Xingxiang Wang

Cadmium (Cd) speciation dynamics during vascular transport in peanut plants remain poorly defined. This study combined ¹¹³Cd isotope tracing with multiple separation techniques and targeted mass spectrometry to characterize Cd speciation across four developmental stages. Cd was primarily sequestered by proteins and pectates, limiting its vascular mobility. In the xylem, Cd²⁺ and low-molecular-weight complexes were detected, with cysteine (Cys)-Cd dominating in the seedling (40.8%) and maturity (26.3%) stages. Cd²⁺ increased to 43.9-47.8% during flowering and podding, while Cys-Cd declined below 10%. Phloem sap contained 83.5-88.6% organic Cd, including high-molecular-weight complexes (1.8-17 kDa) comprising 14.5-25.2% of total Cd. These complexes were absent in the xylem. Metabolomics indicated that amino acids in the phloem preferentially bound Cd, promoting its accumulation in seed-storage proteins. These findings clarify the dynamic speciation and vascular distribution of Cd in peanuts and provide insight into the biochemical mechanisms underlying its accumulation in seeds.

Boron, a crucial element for plant growth, has been demonstrated to mitigate cadmium (Cd) absorption in rice seedlings. However, its impact on Cd accumulation in rice grains and the underlying regulatory mechanisms remain poorly understood. The current study explored the roles of boron in reducing Cd accumulation and promoting ripening in rice through pot and hydroponic experiments. The results revealed that the basal boron application (1.5 mg kg(-1)) decreased grain Cd concentration by 61.1%, primarily due to the synergistic effects of inhibited Cd uptake and transport, along with increased maturation. Boron mitigated the root Cd2+ influx by 32.4% and transport factors by 36.0-47.3% primarily by downregulating the expression of OsNramp5, OsIRT1, and OsHMA2. Moreover, boron enhanced the activities of key sucrose-metabolizing enzymes and increased the relative expression levels of genes associated with sugar metabolism and transport, thereby shortening the rice growth period from 132 to 120 d. Field experiment confirmed that boron application decreased rice grain Cd concentration by 47.7% while promoting earlier maturation. This study elucidates the mechanism behind boron's ability to lower grain Cd levels and highlights its potential as an effective agronomic approach to mitigate food safety risks in rice grown on Cd-contaminated paddy soils.

In developing countries, like Pakistan, the pursuit of urbanization and economic development disrupts the delicate ecosystem, resulting in additional biogeochemical emissions of heavy metals into the human habitat and posing significant health risks. The levels of these trace elements in humans remain unknown in areas at higher risk of pollution in Pakistan. In this investigation, selected trace metals including Copper (Cu), Chromium (Cr), Lead (Pb) Cadmium (Cd), Cobalt (Co), Nickel (Ni), and Arsenic (As) were examined in human hair, urine, and nail samples of different age groups from three major cities (Muzaffargarh, Multan, and Vehari) in Punjab province, Pakistan. The results revealed that the mean concentrations (ppm) of Cr (1.1) and Cu (9.1) in hair was highest in Muzaffargarh. In urine samples, the mean concentrations (μg/L) of Co (93), As (79), Cu (69), Cr (56), Ni (49), Cd (45), and Pb (35) were highest in the Multan region, while As (34) and Cr (26) were highest in Vehari. The mean concentrations (ppm) of Ni (9.2), Cr (5.6), and Pb (2.8), in nail samples were highest in Vehari; however, Multan had the highest Cu (28) concentration (ppm). In urine samples, the concentrations of all the studied metals were within permissible limits except for As (34 µg/L) and Cr (26 µg/L) in Vehari. However, in nail samples, the concentrations of Ni in Multan (8.1 ppm), Muzaffargarh (9 ppm), Vehari (9.2 ppm), and Cd (3.69 ppm) in Muzaffargarh exceeded permissible limits. Overall, the concentrations of metals in urine, nail, and hair samples were higher in adults (39–45 age group). Cr, Cu, and Ni revealed significantly higher concentrations of metals in hair and water in Multan, whereas As in water was significantly (p < 0.001) correlated with urinary As in Multan, indicating that the exposure source was region-specific.