Helder Mateus Viana Espirito Santo

Hydropower dams affect freshwater biodiversity by virtue of modifying flow regimes and degrading habitat conditions both above and below the water impoundment and diversion structure. In recent decades, there has been a global proliferation of Small Hydropower Plants (SHPs) propelled by incentive policies and a general perception that small dams are less harmful to the environment. However, limited science is available on the effects of SHPs on freshwater biodiversity, which is concerning considering their sheer numbers and the high variability in the size and operation of dams classified as SHPs. We investigated the ecological effects of 12 SHPs on macroinvertebrate and fish assemblages of the Chapecó River Basin, Brazil. Our study included sites located upstream of the SHPs and sites directly affected by their operations (i.e., reservoir, dewatering section and downstream of the powerhouse). This design allowed us to quantify shifts in taxonomic composition and examine the relative role of different SHP attributes (e.g., dam height, reservoir area, age) in explaining these changes. Our results reveal that SHPs modify the composition of both macroinvertebrate and fish assemblages, but the magnitude of such changes vary widely among dams. Both assemblages exhibited directionality in taxonomic shifts, resulting in a gradual replacement of taxa associated with fast‐flowing habitats by other groups more commonly associated with standing waters. We also found that changes in assemblage structureare related to structural (dam height, reservoir area) and spatial attributes (distance from headwaters) of SHPs, although the predictive power of these attributes varied taxonomically and among reservoir, dewatering and downstream sites. Overall, our results demonstrate that SHPs have important and heterogeneous effects on freshwater assemblages, which may manifest individually or cumulatively across the riverscape. This suggests that it is unlikely that a priori classifications of dams based on their structural attributes can replace site‐specific and cumulative impact assessments. The results also shed light on the need for better understanding of how SHP attributes shape their ecological effects, so that a full assessment of the potential environmental impacts of the wide range of "small" dams is possible.

Different mating systems are expected to affect the extent and direction of hybridization. Due to the different levels of sexual conflict, the weak inbreeder/strong outbreeder (WISO) hypothesis predicts that gametes from self-incompatible (SI) species should outcompete gametes from self-compatible (SC) ones. However, other factors such as timing of selfing and unilateral incompatibilities may also play a role on the direction of hybridization. In addition, differential mating opportunities provided by different mating systems are also expected to affect the direction of introgression in hybrid zones involving outcrossers and selfers. Here, we explored these hypotheses with a unique case of recent hybridization between two mangrove killifish species with different mating systems, Kryptolebias ocellatus (obligately outcrossing) and K. hermaphroditus (predominantly self-fertilizing) in two hybrid zones in southeast Brazil. Hybridization rates were relatively high (~20%), representing the first example of natural hybridization between species with different mating systems in vertebrates. All F1 individuals were sired by the selfing species. Backcrossing was small, but mostly asymmetrical with the SI parental species, suggesting pattern commonly observed in plant hybrid zones with different mating systems. Our findings shed light on how contrasting mating systems may affect the direction and extent of gene flow between sympatric species, ultimately affecting the evolution and maintenance of hybrid zones.

Mangrove killifishes of the genus Kryptolebias have been historically classified as rare because of their small size and cryptic nature. Major gaps in distribution knowledge across mangrove areas, particularly in South America, challenge the understanding of the taxonomic status, biogeographical patterns and genetic structuring of the lineages composing the self‐fertilizing ‘Kryptolebias marmoratus species complex’. Here, we combined a literature survey, fieldwork, and molecular data to fill major gaps of information about mangrove killifishes distribution across western Atlantic mangroves. We found that selfing mangrove killifishes are ubiquitously distributed across the Caribbean, Central, and South American mangroves and report 14 new locations in South America, extending the range of both the ‘Central clade’ and ‘Southern clade’ lineages which overlap in the Amazon. Although substantial genetic differences were found between clades, we also found further genetic structuring within clades, with populations in Central America, North, and Northeast Brazil generally showing higher levels of genetic diversity compared to the clonal ones in southeast Brazil. We discuss the taxonomic status and update the geographical distribution of the Central and Southern clades, as well as potential dispersal routes and biogeographical barriers influencing the distribution of the selfing mangrove killifishes in the western Atlantic mangroves.

