Sheng-Chung Chen

Here, we report the complete genome sequence of Anaerocolumna sp. MB42-C2 isolated from a hot spring in Qingliu, Fujian, China. The genome comprises 6,437,239 bp with a G + C content of 36.49 mol% and provides a genomic basis for further species delineation and characterization of genomic features.

Here, we report the complete genome sequence of Tissierella sp. strain MB52-C2 (= BCRC 81439) isolated from Wuyi Mountain soil, the source region of Wuyi rock tea. The genome is 3,892,263 bp with 30.9% G + C content and supports species delineation and genomic analyses of taxonomic position and genomic features.

Here, we report the complete genome sequence of Actinomycetaceae bacterium strain MB13-C1-2, isolated from the feces of black soldier fly ( Hermetia illucens ) larvae, comprising 2,578,609 bp and a GC content of 57.23%. The genome was selected for further analyses of species delineation and genomic features.

A microbial community study using a culture-dependent method was conducted on dehydrated sludge collected from a steel factory’s wastewater treatment plant. One isolate, designated QWL-01^T, was a strictly anaerobic, Gram-stain-negative, non-motile, non-spore-forming bacterium with coccoid cells measuring 0.6–0.9 μm in diameter. The growth of strain QWL-01^T was observed at 4–40 °C (optimum at 28–35 °C), pH 5.5–8.0 (optimum at pH 7.1), and a range of 0–3% NaCl (optimum at 0.5%). An analysis of the Biolog AN plate revealed positive carbon source utilization only for palatinose, α-ketovaleric acid, and pyruvic acid. The predominant fatty acids were iso-C_13:0 (17.0%), C_16:0 dimethyl acetal (12.0%), and anteiso-C_13:0 (9.2%). A 16S rRNA gene sequence analysis through BLASTN demonstrated that the nearest phylogenetic neighbors of the novel strain were Youngiibacter multivorans DSM 6139^T (93.82%) and Proteiniclasticum ruminis JCM 14817^T (93.75%). The genome size of strain QWL-01^T was 3.69 Mbp, with a G+C content of 50.8 mol%. Comparing strain QWL-01^T with closely related species of genera Proteiniclasticum and Youngiibacter, the digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI), and average amino acid identity (AAI) values ranged from 26.60% to 36.80%, 65.89% to 68.30%, and 49.27% to 51.58%, respectively. Based on phenotypic, physiological, phylogenetic, and genomic relatedness evidence, strain QWL-01^T represents a novel genus in the family Clostridiaceae, for which the name Anaerococcoides asporogena gen. nov. sp. nov. is proposed. Strain QWL-01^T (=BCRC 81396^T = CICC 25258^T = NBRC 117088^T) is the type strain of the proposed novel species.

Here, we report the complete genome of Sedimentibacter strain MB31-C6 (=BCRC 81431), isolated from sewage sludge at the Sanming Steel wastewater treatment plant, China. The strain harbors a 3,009,901 bp circular chromosome (30.1% GC) and a 13,652 bp plasmid, which were used for further species delineation and comparative genomic analyses.

Here, we report the complete genome sequence of Oscillospiraceae bacterium MB24-C1 (= BCRC 81428), isolated from sewage sludge of the Sanming Steel wastewater treatment plant, China. The genome comprises 2,952,321 bp with a GC content of 48.1% and was used for further species delineation and comparative genomic analyses.

Here, we report the complete genome sequence of Bacteroidales bacterium strain MB20-C3-3 (=BCRC 81427) isolated from sewage sludge. The genome is 2,379,874 bp in length with a guanine and cytosine content of 40.0%. The genome of strain MB20-C3-3 was used for further species delineation and comparative genomic analyses.

Here, we report the complete genome sequence of Oscillospiraceae bacterium MB08-C2-2 (=BCRC 81394), isolated from feces of black soldier fly ( Hermetia illucens ) larvae, comprising 3,292,498 bp, a GC content of 49.67%, and identification of a 72,574 bp plasmid for further species delineation and comparative genomic analysis.

The gut microbiota of Tenebrio molitor has attracted attention for its potential in solving plastic waste problems. Here, we report the complete genome sequence of Propionimicrobium sp. PCR01-08-3, isolated from the feces of Tenebrio molitor larvae. The genome of strain PCR01-08-3 was selected for further species delineation and comparative genomic analysis.

A novel mesophilic, hydrogenotrophic methanogen, strain CWC-01^T, was isolated from the sediment sample of the piston core collected at station 3 at Good Weather Ridge area offshore southwestern Taiwan from ORI-902A cruise in 2009. Cells of strain CWC-01^T are rod-shaped, measuring 0.4–0.5 µm in width and 1.0–2.0 µm in length. Strain CWC-01^T utilizes formate and H₂/CO₂ to produce methane, but not secondary alcohol, methanol, ethanol, methylamines or acetate as catabolic substrates. The optimal growth conditions are 37 °C, 0.043 M NaCl and pH 6.3. Growth effectors tests indicate tryptone, but not acetate and yeast extract, could stimulate the growth of strain CWC-01. Phylogenetic analysis of 16S rRNA gene reveals that strain CWC-01^T is most closely related to Methanobacterium petrolearium Mic5c12^T, with 96.63% identity. Genome size of strain CWC-01^T is 1.98 Mb and it is the smallest genome size of genus. The genomic DNA G+C content obtained from the genome sequence of strain CWC-01^T is 44.15 mol%. Based on these phenotypic, phylogenetic and genomic analyses, we propose that strain CWC-01^T represents a novel species in genus Methanobacterium, for which the name Methanobacterium aridiramus sp. nov. is proposed. The type strain is CWC-01^T (=BCRC AR10053^T=NBRC 113991^T).