Selim Misirlioglu

Supplementary Figure 4 shows IP-10 (CXCL10) expression levels in drug-treated cell lines.

Supplementary Table 4 shows enriched gene sets upregulated in CAOV3 cells treated with veliparib .

Supplementary Figure 1 shows clustering of drug-treated cells compared to controls.

Supplementary Figure 2 shows Venn diagrams of elevated genes in drug-treated cells.

Supplementary Figure 3 shows CXCL10 gene and protein expression levels in drug-treated cell lines.

Supplementary Table 3 shows enriched gene sets upregulated in OVCAR3 cells treated with veliparib .

Supplementary Table 2 shows enriched gene sets upregulated in OVCAR3 cells treated with talazoparib.

Supplementary Table 1 shows enriched gene sets upregulated in CAOV3 cells treated with talazoparib.

Pro-inflammatory genes are significantly upregulated in CAOV3 and OVCAR3 talazoparib-treated cells compared with control and veliparib-treated cells. Volcano plots highlighting fold change values of a small set of pro-inflammatory genes (FDR < 0.1) in (A and C) talazoparib-treated vs. DMSO-treated cells or (B and D) veliparib-treated vs. DMSO-treated cells. Red circles denote genes that were significantly upregulated, and blue circles represent genes that were significantly downregulated. Results shown are from three independent experiments. E–H, GSEA plots representative of the top upregulated pathway in each of the four comparisons. NES, normalized enrichment score.

IL-8 (CXCL8) is exclusively secreted in BRCA1 methylated or mutated HGSC cells after olaparib treatment but is secreted in all HRD HGSC cells after talazoparib treatment. A, OVCAR3, (B) OVCAR8, and (C) COV362 cells were treated with DMSO, 10 μmol/L veliparib, 10 μmol/L olaparib, or 2 μmol/L talazoparib for 48 hours. The supernatants were then collected, and IL-8 protein expression was analyzed by an ELISA assay. A four-parameter logistic curve fit was used to analyze these results. T tests were performed between DMSO- and the individual PARPi–treated groups. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not statistically significant.