Climate change is a major threat to biodiversity. Rainfall patterns over recent decades have changed and extreme events are intensifying. These are expected to affect the hydrological dynamics and structure of forest streams in the Amazon basin, which strongly depend on the local rainfall regime, with potential consequences for local fish assemblages. Here we used four repeated surveys of stream fish assemblages across 10 × 10 km area of a pristine forest reserve in central Amazonia over an 18‐year (2001–2018) period to investigate the temporal dynamics of fish taxonomic and functional composition in relation to changes in stream habitats and local climatic regime. We investigated changes in magnitude, direction, and congruence of fish assemblage trajectories over time. Total annual rainfall increased over time, with stormy days (i.e. daily rainfall above 20 mm) becoming more frequent during recent decades and a concomitant change of stream substrates from litter‐dominated to free sand. The overall taxonomic and functional composition of fish assemblages changed consistently over time, with stronger differences between the first and last surveys due to alterations in relative abundances of some of the most abundant species. Functional composition moved from open water species (compressed‐bodied species with less developed fins and terminal‐oriented mouths) to species adapted to lateral pools and shallow marginal areas (species with fusiform body shape, more developed fins, and superior‐oriented mouth) We provide evidence of a concerted directional change of fish assemblages associated with increased precipitation and a higher frequency of stormy days over the last 2 decades. Considering the short time interval and the subtleness of the climatic changes assessed in this study, as well as the historically assumed structural stability of Amazonian streams, these findings are surprising and indicate those stream fish assemblages may respond quickly to climate changes.

Different mating systems can strongly affect the extent of genetic diversity and population structure among species. Given the increased effects of genetic drift on reduced population size, theory predicts that species undergoing self-fertilisation should have greater population structure than outcrossed species; however, demographic dynamics may affect this scenario. The mangrove killifish clade is composed of the two only known examples of self-fertilising species among vertebrates (Kryptolebias marmoratus and Kryptolebias hermaphroditus). A third species in this clade, Kryptolebias ocellatus, inhabits mangrove forests in southeast Brazil; however, its mating system and patterns of genetic structure have been rarely explored. Here, we examined the genetic structure and phylogeographic patterns of K. ocellatus along its distribution, using mitochondrial DNA and microsatellites to compare its patterns of genetic structure with the predominantly selfing and often-syntopic, K. hermaphroditus. Our results indicate that K. ocellatus reproduces mainly by outcrossing, with no current evidence of selfing, despite being an androdioecious species. Our results also reveal a stronger population subdivision in K. ocellatus compared to K. hermaphroditus, contrary to the theoretical predictions based on reproductive biology of the two species. Our findings indicate that, although morphologically similar, K. ocellatus and K. hermaphroditus had remarkably different evolutionary histories when colonising the same mangrove areas in southeastern Brazil, with other factors (e.g., time of colonisation, dispersal/establishment capacity) having more profound effects on the current population structuring of those species than differences in mating systems.

Different mating systems can strongly affect the extent of genetic diversity and population structure among species. Given the increased effects of genetic drift on reduced population size, theory predicts that species undergoing self-fertilization should have greater population structure than outcrossed species, however demographic dynamics may affect this scenario. The mangrove killifish clade is composed of the two only known examples of self-fertilising species among vertebrates (Kryptolebias marmoratus and K. hermaphroditus ). A third species in this clade, K. ocellatus, inhabits mangrove forests in southeast Brazil, however its mating system and patterns of genetic structure have been rarely explored. Here, we examined the genetic structure and phylogeographic patterns of K. ocellatus along its distribution, using mitochondrial DNA and microsatellites to compare its patterns of genetic structure with the predominantly selfing and often syntopic, K. hermaphroditus . Our results indicate that K. ocellatus reproduces mainly by outcrossing across much of its known range, with no current evidence of selfing, despite being an androdioecious species. Our results also reveal a stronger population subdivision in K. ocellatus compared to K. hermaphroditus , contrary to the theoretical predictions based on reproductive biology of the two species Our findings indicate that, although morphologically similar, K. ocellatus and K. hermaphroditus had remarkably different evolutionary histories when colonising the same mangrove areas in south-eastern Brazil, with other factors (e. g. time of colonisation, dispersal/establishment capacity) having more profound effects on the current population structuring of those species than differences in mating systems.

