Scott W. Pryor

While the production costs and logistical benefits of biomass pelleting have been widely discussed in the literature, the downstream economic and environmental benefits of processing pelleted biomass have been largely neglected. To investigate those benefits, we performed a comparative techno-economic analysis and life cycle assessment of producing ethanol using loose and pelleted forms of biomass. Analyses of a 2000 metric tons (dry)/d biorefinery showed that using pelleted biomass is more economical than using loose or baled biomass. The lowest minimum ethanol selling price (MESP) for pelleted biomass was USD 0.58/gal less than the lowest MESP for loose biomass. Among all processing conditions analyzed, MESP for ethanol produced with pelleted biomass was always lower than when produced with loose biomass. Shorter pretreatment and hydrolysis times, higher pretreatment solids loadings, lower ammonia requirements, and reduced enzyme loadings were the primary factors contributing to lower MESP with pelleted biomass. Similarly, pelleted biomass also demonstrated a 50% lower life cycle greenhouse gas emission compared to loose biomass. Emissions from higher pelleting energy were offset by downstream advantage in lower chemical needs.

Cellulosic biorefineries have attracted interest due to the growing energy security and environmental concerns related to fossil fuel-based energy and chemicals. Using pelleted biomass as a biorefinery feedstock can reduce their processing inputs while improving biomass handling and transportation. However, it is still questionable whether energy and emission savings from feedstock transportation and processing can justify pelletization. A life cycle assessment approach was used to compare energy consumption and greenhouse gas (GHG) emissions from pelleted and non-pelleted corn stover as a biorefinery feedstock. Operations considered were pelleting, transportation, and soaking in aqueous ammonia (SAA) pretreatment. Despite greater GHG emissions (up to 25 times higher than the transportation) generated from the pelleting process, the model showed a significant opportunity to offset and even reduce overall GHG emissions considering the pretreatment process benefits. Our process energy analysis showed that SAA pretreatment of pelleted biomass required significantly lower energy inputs (56%) due to the lower-severity pretreatment’s effectiveness. Higher pretreatment solid loadings are allowed when pelleted biomass is used and this reduces the process chemicals and water requirements by 56% and 49%, respectively. This study demonstrated that the SAA pretreatment of pelleted biomass might be a feasible option as the cellulosic biorefinery feedstock.

The enzymogel has revealed unique properties making it a novel type of phase-boundary biocatalyst. This chapter discusses the application of cellulase (CEL) enzymes to cleave cellulose chains and transform them to glucose which could be used for the production of biofuels or biochemicals. A multicycle hydrolysis of cellulose could be realized using both mechanisms of enzymogel bioactivity: multiple release and uptake of CEL by recyclable enzymogel or multiple use of the enzymogel with no release of CEL. One of unique and interesting aspect of enzymogel particles is related to magnetic field directed bioconversion process. High enzyme loadings make the use of nanoparticles more efficacious as long as a biocatalytic activity of the enzyme does not decrease significantly at increased loadings. The concentrations of glucose and total reducing carbohydrates after partial biocatalytic cleavage of cellulose with free enzymes and enzymogel nanoparticles were monitored to reveal the impact of pH and temperature.