surface functionalization plays a critical role in sensor performance and signal transduction. DON in standard solutions was determined using the developed AuSPE/Cys@antiDON and AuSPE/poly(GMA)@antiDON sensor platforms, with a linear working range of 2–20 ng/mL. The limit of detection (LOD) and limit of quantification (LOQ) for standard solutions were 1.43–4.33 ng/mL for AuSPE/Cys@antiDON and 1.67–5.07 ng/mL for AuSPE/poly(GMA)@antiDON, respectively. The recovery values obtained for the wheat sample were 103.72% and 99.76%, respectively. The developed sensor exhibited high selectivity, sensitivity, and repeatability performance for DON determination in both standard solutions and wheat matrix. Graphical Abstract The alternative text for this image may have been generated using AI.

In this study, human fetal osteoblasts behavior was investigated on titanium surfaces that has been modified with amino acid conjugated self-assembled molecules. For this purpose, 3-aminopropyltriethoxysilane (APTES) was conjugated by histidine and leucine and these newly synthesized molecules were used in different combinations to modify titanium surfaces via creating amino acid conjugated self-assembled monolayers (SAM) on titanium surfaces. The modification of the surfaces to introduce hydrophilic and hydrophobic regions on the surface was achieved with varying concentrations (v/v,100:0 20:80, 50:50, 80:20, 0:100). X-ray photoelectron spectroscopy (XPS) analysis and water contact angle measurements were performed for characterizing all of the modified surfaces in order to verify presence of amino acid specific bonds and wettability behavior to find suitable concentrations to support initial cell adhesion. In order to confirm that the surface modification supported cell adhesion and proliferation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed. Our results have shown that, amino acid SAM modification can be used to fine tune surface wettability and adherent cells were able to proliferate at different rates using different mixture concentrations. This presented approach can prove useful for expanding fine tuning surface chemistry methods for more specific applications and research.

Nanobubbles are designed to increase structural stability and enhance the distribution of the transported drug to the targeted site.