Hyun Jung Lee

Background: Previous research has demonstrated that the use of continuous glucose monitoring (CGM) can improve glycemic control in people with type 2 diabetes when used regularly alongside a digital diabetes self-management education and support (DSMES) program. However, to date, there is limited evidence showing the benefits of a digitally delivered DSMES program combined with real-time CGM for adults with type 2 diabetes. Objective: The objective of this study is to evaluate the impact of a DSMES program coupled with CGM on hemoglobin A _1c (HbA _1c ) and CGM-derived glycemic measures compared to usual care for adults with type 2 diabetes over 6 months. Methods: Participants with type 2 diabetes and HbA _1c of 8% or higher (64 mmol/mol) who were not using mealtime bolus insulin (aged 26‐83 y; mean HbA _1c 9.6%, SD 1.4% [mean 81.2 mmol/mol, SD 15.8 mmol/mol]) were randomly assigned to a digital DSMES+CGM integrated solution (n=51) or usual care (n=49) for 6 months. The primary outcome was HbA _1c . The secondary outcomes were CGM-derived glycemic measures, including glucose management indicator, percent time in range 70 to 180 mg/dL, percent time above range (>180 mg/dL), percent time below range (1c was lower among the intervention group versus the usual care group at 3 months (difference=−0.7%, 95% CI −1.4% to −0.1% or difference=−8.1 mmol/mol, 95% CI −15.5 to −0.7 mmol/mol; P =.03) and at 6 months (difference=−0.6%, 95% CI −1.4% to 0.2% or difference=−6.9 mmol/mol, 95% CI −15.7 to 1.9 mmol/mol; P =.12) but only reached statistical significance at 3 months. CGM-derived glycemic measures, including glucose management indicator (difference=−0.9%, 95% CI −1.7% to −0.1%; P =.03), time in range (difference=14.6%, 95% CI 1.0% to 28.2%; P =.04), time above range (difference=−14.9%, 95% CI −29.0% to −0.9%; P =.04), and mean glucose (difference=−36.4 mg/dL, 95% CI −70.0 to −2.9 mg/dL; P =.03), also significantly improved for the intervention group versus the usual care group at 6 months. Conclusions: The combination of digital DSMES+CGM is effective for supporting adults with type 2 diabetes in managing their condition and has the potential to reduce the risk of long-term health complications.

Obesity is well-known as a major risk factor for metabolic disorders, and natural compounds are being explored as alternatives to conventional therapies. While Centella asiatica is well known for its medicinal and dietary benefits, the biological activities of Giant Centella asiatica (GCA), especially when extracted with mineral-rich lava seawater, remain poorly characterized. This study aimed to evaluate the anti-adipogenic and lipid-metabolism-regulating effects of a novel GCA extract (GCA-LS-90) and its ability to stimulate GLP-1 secretion in vitro. GCA-LS-90 significantly inhibited lipid accumulation in 3T3-L1 adipocytes by up to 24.3% at 200 µg/mL (p < 0.001). It downregulated adipogenic transcription factors (C/EBPβ, C/EBPα, PPARγ) and lipogenic regulators (SREBP1c, FAS, G6PD, ME), while upregulating KLF2 (all p < 0.001). Western blotting confirmed reduced SREBP1c and SREBP2 protein expression, increased phosphorylation of AMPKα/ACC, and enhanced HSL activity (p < 0.05–0.001). In STC-1 cells, GCA-LS-90 increased GLP-1 secretion (53.5 pmol/L at 90 µg/mL vs. 41.3 pmol/L in control, p < 0.001). The major compounds, 3,5- and 4,5-di-O-caffeoylquinic acids, reproduced these effects. In conclusion, GCA-LS-90 modulated adipogenesis-, lipid-metabolism-, and GLP-1 secretion-related pathways in vitro, suggesting its potential as a functional ingredient for obesity management. Further in vivo studies are needed to confirm efficacy and translational relevance.

Ochratoxin A (OTA), a potent nephrotoxin, is one of the most deleterious mycotoxins, with its prevalence in agricultural crops and their processed foods around the world. OTA is a major concern to food safety, as OTA exposure through dietary intake may lead to a significant level of accumulation in the body as a result of its long half-life (about 35 days). Its potent renal toxicity and high risk of exposure as well as the difficulty in controlling environmental factors OTA production has prompted the need for timely information on practical strategies for the food industry to effectively manage OTA contamination during food processing. The effects of various food processes, including both nonthermal and thermal methods, on the reduction in OTA were summarized in this review, with emphasis on the toxicity of residual OTA as well as its known and unknown degradation products. Since complete removal of OTA from foodstuffs is not feasible, additional strategies that may facilitate the reduction in OTA in food, such as adding baking soda and sugars, was also discussed, so that the industry may understand and apply practical measures to ensure the safety of its products destined for human consumption.

