Felix Adrion

Time series analysis can facilitate the detection of complex behavioral patterns and potentially provide new opportunities to assess animal welfare. The aim was to investigate whether dairy cows exhibit daily, individual patterns in activity and in area use in the barn. We predicted that behavioral patterns will be more consistent (1) within than between cows, (2) when area categorization is more specific and, thus, allows the detection of individual preferences for areas, and (3) during the night. We conducted the study at an experimental farm with 20 lactating Brown Swiss and Swiss Fleckvieh cows. The animals were housed in cubicles, and they received feed and were milked twice daily. Activity was recorded with IceTag pedometers (IceRobotics Ltd.), and area use with the SMARTBOW sensor system (Zoetis). Data were collected for 55 consecutive days and analyzed at 1-min intervals. To investigate the behavioral time series, we performed a hierarchical clustering analysis. A clustering process calculated distances between days, which were compared within and between cows based on t-tests and analyses of variance. Dendrograms of activity and area use showed that days of individual cows could not be grouped more closely together than those of different cows. A slightly better grouping was achieved with a more specific area categorization, but not during a specific time period. However, the average distances between days were always smaller within (mean ± SD; activity: 95.62 ± 76.88, lying areas: 0.14 ± 0.03, functional areas: 0.12 ± 0.01) than between cows (activity: 109.62 ± 75.33, lying areas: 0.16 ± 0.02, functional areas 0.13 ± 0.01). Considering that the time series of individual cows were slightly but always more similar compared with those between cows, and that more consistent patterns were found when the area categorization was more specific, it can be concluded that the cows exhibited weak individual preferences in area use and also weak daily individual patterns in activity and area use. Because the visual exploratory and empirical approaches used in this study do not account for variability, they do not seem to be suitable for the detection of patterns in animals that display greater plasticity in their temporal structure of activity. Thus, although determining the temporal structure of activity and area use bears the potential to assess the behavior and, in turn, for example, the physiological state and health status of cows, it does not seem to be achievable with a cluster analysis. Therefore, time series methods that account for temporal fluctuations in behavior should be further explored.

The optimal milking cluster should milk as gently as possible to minimize the mechanical effect on the teat tissue at an optimal milking performance and milk quality. The objective of this study was to investigate the influence of liner shape (round vs. triangular) and type of cluster ventilation (claw vs. mouthpiece chamber; MPC) on milking performance and vacuum at the teat end and in the MPC. Our hypothesis was that liner shape and cluster ventilation affect milking performance and MPC vacuum. Six Holstein Friesian cows were milked twice daily over 12 d with a bucket milker, using 4 different cluster types that combined liner shape and type of cluster ventilation at 3 different system vacuum settings (35, 42, and 50 kPa) in an incomplete randomized block design. Milk flow and vacuum in the MPC, at the teat end (measured in the short milk tube), and in the short pulse tube were continuously recorded during milking. Milk flow was higher, and hence main milking time was shorter, with the round than with the triangular liners. The MPC vacuum was lower in round than triangular liners, which was caused by higher air leakage between teat and liner barrel in the triangular liners. The MPC vacuum, as well as its cyclic fluctuations, increased at the end of milking (immediately before cluster detachment) in all cluster types, with the highest amplitude of fluctuation in the triangular liners with MPC ventilation. The MPC ventilation reduced the MPC vacuum in both liner types at the end of milking, and also in triangular liners during peak milk flow. Despite the observed differences of MPC vacuum, the ventilation type did not affect milking performance. However, milking with triangular MPC-ventilated liners caused an increased proportion of foamed milk, which could potentially have a negative effect on milk quality.

Feeding behaviour can be used as an important indicator to support animal management. However, using feeding behaviour as a tool for dairy cow management an automatic sensor system is needed. Hence, the objective of this study was to setup, test and validate a ultra-high frequency (UHF) radio-frequency identification (RFID) system for measuring time dairy cows spent at the feed fence using two types of passive UHF ear tags. In a first experiment, the reading area of the system was evaluated in two antenna positions. Subsequently, the UHF RFID system was validated with video observations and compared to the measurements of chewing time of a noseband pressure sensor and of the time spent at the feed fence registered by a sensor system with real-time localisation. Differences in the reading area were detected between the two antenna positions and types of ear tag. The antenna position leading to less false positive registrations was chosen for the experiment with cows. The validation with video data showed a high average sensitivity (93.7 ± 5.6%, mean ± standard deviation), specificity (97.8 ± 1.1%), precision (93.8 ± 2.3%) and accuracy (96.9 ± 0.9%) of the UHF RFID system for measuring the time spent at the feed fence. The comparison with the noseband pressure sensor and the real-time localisation resulted in high correlations with a correlation coefficient of r = 0.95 and r = 0.93, respectively. However, substantial absolute differences between the three systems pointed out differences between direct and indirect measures of feeding behaviour in general and between the different sensors in particular. Thus, detailed considerations are necessary before interpreting automatically measured feeding data generally.

The main objective of this paper is the monitoring of daily activity patterns of fattening pigs at different locations in the housing environment using UHF-RFID. Four hundred fattening pigs were equipped with UHF-RFID ear tags and monitored during the fattening period for about four months. The RFID antennas were installed at the feeding troughs, playing devices and drinkers. A validation phase for each of these locations was carried out prior to the first data collection. The sensitivity (true positive rate) of the UHF-RFID system was about 80% at the feeding trough and the playing device and about 60% at the drinkers. The mean of the daily visiting time of all pigs at the trough was about 55 min. The mean visiting duration at the playing device was about 38 min and at the drinkers about 9 min. The visiting times of the pigs showed a high intra- and inter-variability. It was observed that the average visit duration at the feeding trough decreases over the course of a fattening period but increases at the playing device. A documentation of visiting times of animals is possible utilizing RFID systems, allowing a higher data density than video or direct observations.

: Animal facilities are increasing in size, while the availability of skilled workers is decreasing, thus, making it difficult for the farm laborers to ensure the health and well-being of all animals under their care. Passive Radio Frequency Identification (RFID) systems have been successfully used in animal facilities and research has identified potential applications in behavior monitoring for automated illness detection. While RFID signals range in frequency from 9 kHz to 5.8 GHz, the three most common frequencies are Low Frequency (LF, 125 kHz or 134.2 kHz), High Frequency (HF, 13.56 MHz), and Ultra-High Frequency (UHF, 865-868 MHz or 902-928 MHz). The objective of this article is to compare and evaluate the application of these three different RFID systems within large research facilities for livestock and poultry in terms of hardware characteristics, system design, and data processing and usage. Differences in tag construction, availability and cost are evident, but also basic differences in reader and antenna function, such as physics of communication, speed of detection, and anti-collision procedures exist. The systems have significant differences in reading ranges and are known to have varying influence of materials, especially water and metal, on the performance of the systems. However, the data streams, as well as methods of data processing and the creation of events (e.g., visits to a feeder), are similar for all systems. The characteristics mentioned do not necessarily identify an ideal RFID technology but reveal positive and negative aspects of each system. The three different RFID systems have been successfully applied in livestock and poultry facilities. Current research is focused on the utilization of the RFID data in prediction and decision models for illness, animal welfare, and management actions. Keywords: Behavior, Cattle, Frequency ranges, Health and welfare, Poultry, Swine, Transponder.