Minghui Yang

The unique anisotropy of gold nanorods (Au NRs) endows them with two intense SPR absorption bands, making them widely used for constructing fluorescent probes that operate in a “quench-and-recover” mode. Nevertheless, most existing Au NR-based probes utilize only one SPR absorption to quench the fluorescence and thus do not fully exploit the quenching potential of Au NRs. Herein, we employ dye-sensitized upconversion nanoparticles (UCNPs) as the energy donor and harness both SPR absorptions to quench the emissions from the dye and the UCNPs, thereby achieving full utilization of the quenching capacity of Au NRs. A DNA-programmed nanosatellite assembly probe that integrates near-infrared (NIR) dye-sensitized UCNPs with Au NRs through DNA base pairing was designed. In the nanosatellites, the longitudinal SPR absorption quenches the NIR dye, suppressing dye sensitization and decreasing the emission of UCNPs, while the transverse SPR absorption quenches the emission of the UCNPs, enabling efficient quenching of the total emission of the dye-sensitized UCNPs. This dual-SPR quenching achieves a high quenching efficiency of 98.5%. In the presence of target miRNA, the nanosatellites disassemble into Au NRs and dye-sensitized UCNPs, restoring the luminescence of UCNPs, with a maximum signal-to-background ratio of 16.4 and a detection limit of 71 fM. The probe performs well in complex biological samples, offering a programmable strategy that takes full advantage of the anisotropic plasmonic properties of Au NRs for sensitive nucleic acid analysis.

A novel luminescent probe for trichloroacetic acid (TCAA) was constructed based on luminescence resonance energy transfer (LRET) between upconversion nanoparticles (UCNPs) and triangular silver nanoprisms (AgNPR).

A complementary dual-mode biosensor was constructed for determination of chlorpyrifos (CP) using a multifunctional composite consisting of MnO₂@thionine/ZIF-8 (MnO₂@Thi/ZIF-8). This composite synergistically combines the good oxidase-mimetic activity of MnO₂ dispersed on the surface of ZIF-8 with the stable electroactivity of thionine (Thi) encapsulated within detachable ZIF-8 framework, enabling dual-mode detection of CP. In the presence of acetylcholinesterase (AChE) and its substrate acetylthiocholine (ATCh), enzymatic hydrolysis quantitatively produces acetic acid (HAc) and thiocholine (TCh). TCh acts as a reducing agent to convert MnO₂ to Mn²⁺, leading to diminished oxidase-mimetic activity of MnO₂ and a subsequent decrease in the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB). Concurrently, HAc induces the hydrolysis of ZIF-8, resulting in the release of encapsulated Thi, generating electrochemical current. Upon CP addition, AChE activity is selectively inhibited, reducing the production of both TCh and HAc. The complementary dual-mode sensor exhibits good analytical results (electrochemical mode: 0.1-60 ng/mL; colorimetric mode: 0.5-60 ng/mL) and satisfactory performance in real-sample analysis. This study not only provides an effective strategy for sensitive and accurate monitoring of OPs through the synergistic integration of inorganic oxides with metal-organic frameworks (MOFs) but also offers new insights into the design of multifunctional nanozymes for multimodal sensing platforms.

Postoperative tumor management faces persistent challenges, including residual tumor survival, immune suppression, and impaired wound healing. Surgical resection eliminates the primary lesion but simultaneously removes the continuous antigen source required for sustained immune activation, leaving the postoperative microenvironment vulnerable to recurrence and metastasis. Here, a flexible wearable cold-catalytic patch is constructed by integrating thermoelectric nanorods, enzymatic components, and a zwitterionic hydrogel, enabling synergistic immune activation and enhanced tissue repair. Localized cold stimulation triggers thermoelectrocatalytic generation of reactive oxygen and nitrogen species, enabling sustained release of bioactive molecules that promote antigen presentation, amplify inflammatory cytokine secretion, and enhance immune cell infiltration. Concurrently, the thermoelectrical cues and NO signaling produced during cold catalysis stimulate fibroblast migration, angiogenesis, and extracellular matrix remodeling, thereby accelerating postoperative wound closure. In vivo studies demonstrate that this platform effectively suppresses residual tumor proliferation and distant metastasis while markedly improving healing quality. This work reports a unified thermoelectrocatalytic strategy that couples immune cascade activation with enhanced tissue repair, offering a broadly applicable paradigm for next-generation postoperative tumor therapy.

A novel tyrosinase probe (SCy-tyr) was developed. This probe enables highly sensitive and specific imaging of tyrosinase in living cells within 50 minutes, providing a new tool for precise imaging and surgical resection of melanoma.