Jiming Hu

Multiplexed vibrational imaging holds great potential for tissue molecular analysis, yet fully standardized workflows suitable for routine clinical histopathology remain scarce. Herein, we report a clinically applicable methodology using monodisperse triple-bond Raman polymer nanoparticles (∼50 nm). This approach integrates one-step labeling and single-scan acquisition to achieve high-performance multiplexed Raman imaging of 5 protein markers in hepatocellular carcinoma (HCC) tissues. Targeting hepatitis B virus (HBV)-associated HCC, our biomarker panel combines the viral protein HBx with 4 clinically used indicators: AFP, GS, GPC3, and CD34. We further reveal a clinically critical dissociation: several high-grade HBV-HCC specimens exhibit markedly elevated oncogenic markers with comparatively low HBx expression, consistent with antiviral therapy suppressing viral protein production without reversing tumor aggressiveness. Taking advantage of the ultranarrow, well-resolved signals generated by triple-bond tags within the biological silent region (1800−2800 cm−1), we achieve high-fidelity spatial mapping and quantitative analysis. Unlike conventional pathological methods relying on morphological observation, clinical manifestations, or single-marker detection, this technique simultaneously evaluates viral etiology, tumor progression, and metastatic potential, effectively reducing misclassification risk in patients with discordant viral and oncogenic profiles, and completes the entire assay within approximately 24 h. This study establishes a rapid and reliable histopathological platform that integrates pathogen identification and comprehensive cancer characterization, demonstrating substantial value for both cancer research and clinical diagnostics.

Excessive oxidative stress and chronic inflammation are key obstacles to diabetic foot ulcer (DFU) healing. However, indiscriminate scavenging of reactive oxygen species (ROS) can disrupt physiological redox signaling that supports tissue repair. Here, we present a flexible feedback-guided sensing-and-treating patch integrating a ratiometric surface-enhanced Raman scattering (SERS) probe for real-time H₂O₂ monitoring with a photothermal-triggered dimethylthiourea (DMTU) release system. The SERS probe enables quantitative detection of H₂O₂ through a phenylboronic acid reporter, while near-infrared (808 nm) irradiation precisely triggers on-demand antioxidant delivery to restore physiological H₂O₂ levels. In diabetic mouse models, this hybrid patch effectively reduced oxidative stress, modulated inflammatory cytokines, promoted angiogenesis and collagen deposition, and markedly accelerated wound closure without adverse systemic effects. This work establishes a monitoring-guided redox-regulation strategy for intelligent, responsive wound management and highlights the potential of SERS-based theranostic materials in advanced biomedical applications.

Silver ion (Ag⁺) is a highly toxic metal ion, and its monitoring in water or food resources has become extraordinarily necessary within the scope of human health. In the light of the fact of Ag⁺-induced folding structure of specific peptides, an unlabeled and high-selectivity Ag⁺ assay is presented by means of intrinsic fluorescence of peptides. Under the quenching effect of gold nanoparticles (AuNPs), characteristic fluorescence of peptides could be considerably reduced by rapid modification. Along with the Ag⁺ adding, the fluorescence signals of peptide-AuNPs are largely enhanced by the behavior between peptides and Ag⁺. This is basically involving the formation of 4-coordinated complexes, generating the changes of peptides in structure and fluorescence properties. Under this circumstance, the adverse influence of plenty of interfering ions is suppressed, including the toxic Hg²⁺, Pb²⁺. The results highlight that Ag⁺ ions could be selectively recognized as low as 2.4 nmol/L with a linear range of 5 to 800 nmol/L. In comparison with other programs, the given approach declares simplicity, sensitivity, and superior selectivity. Furthermore, the biosensor excels in the practical application in water samples (e.g., lake, tap and drinking water) owing to its non-interference and on-site rapid determination.