Hydrogen peroxide (H₂O₂) is a food additive for bleaching and sterilization, owing to its strong oxidizing effect. The current study aimed to develop analytical methods to detect trace amounts of residual H₂O₂ in diverse foods using high-performance liquid chromatography (HPLC) equipped with diode array detector (DAD) or fluorescence detector (FLD). The vanadium(V)-peroxo complex, derived from the reaction of H₂O₂ with ammonium metavanadate, was used for the detection of H₂O₂ with DAD. H₂O₂ was indirectly analyzed using FLD via the detection of 7-hydroxycoumarin, derived by Fenton reaction, followed by verification using liquid chromatography with tandem mass spectrometry analysis. Both the detection methods showed good linearity with R² > 0.997. Limit of detection and limit of quantification were 0.30 and 0.91 mg/L (8.82 and 26.76 μM) with HPLC–DAD and 0.001 and 0.003 mg/L (0.03 and 0.09 μM) with HPLC–FLD, respectively. Applicability of both the methods was successively tested through sample analysis.

Ochratoxin A (OTA) is a possible human carcinogen commonly found in various agricultural commodities worldwide. While this potent nephrotoxin tends to survive common food processes and contaminate food products, certain process with higher temperature treatments may reduce OTA contents. Roasting has been suggested as a possible method to reduce OTA in coffee beans with up to 90% reduction, which may be applied to other food commodities. In this study, the possible influence of fibres on the reduction of OTA was investigated with brown and white rice with 2.2 and 6.7% of total dietary fibre content, respectively, roasting at two different temperatures (160 and 200 °C) for up to 30 min. The results showed that the rate and extent of OTA reduction were dependent on time, temperature, and rice type; greater than 60% of OTA reduction were achieved at 200 °C for 30 min for white rice and 37% for brown rice at the same conditions. No significant differences in reduction were observed between the samples roasted at 160 °C for 30 min and 200 °C for 15 min for both the white and brown rice, while white rice roasted at 160 °C for 15 min during roasting may be affected by the presence of fibre and/or fat in the cereal grains.

Ochratoxin A (OTA) is a mycotoxin that is potentially carcinogenic to humans. Although its mechanism remains unclear, oxidative stress has been recognized as a plausible cause for the potent renal carcinogenicity observed in experimental animals. The effect of OTA on oxidative stress parameters in two cell lines of LLC-PK1 and HK-2 derived from the kidneys of pig and human, respectively, were investigated and compared. We found that the cytotoxicity of OTA on LLC-PK1 and HK-2 cells was dose- and time-dependent in both cell lines. Furthermore, increased intracellular reactive oxygen species (ROS) induced by OTA in both cell lines were observed in a time-dependent manner. Glutathione (GSH) was depleted by OTA at >48 h in HK-2 but not in LLC-PK1 cells. While the mRNA levels of glucose-6-phosphate dehydrogenase (G6PD) and glutathione peroxidase 1 (GPX1) in LLC-PK1 were down-regulated by 0.67- and 0.66-fold, respectively, those of catalase (CAT), glutathione reductase (GSR), and superoxide dismutase 1 (SOD) in HK-2 were up-regulated by 2.20-, 2.24-, and 2.75-fold, respectively, after 72 h exposure to OTA. Based on these results, we conclude that HK-2 cells are more sensitive to OTA-mediated toxicity than LLC-PK1, and OTA can cause a significant oxidative stress in HK-2 as indicated by changes in the parameter evaluated.

Spent coffee grounds (SCG) are inexpensive materials that have been used as a source of antioxidants and polysaccharides with immunostimulatory activity. In this study, we performed a microbial fermentation of SCG using Cordyceps sinensis and investigated the radical scavenging and immunostimulatory activity of fermented SCG. SCG fermentation using C. sinensis was performed at 25 °C for 8 d. The polyphenol content of the fermented SCG increased from 1022.4 to 1562.0 μg/mL. The glucosamine content of the mycelia also continuously increased during fermentation. The main polyphenol compounds of fermented SCG were chlorogenic acid and p-coumaric acid, which were increased by fermentation. Fermented SCG also showed significantly higher content of chlorogenic acid isomers than unfermented SCG. The fermented SCG exhibited significantly higher 2,2-diphenyl-2-picrylhydrazyl hydrate (half maximal inhibitory concentration: IC₅₀, 0.37 mg/mL) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (IC₅₀, 0.93 mg/mL) radical scavenging activities than those of the control (0.54 mg/mL and 1.20 mg/mL, respectively; p < 0.05). The fermented SCG stimulated macrophages and promoted the production of various immunostimulatory cytokines (IL-12, IL-6, and TNF-α) compared to control; therefore, microbial fermentation of SCG using C. sinensis is an effective means of generating antioxidant and immunostimulatory materials.