Hybridisation is a major source of evolutionary innovation. However, several prezygotic and postzygotic factors influence its likelihood and evolutionary outcomes. Differences in mating systems can have a major effect on the extent and direction of hybridisation and introgression. In plants, epigenetic mechanisms help to stabilize hybrid genomes and contribute to reproductive isolation, but the relationship between genetic and epigenetic changes in animal hybrids is unclear. We analysed the extent of a unique case of natural hybridisation between two genetically distant mangrove killifish species with different mating systems, Kryptolebias hermaphroditus (self-fertilising) and K. ocellatus (outcrossing), and the methylation patterns of their hybrids. Hybridisation rate between the species ranged between 14% and 26%. Although co-existing parental species displayed highly distinct genetic (microsatellites and SNPs) and methylation patterns (37,000 differentially methylated cytosines), our results indicate that F1 hybrids are viable and able to backcross with parental species. Hybrids had predominantly intermediate methylation patterns (88.5% of the sites) suggesting additive effects, as expected from hybridisation between genetically distant species. Differentially methylated cytosines between hybrids and both parental species (5,800) suggest that introgressive hybridisation may play a role in generating novel genetic and epigenetic variation which could lead to species diversification. We also found a small percentage of non-additive epigenetic effects which might act as an evolutionary bet-hedging strategy and increase fitness under environmental change.

Studies of fish assemblages have demonstrated that the main channels of rivers contain ichthyofauna adapted to this environment. However, information regarding the effects of temporal and spatial variations on this ichthyofauna is scarce. Using data from benthic fish assemblages in a major tributary of the Amazon basin collected during two consecutive receding and two rising water seasons, we tested the hypothesis that there are marked variations in community composition between the receding and rising water seasons. An asymmetry in predictability was detected among samples from the receding and rising seasons. Predictability in terms of species composition was higher for receding than rising seasons. The continual disassembly and reassembly cycles (due to dispersal) of local communities across a spatially heterogeneous landscape could explain this difference. Depth and dissolved oxygen affected the distribution of some benthic fish species during the rising seasons. This study highlights the important contribution of marginal wetlands to the benthic ichthyofauna inhabiting the main channel of the Purus River, as well as other major Amazonian rivers.

Amphibious habits are present in a variety of fish and are allowed by a set of physiological, morphological and behavioural adaptations. The most intriguing question, however, is what motivates an aquatic organism to venture into the land? Unfavourable abiotic and biotic conditions in the aquatic environment have been reported as main stimuli for fish to emerge from water. However, few attempts have been made to disentangle the effects of different social contexts on the propensity of amphibious fishes to leave the water. Trying to separate the effects of predator threat, intraspecific competition and mate searching on overland movement of amphibious fish, we ran a mesocosm experiment with the Amazonian killifish Anablepsoides micropus. Overland movement was quantified based on fish leaps between artificial pools set in enclosures in a primary forest area. Males moved overland more frequently than females and their movement depended on the social context, being higher in the presence of predators and lower in the presence of females. Females, instead, showed context‐independent movement rates. This suggests that males move between pools searching for mates, stopping when successful in this search. On the other hand, occasional encounters with predators may force males to abandon the pool as a defence mechanism. Female aggressiveness towards the predator may influence its low movement in the presence of the predator. Such differential responses may play important ecological and evolutionary roles, allowing A. micropus to occupy a wide range of environmental conditions under varied ecological and social contexts.