Ruchele Dias Nogueira

Introdução: O tratamento ortodôntico, embora benéfico para corrigir desalinhamentos dentários, pode alterar o ambiente oral influenciando a saúde periodontal. Objetivos: Avaliar os parâmetros clínicos periodontais e analisar as alterações microbianas no biofilme subgengival antes (T0) e após seis meses (T2) de tratamento ortodôntico, em pacientes com periodontite controlada (GDP) e comparar com indivíduos saudáveis (GC). Materiais e Métodos: O estudo incluiu dez pacientes com GDP e dez controles saudáveis (GC). Os parâmetros clínicos foram medidos em duas visitas. Amostras de biofilme foram coletadas e analisadas para patógenos periodontais: Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Treponema denticola (Td) e Tannerella forsythia (Tf) usando PCR em Tempo Real. Resultados: No grupo GDP, a profundidade de sondagem diminuiu significativamente entre as visitas (p<0,05). Tf foi o patógeno mais frequentemente detectado em ambas as visitas, enquanto as outras bactérias foram detectadas em menos de 30% das amostras. No T2, Pg não foi detectado e Aa foi encontrado em apenas uma amostra, enquanto as detecções de Fn e Td aumentaram. No grupo GC, menos de 40% das amostras mostraram Pg, Aa e Fn no T1, com Tf e Td sendo mais comuns. No T2, a detecção de Fn e Tf aumentou, enquanto Aa e Td diminuíram. Nenhuma diferença significativa foi encontrada entre os grupos ou visitas (p<0,05). Conclusão: O tratamento ortodôntico parece influenciar positivamente a saúde periodontal em pacientes com periodontite controlada, como mostrado pela redução na profundidade de sondagem. As mudanças microbianas sugerem que, enquanto alguns patógenos persistem, outros, como P. gingivalis, diminuem com o melhor manejo periodontal.

Highlights ■ Candidiasis can present as localized or systemic infections. Disseminated infections in newborns and adults can be life-threatening, with high mortality and morbidity rates (40–60%), and rank as the fourth most common type of nosocomial infection. ■ Oral candidiasis is a local condition affecting 10-15% of children in their first months of life. ■ Saliva and 3’-sialyllactose can disrupt the initial development of Candida albicans biofilm. ■ The effect of colostrum needs to be elucidated because it may hinder

Highlights ■ Candidiasis can present as localized or systemic infections. Disseminated infections in newborns and adults can be life-threatening, with high mortality and morbidity rates (40–60%), and rank as the fourth most common type of nosocomial infection. ■ Oral candidiasis is a local condition affecting 10-15% of children in their first months of life. ■ Saliva and 3’-sialyllactose can disrupt the initial development of Candida albicans biofilm. ■ The effect of colostrum needs to be elucidated because it may hinder

To verify whether an infiltrant resin used to inactivate white spot lesions (WSL) on enamel can resist the abrasive challenges promoted by tooth brushing with different dentifrices.

Introduction: Fixed orthodontic appliances can lead to enamel demineralization and gingival changes due to increased biofilm. Aim: To detect the presence of Prevotella intermedia, Tannerella forsythia, Streptococcus mutans, Scardovia wiggsiae, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Porphyromonas gingivalis in oral biofilm and presence of white spot lesions and gingivitis in users of self-ligating and conventional brackets. Design: This observational controlled study was conducted from January to December 2020. The participants used fixed orthodontic therapy for 6 months and were divided into a conventional and a self-ligating bracket group. The participants underwent clinical examination, and biofilm samples were collected from their lower incisors. Results: No differences were found in the bacteria detection between the groups (p>0.05). However, white spot lesions were more common in users of self-ligating brackets (p=0.019). There was no association between clinical data and the detection of any microorganism (p>0.05). The fluorescence intensity of A. actinomycetemcomitans was higher in self ligating brackets than in conventional brackets (p<0.05). Implications: The microbial diversit did not differ between the types of brackets; however, the presence of white spot lesions and the amount of A. actinomycetemcomitans were higher in patients with self-ligating brackets.

Aim: To assess and compare the inflammatory infiltrate, area of muscle cells, microvessel density (MVD) and expression of cytokines in the myocardium (MM) and lingual (LM) musculature between chagasic and non chagasic individuals. Methods: Fragments of MM and LM from autopsied chronic chagasic (CC) (n=18) and non chagasic individuals (n=24) were histologically processed for histochemical and immunohistochemical evaluation. Was assessed the MVD by CD31 and quantification of immune cells infiltrated in MM and LM, and also expression of IL-1, IL-10 and TGF- β1. Results: There were higher intensity of inflammation and larger area of muscle cells in the myocardium and tongue of chagasic compared to non-chagasic individuals (p<0.0001). In the chagasic individuals, there was a positive correlation: regarding to the MVD between the LM and MM (p=0.049); between area of MM and density of leukocytes expressing TGF-β1 (p=0.049); and IL-10 correlate with the MM and LM (p=0.029). Conclusions: The correlation between the density of leukocytes immunostained by anti-TGF-β1 and hypertrophy in the myocardium suggests the participation of this cytokine in cardiac muscle hypertrophy. Moreover, the hypertrophy in the myocardium and lingual muscles, and the concomitant expression of CD31 and IL-10 between the myocardium and lingual musculature in chagasic individuals suggests that evaluation of the tongue musculature presents certain similarities changes as a myocardium muscle in CC. However, it needs more studies to verify in other phases of the disease, if the parasite is installed in the tongue or if the mechanisms are reflex of systemic changes in level of immune response.

To evaluate the presence of Streptococcus mutans (SM), main pathogen of dental caries, interleukin-6 and -10 in samples of amniotic fluid (AF) and clinical and oral data of the healthy pregnancy, t...

Although implant rehabilitations are successful, a small portion of patients may present peri-implant mucositis and peri-implantitis due to the bacteria biofilm formation. The purpose of the present study was to evaluate the presence of Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Fusobacterium nucleatum (Fn) and Scardovia wiggsiae (Sw) in Peri-implant gingival Fluid (PGF) of patients who received implants 12 months ago and associated with periodontitis (PD) history and clinical data. Samples were collected from 44 volunteers and analyzed by PCR with specific primers. The results showed that Pg were detected in 34%, Fn and Pi, 81.8%, and Sw 75% of the volunteers. Pg was frequently detected in patients who had a history of PD (p0.05, q